microfuge Protocols

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Large-Scale Immunocytology Protocol - http://ygac.med.yale.edu/mtn/Immu_protocols.stm

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes. The protocol can also easily be adapted for preparing cells for immunofluorescence in microfuge tubes. - [Read Large-Scale Immunocytology Protocol]

Nematode Dye Filling Protocol - http://www.k-state.edu/hermanlab/protocols/nematode_dye_filling.htm

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for nematode dye filling. Includes: Thumb plate; Microfuge tube; 15 ml tube; Viewing: Big Scope and Dissecting Scope. - [Read Nematode Dye Filling Protocol]

Protocol for Microtubule Binding Assays - http://www.proweb.org/kinesin/Methods/MT-binding_assay.html

Category: Cell Biology and Cell Culture

Microtubule binding assay protocol using the materials: Siliconized ultracentrifuge microfuge tubes, GTP-depleted microtubules, 6X SDS loading dye, 1X SDS loading dye, Coomassie Brilliant Blue R 250 (0.8% in 50% methanol + 10% acetic acid)and Destaining solution (13% methanol + 3% acetic acid). - [Read Protocol for Microtubule Binding Assays]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.