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Agarose Gel Preparation Pictures - http://www.biotech.iastate.edu/publications/ppt_presentations/html/Fingerprinting/AgaroseGelPrep/02.html

Category: General Research Protocols and Methods

Agarose Gel Preparation Pictures. Mike Zeller - [Read Agarose Gel Preparation Pictures]

Antibody Purification - http://axon.med.harvard.edu/~cepko/protocol/mike/A1.html

Category: Protein Protocols: Antibody Purification Protocols

This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Mike A. Dyer Lab Harvard. - [Read Antibody Purification]

Antigen Retrieval - Immunohistochemistry - http://axon.med.harvard.edu/~cepko/protocol/mike/A2.html

Category: Immunology: Antigen Retrieval

Denaturing Solution and Procedure for AR. Mike Dyer. - [Read Antigen Retrieval - Immunohistochemistry]

Antigen Retrieval Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/A2.html

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol for antigen retrieval. - [Read Antigen Retrieval Protocol]

b-galactosidase Solution Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/B2.html

Category: Immunohistochemistry Protocols

Protocol for b-galactosidase solution assay. - [Read b-galactosidase Solution Assay Protocol]

Baculovirus Production for Recombinant Proteins - http://axon.med.harvard.edu/~cepko/protocol/mike/B1.html

Category: Protein Protocols: Protein Expression Protocols

Baculovirus Production for Recombinant Proteins. Protocol is a modified version of the pharmingen Baculogold kit. Brian Dynlacht. Mike A. Dyer Lab. - [Read Baculovirus Production for Recombinant Proteins]

Chromosomal DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C3.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Protocol for chromosomal DNA preparation. Protocol was developed for cultured cells but should be appropriate for dissociated tissues as well. - [Read Chromosomal DNA Preparation Protocol]

Conditioned Medium for Retinal Explant Culture Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C6.html

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Neuronal Cell Culture Media Protocols

Protocol for conditioned medium for retinal explant culture. - [Read Conditioned Medium for Retinal Explant Culture Protocol]

COS Cell Transfection Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C5.html

Category: Transfection Protocols

COS cell transfection protocol. Includes solutions: DEAE Dextran and 1000X Chloroquine - [Read COS Cell Transfection Protocol]

Cryosectioning Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C7.html

Category: Immunohistochemistry Protocols: Cryosectioning Protocols

Protocol for cryosectioning. While the timing of the various steps in this protocol are probably not critical, process the tissue all at once to ensure that RNA and/or proteins do not get degraded. Includes: 20% Paraformaldehyde/4% Paraformaldehyde-PBS; Sucrose/PBS. - [Read Cryosectioning Protocol]

CsCl Prep of Plasmid DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for CsCl prep of plasmid DNA. This is a standard large scale prep. For plasmid DNA which gives a yield of 0.5 1.0 mg. - [Read CsCl Prep of Plasmid DNA Protocol]

Dissociated Retina Immunohistochemistry Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D6.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retina immunohistochemistry. - [Read Dissociated Retina Immunohistochemistry Protocol]

Dissociated Retinal Cell Scoring Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/score.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retinal cell scoring. Includes: Rods; Cones; Bipolars; Müller Glial Cells; Pan-Amacrine/Horizontal. - [Read Dissociated Retinal Cell Scoring Protocol]

DNA Fragment Purification from Agarose or Acrylamide - http://axon.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: DNA Protocols: DNA Extraction Protocols: DNA Extraction Polyacrylamide Gels

DNA Fragment Purification from Agarose or Acrylamide. The protocol for fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose or Acrylamide]

DNA Fragment Purification from Agarose or Acrylamide Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for DNA fragment purification from agarose or acrylamide. For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose or Acrylamide Protocol]

DNA Fragment Purification from Agarose Protocol - http://axon.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

DNA Fragment Purification from Agarose Protocol. This protocol is best for fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose Protocol]

DNA Plasmid Prep. Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for DNA plasmid prep. - [Read DNA Plasmid Prep. Protocol]

DnaseI Footprint Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F2.html

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol for DnaseI footprint. - [Read DnaseI Footprint Protocol]

EMSA using ds Oligonucleotides - http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

EMSA probe creation using ds Oligonucleotides by annealing two complementary oligos, and Klenow for probe creation. - [Read EMSA using ds Oligonucleotides]

EMSA using ds Oligonucleotides Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/E1.html

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol for EMSA using ds oligonucleotides. - [Read EMSA using ds Oligonucleotides Protocol]

Expression and Purification of His Tagged Proteins in E. coli Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/H1.html

Category: E Coli Protocols: E coli Protein Protocols

Protocol for the expression and purification of His tagged proteins in E. coli. - [Read Expression and Purification of His Tagged Proteins in E. coli Protocol]

Fill-in Labeling of DNA Fragments Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F1.html

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for fill-in labeling of DNA fragments. - [Read Fill-in Labeling of DNA Fragments Protocol]

Frozen Competent E. coli Preparation and Transformation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F3.html

Category: E Coli Protocols: E coli Transformation Protocols

Protocol for frozen competent E. coli preparation and transformation. - [Read Frozen Competent E. coli Preparation and Transformation Protocol]

Frozen Competent E. coli Preparation and Transformation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F3.html

Category: Competent Cells Protocols

This is a very easy protocol which yields transformation efficiencies appropriate for routine cloning and transformation. - [Read Frozen Competent E. coli Preparation and Transformation Protocol]

High Efficiency FCC Preparation and Tx Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F4.html

Category: Competent Cells Protocols

This is an easy and straightforward protocol that gives efficiencies of 106 to 107 cfu/mg of plasmid DNA. A growth curve is required for each strain that is prepared.
- [Read High Efficiency FCC Preparation and Tx Protocol]

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