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Histochemical Staining Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/H2.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for histochemical staining. This protocol has been optimized for b-galactosidase and human placental alkaline phosphatase staining in retinal tissue and cultured cells. - [Read Histochemical Staining Protocol]

Lambda DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D3.html

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Protocol describes a plate method that gives very good yield for cloning. Includes: T-TYN Media + Mg+2; T-TYN Plates; T-TYN Top Agarose. - [Read Lambda DNA Preparation Protocol]

Lambda DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D3.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for lambda DNA preparation. This is a plate method that gives very good yield for cloning. - [Read Lambda DNA Preparation Protocol]

Lambda gt22a Screening Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/L1.html

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Protocol for lambda gt22a screening. Includes: T-TYN Media + Mg+2; T-TYN Plates; T-TYN Top Agarose; Phage Dilution Buffer (PDB); 10X Kinase Buffer (-DTT); Calf Thymus DNA. - [Read Lambda gt22a Screening Protocol]

Maxam Gilbert Sequencing for DnaseI Footprinting Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/M2.html

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Quick protocol to give a representative G+A ladder for DnaseI footprint experiments. - [Read Maxam Gilbert Sequencing for DnaseI Footprinting Protocol]

Methylation Interference Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/M1.html

Category: DNA Protein Interactions Protocols

Protocol for methylation interference. - [Read Methylation Interference Protocol]

Nuclear Extract Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/N1.html

Category: DNA Protein Interactions Protocols

Protocol for nuclear extract preparation. - [Read Nuclear Extract Preparation Protocol]

Oligonucleotide Purification Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/O1.html

Category: Nucleic Acid Purification Protocols

Protocol for oligonucleotide purification. - [Read Oligonucleotide Purification Protocol]

PCR Genotyping from Tail DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/P4.html

Category: Genetics and Genomics: Genotyping Protocols: PCR Genotyping

Protocol for PCR genotyping from tail DNA. This protocol works well for a variety of genes and primer pairs including Tg and KO alleles. Oligonucleotide melting temperatures between 60° and 65° seem to work well. - [Read PCR Genotyping from Tail DNA Protocol]

PCR Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/P3.html

Category: PCR Protocols: Standard PCR Protocol

Protocol for PCR. - [Read PCR Protocol]

Plasmid Isolation and Analysis. Pictorial Protocol. - http://www.biotech.iastate.edu/publications/ppt_presentations/html/IsoPart1_files/v3_document.htm

Category: General Research Protocols and Methods

Plasmid Isolation and Analysis. Pictorial Protocol. Mike Zeller. - [Read Plasmid Isolation and Analysis. Pictorial Protocol.]

Plasmid Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S1.html

Category: DNA Protocols: DNA Sequencing Protocols

Protocol for plasmid sequencing. - [Read Plasmid Sequencing Protocol]

Protocol for Stable Lines - http://genetics.med.harvard.edu/~cepko/protocol/mike/S4.html

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This protocol is optimized for erythroid cells, other cell types may require modifications. - [Read Protocol for Stable Lines]

Quick DNA Plasmid Prep Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D4.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for quick DNA plasmid prep. Protocol gives very clean plasmid preps for restriction digests and cloning. However, due to the alkaline lysis step, the DNA is often nicked and may not give exceptional sequence data. - [Read Quick DNA Plasmid Prep Protocol]

Quick DNA Plasmid Prep. Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D2.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for quick DNA plasmid prep. This is a very fast mini-prep protocol which is suitable for sequence analysis and restriction digests. - [Read Quick DNA Plasmid Prep. Protocol]

Random Prime Labeling of DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/R1.html

Category: Nucleic Acid Modification Protocols: Random Primer Labeling Protocols

Protocol for random prime labeling of DNA. - [Read Random Prime Labeling of DNA Protocol]

RNA Preparation from Cultured Cells or Tissue Samples Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/R2.html

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols: RNA Isolation fromTissues or Cultured Cells Protocols

Protocol for RNA preparation from cultured cells or tissue samples. This protocol has been used to isolate RNA from relatively small tissue samples. The RNA is clean enough for Rnase protection, cDNA synthesis, and RT-PCR analysis. - [Read RNA Preparation from Cultured Cells or Tissue Samples Protocol]

Rnase Protection Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/R3.html

Category: Molecular Biology Protocols

Protocol for RNAse protection assay. - [Read Rnase Protection Assay Protocol]

RT-PCR Analysis Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/R4.html

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

Protocol for RT-PCR analysis. - [Read RT-PCR Analysis Protocol]

Silinization of Slides Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S6.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for silinization of slides. - [Read Silinization of Slides Protocol]

Single Strand DNA Prep for Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S2.html

Category: DNA Protocols: DNA Sequencing Protocols

Protocol for single strand DNA prep for sequencing. Protocol works well from a 5 ml starting culture or a 1 ml starting culture. - [Read Single Strand DNA Prep for Sequencing Protocol]

Single Strand DNA Prep For Sequencing Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S2.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for single strand DNA prep for sequencing. This protocol works well from a 5 ml starting culture or a 1 ml starting culture (adjust volume accordingly). - [Read Single Strand DNA Prep For Sequencing Protocol]

Southern Blotting Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S5.html

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southern blotting. - [Read Southern Blotting Protocol]

Southwestern Blot Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/S3.html

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for southwestern blot. - [Read Southwestern Blot Protocol]

Step by Step Lab Protocols using pictures - http://www.biotech.iastate.edu/publications/ppt_presentations/default.html

Category: General Research Protocols and Methods

Step by Step Lab Protocols using pictures. Mike Zeller. - [Read Step by Step Lab Protocols using pictures]

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