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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid Protocol]

Triparental Mating Procedure for Xanthomonas (Recipient) Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/xctripar.html

Category: Bacteria Genetics Protocols

Protocol for triparental mating procedure for Xanthomonas (recipient). - [Read Triparental Mating Procedure for Xanthomonas (Recipient) Protocol]

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes - http://www.sanger.ac.uk/HGP/methods/cytogenetics/two_colour.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes. Sanger Institute - [Read Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes]

Understanding Protein Trafficking in Plant Cells Through Proteomics - http://www.cepceb.ucr.edu/resources/pdf/pan/ERP-review2_781_2005.pdf

Category: Protein Protocols: Protein Trafficking

Background and methods to study plant transport. Includes new methods to study protein trafficking in plant cells, includes: Identification of protein sorting pathways in non purified samples; Localization of organelle proteins by isotype tagging/isotype-coded affinity tag; Coupling of chemical genomics and proteomics; Top down mass spectrometry; Compartment-specific markers to aid in the purification of organelles. - [Read Understanding Protein Trafficking in Plant Cells Through Proteomics]

Unmasking Hidden Epitopes Using the Microwave Oven Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4529

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Several methods have been developed to "retrieve" antigens that have been masked by fixation. The principle behind using the microwave oven method described here is to use extended periods of heat to break some of the subcellular structures that block antibody access. Be aware that any of the antigen retrieval methods should be avoided wherever possible, because they may introduce artifactual false-positive staining. - [Read Unmasking Hidden Epitopes Using the Microwave Oven Protocol]

Vacuum-Infiltration Transformation of Arabidopsis thaliana Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/met3.html

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Protocol for vacuum-infiltration transformation of Arabidopsis thaliana. Includes: Plant Growth; Vacuum Infiltration; Selection of Putative Transformants; Kanamycin selection. - [Read Vacuum-Infiltration Transformation of Arabidopsis thaliana Protocol]

VEGF Antibody Staining Protocol - http://www.ihcworld.com/_protocols/antibody_protocols/vegf_vg1_chemicon.htm

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for VEGF antibody staining. Includes: Primary Antibody; Antigen Retrieval; Detection Methods; Chromogen Substrate; Counterstain. - [Read VEGF Antibody Staining Protocol]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]

Vogel's Minimal Media Protocol - http://www.fgsc.net/methods/vogels.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Vogel's 50X salts as given in the Microbial Genetics Bulletin 13:42-43, 1956. In 750 ml. distilled water, dissolve successively with stirring at room temperature. - [Read Vogel's Minimal Media Protocol]

Vogels Medium N (Minimal) Protocol - http://www.fgsc.net/methods/vogels.html

Category: Fungi Protocols

Protocol for Vogels medium N (minimal). - [Read Vogels Medium N (Minimal) Protocol]

Western Analysis Using the Chemiluminescent Alkaline Phosphatase Substrate CSPD Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4256

Category: Western Blot Protocols: Antigen Detection Methods Protocols

Use of the chemiluminescence-producing alkaline phosphatase substrate 3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo-[3.3.1.1(3,7)]decan]-4-yl)phenyl phosphate (AMPPD, also known as adamantyl-1,2-dioxetane phosphate), or its dioxetane relatives provides a substantial increase in sensitivity over colorimetric substrates and radiochemical methods currently used for the detection of antigen-antibody complexes immobilized on nylon or PVDF membranes. - [Read Western Analysis Using the Chemiluminescent Alkaline Phosphatase Substrate CSPD Protocol]

Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System - http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols

This protocol describes nuclear and cytoplasmic fractionation of tissue culture cells and a method for Western blot detection of proteins using the Odyssey Infrared Imaging System. This protocol was used to detect expression of the "small" Tap protein in 293T, HeLa and COS cells. The Odyssey system has several advantages over the more widely used chemiluminescent detection methods. - [Read Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System]

Whole Cell Patch Clamp Technique - http://www.iac-usnc.org/Methods/wholecell/index.html

Category: Electrophysiology Ion Channels

Whole Cell Patch Clamp Technique and Protocol. KAMRAN KHODAKHAH. Albert Einstein College of Medicine. - [Read Whole Cell Patch Clamp Technique]

Whole Cell Patch Clamp Technique - http://www.iac-usnc.org/Methods/wholecell/index.html

Category: Neuroscience Protocols: Patch Clamping Protocols

The technique of patch clamp recording was invented by Bert Sakmann and Erwin Neher in 1981 for which they received the NOBEL prize. The technique is best suited for the study of the behaviour of single ion channels, or macroscopic currents in small cells or macro-patches. The whole cell technique allows one to control the composition of solutes on both sides of the membrane. - [Read Whole Cell Patch Clamp Technique]

Working with Bacteriophage P1 and Its Cloning Systems Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4003

Category: Viral Manipulation Protocols: Phage Protocols

Protocol describes methods for recovery and purification of recombinant clones of bacteriophage P1 or PAC DNAs from bacteria. Because of their large size, these DNAs are sensitive to shearing forces and must be handled carefully. This protocol generally yields P1 DNA that works well as a substrate or template in enzymatic reactions. - [Read Working with Bacteriophage P1 and Its Cloning Systems Protocol]

Xenopus cDNA Libraries - http://www.xenbase.org/other/static/methods/cDNA_libraries.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Xenopus cDNA libraries. Includes: General cDNA clone providers. - [Read Xenopus cDNA Libraries]

Xenopus Chromatin immmunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/XChIP.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus chromatin immunoprecipitation. - [Read Xenopus Chromatin immmunoprecipitation Protocol]

Xenopus DNA Fishing Protocol - http://spot.colorado.edu/~klym/Methods/fishing.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus DNA fishing. Includes: DNA-agarose coupling reaction; PROTEIN BINDING REACTION; OLIGO ANNEALING. - [Read Xenopus DNA Fishing Protocol]

Xenopus Fluorescent in situs and FCIS Protocol - http://www.xenbase.org/other/static/methods/FISH.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal. - [Read Xenopus Fluorescent in situs and FCIS Protocol]

Xenopus Oocyte Transfer Protocol - http://www.xenbase.org/other/static/methods/anti-methods/as-proto.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus oocyte transfer. - [Read Xenopus Oocyte Transfer Protocol]

Xenopus RNA Isolation Protocol - http://spot.colorado.edu/~klym/Methods/RNAisolation.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus RNA isolation. - [Read Xenopus RNA Isolation Protocol]

Yeast Chromatin Immunoprecipitation (ChIP) - http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/hahnlab_ChIP_method.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Chromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab. - [Read Yeast Chromatin Immunoprecipitation (ChIP)]

Yeast Chromosomal DNA Prep Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/chromosomal-DNA.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast chromosomal DNA prep. - [Read Yeast Chromosomal DNA Prep Protocol]