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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Single Colour Fish Analysis Method - http://www.sanger.ac.uk/HGP/methods/cytogenetics/single_colour.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Single colour detection for biotinylated probes. Sanger Institute - [Read Single Colour Fish Analysis Method]

Slide preparation for FISH and Cytogenetics - http://www.sanger.ac.uk/HGP/methods/cytogenetics/slide_preparation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Slide preparation for FISH and Cytogenetics. - [Read Slide preparation for FISH and Cytogenetics]

Slide Preparation for Manual Microdissection Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/SlidePrepManualMicrodissection.html

Category: Primary Cell Isolation and Culture Protocols

Slide Preparation for Manual Microdissection for Subsequent DNA, RNA, and Protein Analysis. Manual microdissection and subsequent molecular analysis can be carried out on slides stained using standard hematoxylin and eosin methods. However, if cell types that are (or are not) expressing a specific protein are required for a study, then more advanced slide preparation methods such as Immuno-LCM may be utilized. - [Read Slide Preparation for Manual Microdissection Protocol]

Slow crystallization Protocol for MALDI samples - http://prowl.rockefeller.edu/methods/slowcrys.htm

Category: Mass Spectrometry Protocols

The dried-droplet method will fail for samples containing significant concentrations of involatile solvents or high concentrations of salts. PROWL - [Read Slow crystallization Protocol for MALDI samples]

Small Scale Plasmid Isolation for Xanthomonas - http://wheat.pw.usda.gov/~lazo/methods/lazo/xcmini.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol describing isolation of small scale plasmid from Xanthomonas. - [Read Small Scale Plasmid Isolation for Xanthomonas]

Sporulation and Dissection of Yeast a/alpha Diploids Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_sporulation.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for sporulation and dissection of yeast a/alpha diploids. - [Read Sporulation and Dissection of Yeast a/alpha Diploids Protocol]

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN - http://ylwang.umassmed.edu/protocol/cfs/dbp.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN. Indirect and direct methods. Yu li Wang labs. - [Read STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN]

Standard Ligation - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/molecularCloning/standardLigation.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

Nice guide for DNA ligation reaction. Standard Ligation for DNA. Schneitz Lab. - [Read Standard Ligation]

Sterilization and Filtration Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B9D0482A943EC97AC4807854493&objectid=6673A220D50910E56F81295AE515690F

Category: Cell Culture Protocols: Sterile Technique Protocols

This unit on sterility in the tissue culture environment describes methods for sterilization of liquid and dry goods used for tissue culture and filtration of liquids to prevent contamination of cultures. - [Read Sterilization and Filtration Protocol]

Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/stocksgl.html

Category: Buffers Media, and Solutions

Stock Solutions and Media. Gerard R. Lazo - [Read Stock Solutions and Media]

Storage of Sera Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4279

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the methods of storage for antibody-containing sera. Antibodies are resistant to a broad range of mildly denaturing conditions, so long-term storage is relatively easy. - [Read Storage of Sera Protocol]

Storage of Tissue Culture Supernatants Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4280

Category: Antibody Protocols: Antibody Handling Protocols

This protocol describes the methods of storage for hybridoma tissue-culture supernatants. - [Read Storage of Tissue Culture Supernatants Protocol]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cytogenetics Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Prtocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Prtocol]

Subcloning tips and methods. - http://www.its.caltech.edu/%7Ebjorker/Martin_subcloning%20procd.pdf

Category: DNA Protocols: DNA Cloning

Subcloning tips and methods. Very good for all cloning procedures. Bjorkman Group - [Read Subcloning tips and methods.]

Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues. - http://www.axis-shield.com/densityhome/optiprep/M17.pdf

Category: Cell Biology and Cell Culture

Summary of the techniques used for the purification of viruses using pre-formed iodixanol gradients. Viruses purified include: Semliki Forest, Retrovirus (from human melanoma cells), Rabies, Polyoma, Papillomavirus, Lassa, Influenza, Bovine viral diarrhea. - [Read Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues.]

TCA Protein concentration of Laemmli gel samples - http://kinase.uhnres.utoronto.ca/methods/TCA.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

TCA Protein concentration of Laemmli gel samples. Woodgett Lab. - [Read TCA Protein concentration of Laemmli gel samples]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Microscopy Protocols: In Vivo Imaging Protocols

Sophisticated fluorescence microscopy methods & equipment, now allow cellular events to be studied at high resolution in living material. The studying of living fly tissues presents unique difficulties in keeping the cells alive, introducing fluorescent probes, & imaging through thick hazy cytoplasm. This protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Drosophila Protocols

Protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material Protocol]

Tissue Culture Methods - http://www.research.umbc.edu/~jwolf/method5.htm#tc%20methods

Category: Cell Biology and Cell Culture: Cell Culture Techniques

Tissue Culture Methods. Julie B. Wolf, Univ. Maryland. Protocols for Counting Cells, Preservation and storage of cell lines, maintenance of cell lines, media and growth, and safety guidelines for working with cells. - [Read Tissue Culture Methods]

Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomic - http://www.nature.com/nprot/journal/v1/n4/abs/nprot.2006.273.html

Category: Protein Protocols: Protein Extraction From Tissue

Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics. Although many methods exist for fractionating proteins, the method described here can capture the majority of subcellular fractions simultaneously at reasonable purity. The scalability of this method makes it amenable to small samples, such as embryonic tissues, in addition to larger tissues. The protocol described is for the general fractionation and extraction of proteins from organs / tissue - [Read Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomic]

Titration of DNaseI for nick translation - http://www.sanger.ac.uk/HGP/methods/cytogenetics/dnase.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Titration of DNaseI for nick translation. Sanger Institute. - [Read Titration of DNaseI for nick translation]

Toxicity of Antibiotics and Other Drugs to Neurospora - http://www.fgsc.net/methods/fgn21tox.html

Category: Fungi Protocols: Neurospora Protocols

Information and tips on the toxicity of antibiotics and other drugs to Neurospora. - [Read Toxicity of Antibiotics and Other Drugs to Neurospora]

Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. The calcium phosphate method can be used to transfect neurons that have already been growing on coverslips in vitro. Transfected cells suitable for imaging can be obtained in cultures up to 15 days in vitro. - [Read Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid]