microarray Protocols

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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

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Spotted Microarray Hybridization and Scan Protocol - http://www.genome.wisc.edu/resources/protocols/spottedarrayhybridization.htm

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol for spotted microarray hybridization and scan. Includes: Setup Hybridization; Wash Slides; Scan Slides. - [Read Spotted Microarray Hybridization and Scan Protocol]

Stripping DNA Microarray array for re-use reusing - http://www.oup.co.uk/pdf/pas/6-5-10.pdf

Category: DNA Microarray Protocols

Reusing DNA Microarrays using Stripping. Elder et al Oxford Practical Series. - [Read Stripping DNA Microarray array for re-use reusing]

Transcript Profiling by Microarray Analysis Protocol. - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray Hybridization Protocols

Protocol details the preparation of fluorescently labeled target samples and hybridization of these samples to a microarray of Agilent inkjet-deposited cDNAs. The procedure requires a minimum of 5 mg of purified total RNA as starting material. Includes: First Strand cDNA Synthesis; Second Strand cDNA Synthesis; cDNA Cleanup and Precipitation; In vitro Transcription; Cleanup and Quantification of in vitro Transcribed RNA; Fluorescent Labeling of the Target Samples. - [Read Transcript Profiling by Microarray Analysis Protocol.]

Transcript Profiling by Microarray Analysis—Agilent Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000019.pdf?pid=PP00000019

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Troubleshooting Arabidopsis Oligonucleotide Microarrays - http://ag.arizona.edu/microarray/Troubleshooting1.html

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Arabidopsis Oligonucleotide Microarrays, David Galbraith. - [Read Troubleshooting Arabidopsis Oligonucleotide Microarrays]

Troubleshooting Guide to Microarray Printing - http://arrayit.com/Products/Printing/Troubleshooting_Guide/troubleshooting_guide.html

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Troubleshooting Guide to Microarray Printing. Arrayit. - [Read Troubleshooting Guide to Microarray Printing]

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT - http://omrf.ouhsc.edu/~frank/M_QPCR-OMNISCRIPT-RT.html

Category: Real Time PCR

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT. Dr. Craig Cadwell - [Read Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.