microsomal Protocols

Search results for: microsomal

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

In vitro Translation Assays for CFTR - http://pen2.igc.gulbenkian.pt/cftr/vr/c/ostedgaard_in_vitro_translation_assays_for_cftr.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol outlines the general procedure and requirements for in vitro translation of CFTR and outlines some assays using in vitro translated product. Core glycosylation of CFTR occurs in the ER. An assay for this processing step requires the addition of microsomal membranes to the basic in vitro translation mixture. This protocol takes this into account. - [Read In vitro Translation Assays for CFTR]

Microsome Preparation from Yeast Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1676

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Microsome Isolation Protocols

This protocol describes the isolation of microsomal Endoplasmic Reticulum (ER) membranes from yeast cells. Includes: Spheroplast Formation and Microsome Purification. - [Read Microsome Preparation from Yeast Cells Protocol]

Single-tube Coupled Transcription/Translation Reactions for Eukaryotic In Vitro - http://www.promega.com/tbs/tm045/tm045.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Information for protocol using single-tube, coupled transcription/translation reactions for eukaryotic in vitro translation. Includes information on: Translation Procedure; Positive Control Translation Reactions Using Luciferase; Cotranslational Processing Using Canine Pancreatic Microsomal Membranes; Post-Translational Analysis; Positive Control Luciferase Assays; Composition of Buffers and Solutions; Luciferase SP6/T7 Control DNAs - [Read Single-tube Coupled Transcription/Translation Reactions for Eukaryotic In Vitro]