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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Detection of Mutations by Single-strand Conformational Polymorphism Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3815

Category: Genetics and Genomics: Cancer Genetics Protocols: Single Strand Conformational Polymorphism(SSCP) Protocols

Single-strand conformational polymorphism (SSCP), one of several methods used to scan segments of DNA for mutations, exploits the electrophoretic differences in mobilities between single-stranded mutant and wild-type DNAs. - [Read Detection of Mutations by Single-strand Conformational Polymorphism Protocol]

Determination of the Extinction Coefficient for a Protein of Unknown Concentration - http://www.ruf.rice.edu/~bioslabs/methods/protein/absext.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Determination of the Extinction Coefficient for a Protein of Unknown Concentration. The concentration can be determined for a solution of a pure protein with unknown extinction coefficient. - [Read Determination of the Extinction Coefficient for a Protein of Unknown Concentration]

Differential cDNA Screening Procedures - http://wheat.pw.usda.gov/~lazo/methods/misc/Talbot.html

Category: RNA Protocols: cDNA Libraries Library

Differential cDNA Screening Procedures. The protocols listed refer to cDNA library construction and preliminary differential screening procedures. Nick Talbot lab - [Read Differential cDNA Screening Procedures]

DNA Extraction from Agarose Gels Protocol - http://www.methodbook.net/dna/gelextrc.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

DNA Extraction from Agarose Gels Protocol. The page includes cutting out the DNA band from the gel, and describes three methods including 1) Spin-columns (Nucleic acid purification columns), 2) using Dialysis tubing (semi-permeable membrane, Visking tubing), and the 3) Paper strip method.Matt Lewis, Department of Pathology, University of Liverpool. - [Read DNA Extraction from Agarose Gels Protocol]

DNA Extraction Procedure (CsCl) - Rhizobium - http://wheat.pw.usda.gov/~lazo/methods/lazo/rhizodna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol describing extraction of DNA from Rhizobium. - [Read DNA Extraction Procedure (CsCl) - Rhizobium]

DNA FIBERS FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/fiber_fish.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

DNA FIBERS FISH Protocol and Methods. Sanger Institute. - [Read DNA FIBERS FISH]

DNA Isolation from Onion Cells - http://www.accessexcellence.org/AE/AEC/AEF/1994/dollard_onionDNA.html

Category: DNA Protocols: DNA Extraction Protocols

Allows students to easily isolate chromosomal DNA from onion cells using the same basic tools and methods that scientists use in the lab. A good introduction to using pipettes. Kate Dollard - [Read DNA Isolation from Onion Cells]

DNA Sequencing Methods - http://www.genome.ou.edu/protocol_book/protocol_partIV.html#IV.C

Category: DNA Protocols: DNA Sequencing Protocols

Methods for DNA sequencing. Includes: Bst-catalyzed radiolabeled DNA sequencing; Radiolabeled sequencing gel preparation, loading, and electrophoresis; Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers; Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions; Sequenase[TM] catalyzed sequencing with dye-labeled terminators; etc. - [Read DNA Sequencing Methods]

DNA Transfection by Electroporation - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3751

Category: Transfection Protocols: Stable Transfection Protocols

Pulsed electrical fields can be used to introduce DNA into a wide variety of animal cells. Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate-DNA coprecipitation. But, as with other transfection methods, the optimal conditions for electroporating DNA into untested cell lines must be determined experimentally. - [Read DNA Transfection by Electroporation]

DNA Transfection Using Polybrene Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3875

Category: Transfection Protocols

Polybrene and DMSO can be used to achieve stable transformation of several types of cells by plasmid DNA. The yield of transformants is up to 15-fold greater with Polybrene than with calcium phosphate-DNA coprecipitation. However, there is no difference between the two methods in the efficiency of transformation of cells by high-molecular-weight DNA. - [Read DNA Transfection Using Polybrene Protocol]

DNaseI Footprinting Assay - http://www.fhcrc.org/science/labs/breeden/Methods/DNaseI-footprint.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

DNaseI Footprinting Assay. Works well high affinity dna binding proteins. Breeden Lab. - [Read DNaseI Footprinting Assay]

DOP PCR For CGH Array - http://www.sanger.ac.uk/HGP/methods/cytogenetics/DOPPCR.shtml

Category: Cytogenetics Protocols: Array CGH

DOP PCR Protocol For CGH Array. - [Read DOP PCR For CGH Array]

Dried droplet Sample Preparation for MALDI - http://prowl.rockefeller.edu/methods/dried.htm

Category: Mass Spectrometry Protocols

Dried droplet Sample Preparation for MALDI.The discovery of this method allowed the application of laser desorption to proteins [2]. Drying a droplet of a protein/matrix solution remains the favorite method of most MALDI practitioners. Protocol for dried - [Read Dried droplet Sample Preparation for MALDI]

