michigan Protocols

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Colony PCR - http://www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/colony_pcr.html

Identification of Bacterial Colonies using PCR. Colony PCR Method and Technique. Maddock Lab, The University of Michigan. - [Read Colony PCR]

Genotyping Transgenic Rodents by PCR - http://www.med.umich.edu/tamc/pcr.html

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

Test mice and rats for the presence of the transgene by PCR. University of Michigan, Transgenic Animal Model Core - [Read Genotyping Transgenic Rodents by PCR]

Isolation of DNA from Mouse Tail Biopsies - http://www.med.umich.edu/tamc/tDNA.html

Category: Transgenic Mouse Protocols: Mouse Tail DNA Extraction Genotyping

Isolation of DNA from Mouse Tail Biopsies. University of Michigan Transgenic Animal Model Core, Transgenic Animal Web. - [Read Isolation of DNA from Mouse Tail Biopsies]

reparation of Copy Standards for Southern Blot Copy Number Determination - http://www.med.umich.edu/tamc/spike.html

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

PCR screens must be designed to detect transgene DNA at the single copy level.Copy standards are prepared by mixing non-transgenic tail DNA with a known amount of transgene DNA to produce transgene copy standards. University of Michigan Transgenic Animal - [Read reparation of Copy Standards for Southern Blot Copy Number Determination]

Timeline for Transgenic Mouse Analysis - http://www.med.umich.edu/tamc/tgtime.html

Category: Transgenic Mouse Protocols

Timeline for Transgenic Mouse Analysis and production. University of Michigan Transgenic Animal Model Core. Transgenic Animal Web. - [Read Timeline for Transgenic Mouse Analysis]

Transgenic Mouse Breeding - http://www.med.umich.edu/tamc/breed.html

Category: Transgenic Mouse Protocols

Mouse Breeding Suggestions, ES Cell-Mouse Chimera Breeding, Record Keeping, Transmission Patterns. University of Michigan Transgenic Animal (Web) Model Core - [Read Transgenic Mouse Breeding]