microscopic Protocols

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Electron Microscopy of the Cytoskeleton of Cultured Cells - http://www.borisylab.northwestern.edu/pages/protocols/electmicrosc.html

Category: Microscopy Protocols: Electron Microscopy Protocols

Article describe the preparation of cells for correlative electron microscopy after live light microscopic observation of fluorescently labeled cytoskeletal proteins microinjected into the same cells. Since identification of cytoskeletal elements in electron microscopic preparations is an essential part of any correlative study, procedures for immunogold labeling of cytoskeletal components and for myosin S1 decoration of actin filaments are also described. - [Read Electron Microscopy of the Cytoskeleton of Cultured Cells]

Gold labeling of proteins for electron microscopy - http://www.biochem.wisc.edu/inman/empics/gold.htm

Category: Molecular Imaging: Electron Microscopy Protocols

In brief, the reaction mixture, is prepared in exactly the same way as if the sample is to be stained or shadowed for electron microscopy, but once the... Gold labeling technique for electron microscopic identification and location of proteins. - [Read Gold labeling of proteins for electron microscopy]

How to Do Film and Digital Imaging of Microscopic Preparations - http://www.fgsc.net/neurosporaprotocols/How%20to%20do%20film%20and%20digital%20imaging%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to do film and digital imaging of microscopic preparations. - [Read How to Do Film and Digital Imaging of Microscopic Preparations]

How to Prepare Ascus Rosettes for Microscopic Examination - http://www.fgsc.net/neurosporaprotocols/How_to_obtain_rosettes%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to prepare ascus rosettes for microscopic examination. - [Read How to Prepare Ascus Rosettes for Microscopic Examination]

Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip18

Category: Microscopy Protocols: In Vivo Imaging Protocols

Live nematodes can be studied at all levels of microscopic resolution. Data collection includes information on: Photographic Records, Video Microscopy,Multiple-Focal-Plane Time-Lapse Recording Systems and Digital Imaging. - [Read Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection]

Live Imaging of Caenorhabditis elegans: Preparation of Samples - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4601

Category: Microscopy Protocols: In Vivo Imaging Protocols

Caenorhabditis elegans, a small (adults are ~1 mm long), free-living soil nematode that feeds on bacteria, is an ideal organism for applying various live microscopy techniques. This protocol describes useful techniques for preparing C. elegans for live microscopic analysis. Details of sample preparation depend on the developmental stage of the worm to be studied. - [Read Live Imaging of Caenorhabditis elegans: Preparation of Samples]

Live Imaging of Caenorhabditis elegans: Preparation of Samples - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4601

Category: C. elegans Protocols

Protocol describes useful techniques for preparing C. elegans for live microscopic analysis. Details of sample preparation depend on the developmental stage of the worm to be studied. - [Read Live Imaging of Caenorhabditis elegans: Preparation of Samples]

Microscopic Analysis of Mitochondrial Transmembrane Potential Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4462

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes a method for the evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry. - [Read Microscopic Analysis of Mitochondrial Transmembrane Potential Protocol]

Neuroscience Histology Methods - http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Materials for cresyl violet staining protocol (see methods section) ... Histology also refers to the techniques used to prepare tissue for microscopic study ... Neuroscience Histology Methods - [Read Neuroscience Histology Methods]

Sorting Aberrant Cells Using Anti-Cytokeratin or DNA Content for Study of Cell Morphology - http://www.bdbiosciences.com/pdfs/whitePapers/23-2786-02.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Demonstrates the use of the FACSort for sorting based on DNA ploidy or the presence of cytokeratin, and the suitability of the sorted cells for morphologic analysis by microscopic examination. - [Read Sorting Aberrant Cells Using Anti-Cytokeratin or DNA Content for Study of Cell Morphology]

Total Internal Reflection Microscopy - http://www.andrew.cmu.edu/user/dcprieve/TIRM.htm

Category: Microscopy Protocols: Optical Microscopy Protocols

TIRM is a optical technique for monitoring the instantaneous separation distance between a microscopic sphere & a flat plate. Changes in distance as small as 1 nm can be detected. Includes information on: Scattering Intensity I is Related to Elevation h ; apparatus. - [Read Total Internal Reflection Microscopy]