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Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Rapid Method for Preparation of Genomic DNA from P. aeruginosa Protocol - http://wheat.pw.usda.gov/~lazo/methods/goldberg/genomic.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for rapid method for preparation of genomic DNA from P. aeruginosa. - [Read Rapid Method for Preparation of Genomic DNA from P. aeruginosa Protocol]

Real-Time PCR (qPCR) Basics - http://web.archive.org/web/20040711051207/http://primerdesign.co.uk/primerdesign/qPCR_basics.html

Category: Real Time PCR

Learn Quantitative PCR Methodology. Assay parameters, Melt Curves and Primer Dimers, Standard Curves, Dynamic Range, Quantification methods, Delta CT, Standard Curves. Dr.Powell. - [Read Real-Time PCR (qPCR) Basics]

Real-Time RT-PCR: cDNA Synthesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4114

Category: PCR Protocols: Real-Time PCR Protocols

Protocol uses the Superscript II First-Strand Synthesis system for the generation of cDNA from total RNA. RNA purified using TRIzol reagent (Invitrogen) or the methods described in Preparation of RNA Using Guanidinium Isothiocyanate/Cesium Chloride Ultracentrifugation, Preparation of RNA from Paraffin-Embedded Fixed Tissue. - [Read Real-Time RT-PCR: cDNA Synthesis Protocol]

Real-Time RT-PCR: cDNA Synthesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4114

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

Recovery of LPS for SDS-PAGE Protocol - http://wheat.pw.usda.gov/~lazo/methods/goldberg/lps-reco.html

Category: Bacterial Protocols

Protocol for the recovery of LPS for SDS-PAGE. - [Read Recovery of LPS for SDS-PAGE Protocol]

Restraint and Handling of Animals Guide - http://www.ahc.umn.edu/rar/handling.html

Category: Animal Research Techniques

Handling Methods for animals: mice, rats, hamsters, rabbits, cats, dogs, non-human primates, swine, goats, sheep, calves, pigs. Restraint devices, drug administration. - [Read Restraint and Handling of Animals Guide]

Restriction Analysis of DNA Methylation Protocol - http://www.methods.info/Methods/DNA_methylation/methylation_restr.pdf

Category: Nucleic Acid Modification Protocols: DNA Methylation Protocols

Protocol for restriction analysis of DNA methylation. - [Read Restriction Analysis of DNA Methylation Protocol]

Restriction Digestion Methods - http://hg.wustl.edu/hdk_lab_manual/rdm/rdmcontents.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Digestion Methods. Donis-Kellor lab manual. Restriction Enzyme Digests, Restriction Enzyme Buffers, Restriction Digestion of Plasmid, Cosmid, and Phage DNAs. - [Read Restriction Digestion Methods]

Restriction Endonuclease Digestion of DNA - http://www.cf.ac.uk/biosi/staff/kille/Methods/Gene/Restriction_Digests.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Endonuclease Digestion of DNA. Double and Single Digestions. Cardiff Univ. PK group - [Read Restriction Endonuclease Digestion of DNA]

Reverse Ligation Mediated RT - PCR - http://www.liv.ac.uk/~giles/methods/rlpcr.htm

Category: RNA Protocols: RT-PCR and cDNA synthesis

This procedure was first described by Bertrand et al to demonstrate that ribozymes could be enzymatically active in vivo. We adapted the method to show that certain oligodeoxynucleotides could direct the activity of endogenous ribonuclease H to cleave tar - [Read Reverse Ligation Mediated RT - PCR]

Reverse Transcriptase-PCR Lazo Lab - http://wheat.pw.usda.gov/~lazo/methods/lazo/revtrpcr.html

Category: RNA Protocols: RT-PCR and cDNA synthesis

Reverse Transcriptase-PCR Lazo Lab/Jim Hutchins - [Read Reverse Transcriptase-PCR Lazo Lab]

RNA Affinity Isolations with PUMILIO from Drosophila melanogaster Protocol - http://www.pharma.ethz.ch/institute_groups/pharmacogenomics/research_gerber/methods/RNA_affinity_Drosophila_method.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for RNA affinity isolation with PUMILIO from Drosophila melanogaster. - [Read RNA Affinity Isolations with PUMILIO from Drosophila melanogaster Protocol]

RNA Affinity Isolations with PUMILIO from Drosophila melanogaster Protocol - http://www.pharma.ethz.ch/institute_groups/pharmacogenomics/research_gerber/methods/RNA_affinity_Drosophila_method.pdf

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols

Protocol for RNA affinity isolations with PUMILIO from Drosophila melanogaster. - [Read RNA Affinity Isolations with PUMILIO from Drosophila melanogaster Protocol]

RNA co-immunopurification with specific RNA-binding proteins - http://www.pharma.ethz.ch/institute_groups/pharmacogenomics/research_gerber/methods/RNA-IP_method_yeast_0805.pdf

Category: Immunology: Immunoprecipitation Protocols

Protocol for RNA co-immunopurification with specific RNA-binding proteins. Includes: Binding to IgG beads; Washing the beads; Elution with TEV-protease; RNA isolation. - [Read RNA co-immunopurification with specific RNA-binding proteins]

Rotor equilibration without counterbalance - http://www.methods.info/Tips/general_lab/rotor_equilibration.html

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Dr.A.Gratchev - [Read Rotor equilibration without counterbalance]

RT-PCR Protocol and PCR - http://www.bioinformatics.vg/Methods/RT-PCR_protocol.shtml

Category: RNA Protocols: RT-PCR and cDNA synthesis

RT-PCR Protocol and PCR. Tips and Method. - [Read RT-PCR Protocol and PCR]

S20 Purification of detergent-insoluble lipid rafts from cells and tissues. - http://www.axis-shield.com/densityhome/optiprep/S20.pdf

Category: Cell Biology and Cell Culture

Protocol for the isolation of the lipid-rich microdomains of the plasma membrane, notably caveolae and lipid rafts. Methods for the isolation of lipid rafts are based on the insolubility of these structures in the nonionic detergent TritonX-100. Either the intact cells are treated with a detergent-containing solution or a post-nuclear supernatant is prepared from a cell homogenate and then Triton X-100 is added to this supernatant. - [Read S20 Purification of detergent-insoluble lipid rafts from cells and tissues.]

SAPK / Jun kinase assays - http://kinase.uhnres.utoronto.ca/methods/SAPKassays.html

Category: Signalling Signal Transduction Protocols

SAPK/Jun kinase assays. Preparation of cell lysate, Substrate/Antibody, Lysate Interaction and buffer recipes. Woodgett Lab PMH. - [Read SAPK / Jun kinase assays]

SAPK/Jun Kinase Assays - http://kinase.uhnres.utoronto.ca/methods/SAPKassays.html

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol for SAPK/Jun kinase assays. Protocol includes information on: preparation of cell lysate, substrate/antibody, lysate interaction. - [Read SAPK/Jun Kinase Assays]

Screening cDNA Libraries Constructed in Eukaryotic Expression Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4069

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Two methods of screening pools of cloned cDNAs are discussed: transfection into mammalian cells and injection into Xenopus oocytes. - [Read Screening cDNA Libraries Constructed in Eukaryotic Expression Vectors Protocol]

SDS-PAGE Gels - http://mycoplasmas.vm.iastate.edu/lab_site/methods/protein/SDS-PAGE.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE Gels. Adapted from David Bowtell - [Read SDS-PAGE Gels]

Sectioning Mouse Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4703

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods and tips for sectioning mouse embryos and transferring the sections to a microscope slide. - [Read Sectioning Mouse Embryos Protocol]

Seeded films (Vorm-Roepstorff) MALDI Protocol - http://prowl.rockefeller.edu/methods/seeded.htm

Category: Mass Spectrometry Protocols

This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sample stageâ€™s surface. MALDI Sample preparation. PROWL - [Read Seeded films (Vorm-Roepstorff) MALDI Protocol]

Short-Term Storage of Tetrahymena Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4470

Category: Tetrahymena Protozoa Protocols

A number of methods can be used for storage of unfrozen Tetrahymena cultures in the laboratory. Cells that are maintained using the short-term storage are described in this protocol. - [Read Short-Term Storage of Tetrahymena Cultures Protocol]

Singel Nucleotide Primer Extension (SNuPE) - http://www.methods.info/Methods/DNA_methylation/SNuPE.html

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Single Nucleotide Primer Extension can be used for the analysis of methylation in a certain position. Treat DNA with bisulphite and then anneal a primer which ends immediately before the site of analysis. Dr. A. Gratchev Methods.info - [Read Singel Nucleotide Primer Extension (SNuPE)]