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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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mRNA Purification - http://www.fhcrc.org/science/labs/breeden/Methods/mRNA_purification.html

Category: RNA Protocols: mRNA Extraction Protocols

mRNA Purification using oligo dT cellulose. Breeden Lab. - [Read mRNA Purification]

Near-Field Scanning Optical Microscopy - http://microscopy.fsu.edu/primer/techniques/nearfield/nearfieldintro.html

Category: Microscopy Protocols: Optical Microscopy Protocols

Diffraction-limited optical microscopy requires that the spatial resolution of an image is limited by the wavelength of the incident light & by the numerical apertures of the condenser & objective lens systems.The development of near-field scanning optical microscopy (scanning near-field optical microscopy) has allowed for a imaging technique that retains the various contrast mechanisms afforded by optical microscopy methods while attaining spatial resolution beyond the optical diffraction limit - [Read Near-Field Scanning Optical Microscopy]

Neuroscience Histology Methods - http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Materials for cresyl violet staining protocol (see methods section) ... Histology also refers to the techniques used to prepare tissue for microscopic study ... Neuroscience Histology Methods - [Read Neuroscience Histology Methods]

Neurospora Methods Protocols - http://www.fgsc.net/methods/stanford.html

Category: Fungi Protocols: Neurospora Protocols

Protocols for Neurospora methods. Includes: Standard strains; Crosses; Minimal medium; Color coding of media; Agar substrate for manipulation and isolation; Stock solutions of supplements; Mating-type tests; Preservation of stocks by silica gel; Cleaning of glassware; Control of mites. - [Read Neurospora Methods Protocols]

Nick translation for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/nick_translation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Nick translation for FISH. Sanger Institute. - [Read Nick translation for FISH]

Nuclei Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplnuc.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol for nuclei isolation. - [Read Nuclei Isolation Protocol]

NZ (200 Ml/500 Ml) Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/stocksgl.html

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for NZ (200Ml/500Ml). - [Read NZ (200 Ml/500 Ml) Protocol]

Optimization of Elution Conditions for Dye-Ligand Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4211

Category: Chromatography Protocols: Dye Ligand Affinity Chromatography Protocols

Protocol for the optimization of elution condition for dye-ligand affinity chromotography. Elution methods used in dye-ligand affinity chromatography may be either selective or nonselective in nature. Usually, selective elution methods are applied in combination with group-specific adsorbents, such as dye-ligand adsorbents, and nonselective elution methods are used in combination with highly specific adsorbents. - [Read Optimization of Elution Conditions for Dye-Ligand Affinity Chromatography Protocol]

Optimized Welsh DDRT-PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpcr2.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols

Optimized Welsh DDRT-PCR Protocol. Provided by Mirta Grifman and found in Neuroscience Protocols (Nov 1995). - [Read Optimized Welsh DDRT-PCR Protocol]

Optimized Welsh DDRT-PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpcr2.html

Category: PCR Protocols

Optimized Welsh DDRT-PCR Protocol. Provided by Mirta Grifman and found in Neuroscience Protocols (Nov 1995). - [Read Optimized Welsh DDRT-PCR Protocol]

Optimized Welsh DDRT-PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpcr2.html

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

Protocol for optimized welsh DDRT-PCR. - [Read Optimized Welsh DDRT-PCR Protocol]

PC12 Cell Culture and Fusion - http://ylwang.umassmed.edu/protocol/cc/pc12.htm

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

PC12 Cell Culture and Fusion Methods. Yu-li Wang - [Read PC12 Cell Culture and Fusion]

PCR based Lambda Î»DNA library screening method - http://www.methods.info/Methods/Library_screening/library_pcr.pdf

Category: RNA Protocols: cDNA Libraries Library

"useful screen phage library non-radioactive, and not need too many clones. Up to 5 clones can be obtained usually within one week." Methods.info - [Read PCR based Lambda Î»DNA library screening method]

PCR from Tissue - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PCR/directTissuePCR.html

