microtubule Protocols

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Making Microtubule Seeds Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mtseeds.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for making microtubule seeds. - [Read Making Microtubule Seeds Protocol]

Microtubule Assembly Protocol - http://www.proweb.org/kinesin/Methods/MT_assembly.html

Category: Cell Biology and Cell Culture

Protocol on microtubule assembly using purified tubulin. - [Read Microtubule Assembly Protocol]

Microtubule Staining Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/microtubstain.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for microtubule staining. - [Read Microtubule Staining Protocol]

Microtubule/ organelle Motility Assays - http://www.bio.unc.edu/faculty/salmon/lab/protocolsmtorganellemotilityassays.html

Category: Cell Biology and Cell Culture

Microtuble/ organelled motility assay using Golgi or ER membranes, 45 uM tubulin, rat liver cytosol, and 20x energy regeneration system. - [Read Microtubule/ organelle Motility Assays]

Minichromosome Microtubule BInding Assay Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mmb.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for minichromosome microtubule binding assay. - [Read Minichromosome Microtubule BInding Assay Protocol]

Negative Stain Electron Microscopy of Microtubules Protocol - http://mitchison.med.harvard.edu/protocols/EM.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains, structural artifacts such as flattening of the cylindrical microtubule and opening up of microtubules into flat sheets are common. Negative staining is very useful because of its ease, rapidity and lack of requirement for specialized equipment other than that found in a regular EM facility. - [Read Negative Stain Electron Microscopy of Microtubules Protocol]

Protocol for Microtubule Binding Assays - http://www.proweb.org/kinesin/Methods/MT-binding_assay.html

Category: Cell Biology and Cell Culture

Microtubule binding assay protocol using the materials: Siliconized ultracentrifuge microfuge tubes, GTP-depleted microtubules, 6X SDS loading dye, 1X SDS loading dye, Coomassie Brilliant Blue R 250 (0.8% in 50% methanol + 10% acetic acid)and Destaining solution (13% methanol + 3% acetic acid). - [Read Protocol for Microtubule Binding Assays]

Protocol for Cycling Tubulin - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/cyclingmicrotub.html

Category: Cell Biology and Cell Culture

Protocol for cycling tubulin using the buffers Glycerol PB and BRB80. - [Read Protocol for Cycling Tubulin]

Protocol for Microtubule Assembly - http://www.bio.com/protocolstools/protocol.jhtml?id=p1600

Category: Cell Biology and Cell Culture

Purified Tubulin is polymerized and then stabilized with Taxol. The resulting Microtubules are then ready for use in motility assays. Protocol use solutions such as 50 mM Mg-GTP, 50 Î¼l 100 mM GTP, 40 Î¼M Taxol, Taxol Stock,and PM Buffer. - [Read Protocol for Microtubule Assembly]

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Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.