Drosophila Single-Fly DNA Preps For PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafly.html

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila single-fly DNA preps for PCR. Includes: DNA Preparation Protocol; DNA Preps from many individual flies; Inverse PCR. - [Read Drosophila Single-Fly DNA Preps For PCR Protocol]

Early Events in B Lymphocyte Activation Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6634190D367003ECF94AE65295FA77&objectid=6674AB8295693316922D8DFC029193A6

Category: Immunology: B Cell Protocols

B cell activation can be quantitated indirectly by assaying antibody production or directly by measuring cellular changes that occur immediately after exposure to an activation signal. Provides methods for the latter (direct) approach--namely, methods for quantifying early parameters of B cell activation such as increases in intracellular ionized calcium concentration [Ca2+]I, cell size, and MHC class II-antigen expression. - [Read Early Events in B Lymphocyte Activation Protocol]

ELECTRON MICROSCOPY (EM) protocols and methods - http://www.bristol.ac.uk/vetpath/cpl/emtechs.htm

Category: Molecular Imaging: Electron Microscopy Protocols

E.M. processing schedule - epoxy resin E.M. processing schedule - acrylic resin Primary fixative and buffers Veterinary Pathology. ELECTRON MICROSCOPY (EM) protocols and methods - [Read ELECTRON MICROSCOPY (EM) protocols and methods]

Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4409

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol describes a method for electroporating DNA into ES cells, as well as selection methods. Pilot studies should be performed to optimize the conditions for each DNA construct. The selection method described here is one of the most complex. It involves targeting constructs in which the bacterial neomycin-resistance gene disrupts the coding sequence of the mouse gene. - [Read Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol]

Electroporation of Cell Lines With DNA - http://users.path.ox.ac.uk/~ciu/Methods/DNA/electroporation.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

Electroporation of Cell Lines With DNA. Electroporation for the efficient transfection of mammalian cells with DNA. Chu et al. Cellular Immunology Oxford - [Read Electroporation of Cell Lines With DNA]

ELISA (Enzyme-Linked ImmunoadSorbent Assay) - http://www.nottingham.ac.uk/~mbzspd/methods/ELISA.html

Category: ELISA Protocols

ELISA (Enzyme-Linked ImmunoadSorbent Assay) Method and technique. Jill Fergusson, Department of Biochemistry, University of Nottingham, - [Read ELISA (Enzyme-Linked ImmunoadSorbent Assay)]

ELISA Inhibition Assay - http://wheat.pw.usda.gov/~lazo/methods/goldberg/elisa.html

Category: ELISA Protocols

Protocol and method for ELISA Inhibition Assay. Michael Coyne. Goldberg Lab - [Read ELISA Inhibition Assay]

ELISA Method - http://mycoplasmas.vm.iastate.edu/lab_site/methods/immunol/ELISA.html

Category: ELISA Protocols

Reagents, protocol and Conjugate Preparation. Minion Lab, College of Veterinary Medicine, Iowa State. - [Read ELISA Method]

Embryo Lysates & Immunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/Precipitation.htm

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for embryo lysates and immunoprecipitation: protein A agarose & protein A magnetic beads. Includes: Embryo lysates; Immunoprecipitation with protein A agarose; Immunoprecipitation with protein A magnetic beads for silver stain/sequencing analysis. - [Read Embryo Lysates & Immunoprecipitation Protocol]

EMS Mutagenesis of Yeast Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/ems_mutagenesis.html

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for EMS mutagenesis of yeast. - [Read EMS Mutagenesis of Yeast Protocol]

Enrichment for Microsatellite Sequences in Onion (Allium cepa L.) Protocol - http://barley.ipk-gatersleben.de/~dirkf/netscape.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Microsatellite markers, also referred to as STMS (SequenceTagged Microsatellite Sites) or STR (Short Tandem Repeats) are widely used as molecular markers for intraspecific genotyping, molecular mapping and breeding purposes. The method described is an efficient,fast and relatively inexpensive way to obtain microsatellite markers without post-cloning selection methods. So far, the method has been successful in onion (Allium cepa L.), a plant with a large genome and for pathogenic fungi. - [Read Enrichment for Microsatellite Sequences in Onion (Allium cepa L.) Protocol]

Enrichment of PBMCs with Monocytes Protocol - http://www.methods.info/Methods/Cell_biology/monocyte_enrichment.pdf

Category: Immunology: Mononuclear Cell Isolation Protocols

Protocol for enrichment of PBMCs with monocytes. The cell suspension obtained after this protocol contains 40-70% monocytes. This cell suspension is than used for positive or negative MACS separation. - [Read Enrichment of PBMCs with Monocytes Protocol]