Category: Plant Biology Protocols: Plant PCR

PCR from Tissue - [Read PCR from Tissue]

PCR From TIssue Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PCR/directTissuePCR.html/

Category: Plant Biology Protocols: Plant PCR

Protocol on how to perform a PCR from plant tissue (leaf or flower). - [Read PCR From TIssue Protocol]

PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION - http://wheat.pw.usda.gov/~lazo/methods/minn/chap-shoot2.fm.html

Category: PCR Protocols: In-situ PCR Protocols

PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION FOR THE DETECTION AND INVESTIGATION OF VIRAL LATENCY AND PERSISTENCE. Staskus 1998 U.Minn. - [Read PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION]

PEI Nanoparticles for Targeted Gene Delivery Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4451

Category: Gene Delivery Protocols: DNA Nanoparticles Protocols

This protocol describes the preparation of polyethylenimine (PEI)/DNA nanoparticles for targeted gene delivery. This delivery strategy improves the efficiency of gene transfer by enhancing the entry of gene vectors into the desired cells and reducing uptake by nontarget cells. We describe here methods for the conjugation of targeting peptides to PEIs, formation of DNA complexes using the conjugated PEIs or nonconjugated PEIs together with targeting peptides, and cell transfection. - [Read PEI Nanoparticles for Targeted Gene Delivery Protocol]

Phenol-based Protocol Isolation of DNA Fragments from Low-Melting Temperature Agarose - http://wheat.pw.usda.gov/~lazo/methods/101/101lmtagar.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

Phenol-based Protocol Isolation of DNA Fragments from Low-Melting Temperature Agarose. Reference: Favre, D. 1992. Biotechniques vol. 13. - [Read Phenol-based Protocol Isolation of DNA Fragments from Low-Melting Temperature Agarose]

Phosphate (Sodium) buffer Chart - http://wheat.pw.usda.gov/~lazo/methods/lazo/phosphat.html

Category: Buffers Media, and Solutions

Phosphate (Sodium) buffer Chart. Easy to setup buffers using this chart. Gerard R. Lazo - [Read Phosphate (Sodium) buffer Chart]

Phosphoamino acid analysis of non-TCA precipitable proteins - http://kinase.uhnres.utoronto.ca/methods/PAA.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Phosphoamino acid analysis of non-TCA precipitable proteins and Alkali treatment of gels protocols. Woodgett Lab. - [Read Phosphoamino acid analysis of non-TCA precipitable proteins]

Plant Crosses Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/PlantWork/crosses.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol describing on how to perform crosses in plants. - [Read Plant Crosses Protocol]

Plant DNA Extraction Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplmy.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysaccharide which inhibit restriction enzymes as well as other DNA modifying enzymes. This problem is most easily solved by using young plants which have not accumulated as much polysaccharide as older plants. The best results are obtained with plants that are two to three weeks post germinated. - [Read Plant DNA Extraction Protocol]

Plant tissue culture - http://www.oup.com/uk/orc/bin/9780199254682/ch02.pdf

Category: Plant Biology Protocols: Plant Tissue Culture

Integration of plant tissue culture into plant. transformation protocols. Various methods of plant regeneration are available to the plant biotechnol- ... - [Read Plant tissue culture]

POLY A+ RNA PREPARATION using proteinase K and SDS - http://wheat.pw.usda.gov/~lazo/methods/au/sect9.html

Category: RNA Protocols: mRNA Extraction Protocols

POLY A+ RNA PREPARATION using proteinase K and SDS. Bowtell Lab. - [Read POLY A+ RNA PREPARATION using proteinase K and SDS]

Polycrystalline thin films Sample Preparation Protocol MALDI - http://prowl.rockefeller.edu/methods/thinfilm.htm

Category: Mass Spectrometry Protocols

This method of sample preparation produces a uniform layer of very small crystals on the mass spectrometer's sample stage that are mechanically well adhered to the substrate. It is a variant of the dried droplet method. PROWL - [Read Polycrystalline thin films Sample Preparation Protocol MALDI]