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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Antigen Retrieval PDF - http://www.dakousa.com/ishbantigenretr.pdf

Category: Immunology: Antigen Retrieval

Antigen Retrieval. Principles and Technique. A handbook of Immunochemical Staining Methods and techniques. Marc Key - [Read Antigen Retrieval PDF]

Apoptosis Methods Movies and Information - http://www.uni-greifswald.de/~immuteach/methods/tunel/tunel.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis Methods Movies and Information. Immuteach. - [Read Apoptosis Methods Movies and Information]

Apoptosis, Cell Death, And Proliferation. Roche Manual. - http://www.roche-applied-science.com/sis/apoptosis/docs/manual_apoptosis.pdf

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis, Cell Death, And Proliferation. Roche Manual. Includes a complete list of apoptosis methods. Roche. - [Read Apoptosis, Cell Death, And Proliferation. Roche Manual.]

Apoptosis: A Laboratory Manual of Experimental Methods - http://imoax1.unimo.it/~cossarizza/apobook98/index.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Apoptosis: A Laboratory Manual of Experimental Methods. Andrea Cossarizza & Diana Boraschi - [Read Apoptosis: A Laboratory Manual of Experimental Methods]

Arabidopsis Thaliana DNA Isolation Procedure - http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Procedure to isolate DNA from Arabidopsis Thaliana. - [Read Arabidopsis Thaliana DNA Isolation Procedure]

Arabidopsis thaliana DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdna.html

Category: Plant Biology Protocols: Arabidopsis Nucleic Acid Protocols

Protocol for Arabidopsis thaliana DNA isolation procedure. - [Read Arabidopsis thaliana DNA Isolation Protocol]

Arabidopsis Tissue Culture Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/tissue.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Arabidopsis Tissue Culture Stock Solutions and Media. Lazo Lab. MS MEDIUM FOR ARABIDOPSIS. B5 MEDIUM FOR ARABIDOPSIS. 10X Micronutrients. - [Read Arabidopsis Tissue Culture Stock Solutions and Media]

Arabidopsis transformation with pictures - http://www.bioinformatics.vg/Methods/arabitransformationf.htm

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Arabidopsis transformation and selection on kanamycin agar, Preparation of plants for transformation, Agrobacterium, Dipping, Post-dip care, Agar plates, Tips. Adaptation of the short protocol described bySteve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Arabidopsis transformation with pictures]

Assay of β-Galactosidase in Yeast Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/BetaGalAssays.pdf

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

There are two basic methods for the in vitro assay of B-galactosidase activity from yeast. They differ mainly in the method of preparing the material for assay. Both methods are described with accompanying protocols. Method I: Assay of Crude Extracts includes: Yeast Cell Growth; Yeast Cell Harvest; B-gal assays; Bradford Assays. Method II: Permeabilized cell assay. - [Read Assay of β-Galactosidase in Yeast Protocol]

Assays to Detect Apoptosis Protocol - http://www.promega.com/paguide/chap4.htm#title5

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol and methods on assays to detect apoptosis. - [Read Assays to Detect Apoptosis Protocol]

ß - GAL Filter Assay Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/b-GAL_filterassay.html

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for ß - GAL filter assay. - [Read ß - GAL Filter Assay Protocol]

B-GAL Filter Assay Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/b-GAL_filterassay.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Protocol for B-Gal filter assay. Includes Z buffer and X-Gal solution. - [Read B-GAL Filter Assay Protocol]

Bacterial clone DNA Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/DNAprep.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Bacterial clone DNA Protocol for FISH. - [Read Bacterial clone DNA Protocol for FISH]

Bacterial Solutions - http://www.uhmc.sunysb.edu/bioscience/methods/bacteria_methods/bacteria_methods.htm

Category: Buffers Media, and Solutions: Bacterial Solutions

Bacterial Solutions. Stony Brook Univ. Hospital. - [Read Bacterial Solutions]

Basic Methods of Culturing Drosophila Protocol - http://fly.bio.indiana.edu/Fly_Work/culturing.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for the basic methods of culturing Drosophila. Includes: Stockkeeping; Culture contaminants; Experimental populations. - [Read Basic Methods of Culturing Drosophila Protocol]

Biuret Protein Assay - http://homepages.gac.edu/~cellab/appds/appd-g.html#exG-3

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Biuret Protein Assay. Includes Materials, methods and procedure. - [Read Biuret Protein Assay]

Bradford Protein Assay - http://www.ruf.rice.edu/~bioslabs/methods/protein/bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

A detailed and well thought out Bradford protein assay page using a spectrophotometer. Includes information such as the Bradford assay is very fast and uses about the same amount of protein as the Lowry assay, comments, procedural steps, equipment used and more. - [Read Bradford Protein Assay]

Bradford Protein Concentration Assay - http://web.chemistry.gatech.edu/~williams/bCourse_Information/4581/techniques/bradford/bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Includes Abbreviations, Background, and Procedure steps using BSA. The Bradford protein assay (1) is one of several simple methods commonly used to determine the total protein concentration of a sample. The method is based on the proportional binding of the dye Coomassie to proteins. The assay is colorimetric; as the protein concentration increases, the color of the test sample becomes darker. Coomassie absorbs at 595 nm. - [Read Bradford Protein Concentration Assay]

Calcium Phosphate Transfection of Neurons in Primary Culture - http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Ca_P_TX_R.shtml

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Detailed Neuronal cell transfection protocol and methods. Steven Finkbeiner. UCSF. - [Read Calcium Phosphate Transfection of Neurons in Primary Culture]

Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3871

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

Calcium phosphate forms an insoluble precipitate with DNA, which attaches to the cell surface and is taken into the cells by endocytosis. The protocol is easily adapted for use with other types of cells, both adherent and nonadherent. This protocol is a modified version of a method published by Jordan et al. (1996) who rigorously optimized calcium-phosphate-based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney cells. - [Read Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs]

Caspase Detection Methods for Apoptosis - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/chap11.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Caspase Detection Methods for Apoptosis. Zeuner et al. Purdue Cytometry CD-ROM Series. - [Read Caspase Detection Methods for Apoptosis]

CAT Assay Protocol - http://kinase.uhnres.utoronto.ca/methods/CATassay.html

Category: Reporter Gene Assays: Cat Assays Protocols

Protocol for CAT assay. - [Read CAT Assay Protocol]

CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry - http://www.ihcworld.com/_protocols/antibody_protocols/cd90_pharmingen.htm

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for CD90 (Thy-1) antibody staining for Immunohistochemistry. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain. - [Read CD90 (Thy-1) Antibody Staining Protocol for Immunohistochemistry]

Cell Freezing using Liquid Nitrogen N2 - http://www.liv.ac.uk/~giles/methods/cell_freezing.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing gealthy cells (> 95% viable) the protocol obtains a culture 80-90% viable 24 hours after thawing and growing on test vial from step 9. Antisense Research Group - [Read Cell Freezing using Liquid Nitrogen N2]

Cell Lysate Extracts - http://www.upstate.com/misc/protocol_detail.q.prot.e.CellLysate_Prep.a.name.e.Cell_Lysate_Extracts-General_Protocols

Category: Protein Protocols: Protein Extraction From Cells

Cell Lysate Extracts. Great protocols for cell lysis preparation from a variety of cell types. There are numerous methods of cell stimulation and lysis. For a given protein, Upstateâ€™s Laboratories determine the specific treatment upon initial testing of its products. It is important to select the correct cell line, stimulation procedure (if any), and lysis protocol. Upstate. - [Read Cell Lysate Extracts]