methanol Protocols

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C. elegans Gonad Fixation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_fixation.html

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for C.elegans gonad fixation. Includes fixation with: Methanol, Formaldehyde, Methanol / Formadehyde, Glutaraldehyde / Formaldehyde. - [Read C. elegans Gonad Fixation Protocol]

Cell Fixation Using Methanol - http://www.lamondlab.com/pdf/CellFixMEOH.pdf

Category: Immunology: Immunocytochemistry Protocols

Methanol Fixation for Immunofluorescence. Lamond Lab, Univ. Dundee - [Read Cell Fixation Using Methanol]

Cell Staining for Immunofluorescence Microscopy - http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Cell Staining for Immunofluorescence Microscopy. Includes protocols for fixing the cells, Coverslip Preparation for Adherent Cells, Coverslip Preparation for Non-Adherent Cells, Paraformaldehyde Fixation, and Methanol/Acetone Fixation. Blocking protocols include blocking with primary antibody, and incubation with secondary antibody. - [Read Cell Staining for Immunofluorescence Microscopy]

Extraction of Calf Brain Lipids Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1281

Category: Lipid Protocols

Protocol for the extraction of calf brain lipids. Protocol describes a rapid method to isolate lipids from bovine brain tissue using an organic solvent mixture of Chloroform and Methanol. - [Read Extraction of Calf Brain Lipids Protocol]

Fixation of Mouse Embryos and Tissues Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4702

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution. - [Read Fixation of Mouse Embryos and Tissues Protocol]

Fixing Attached Cells in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4294

Category: Cell Culture Protocols: Cell Fixing Protocols

Treating cells with paraformaldehyde leads to the establishment of chemical cross-links between free amino groups. When the cross-links join different molecules, a latticework of interactions occurs that holds the overall architecture of the cell together. Commercial formaldehyde solutions are not recommended, because they lack the advantages of using a variable-length polymer, and the cells will simultaneously be fixed with the alcohol (usually methanol). - [Read Fixing Attached Cells in Paraformaldehyde Protocol]

Methanol-Chloroform Precipitation of Proteins - http://www.healthsystem.virginia.edu/internet/biomolec/methanolchloroform.cfm

Category: Mass Spectrometry Protocols

Methanol-Chloroform Precipitation of Proteins. A procedure for precipitating proteins from solution, including detergent solutions, is from Wessel and FlÃ¼gge (1984). Univ. Virginia. - [Read Methanol-Chloroform Precipitation of Proteins]

Protein Precipitation Protocols - http://www.ls.huji.ac.il/~purification/Protocols/ProteinPrecipitation.html

Category: Protein Protocols: Protein Precipitation Procols

TCA-DOC Normal, TCA Acetone Precipitation, Ethanol Precipitation, TCA-DOC / Acetone, Acidified Acetone/Methanol, Dr. Mario Lebendiker. University of Jerusalem. - [Read Protein Precipitation Protocols]

Protocol for Microtubule Binding Assays - http://www.proweb.org/kinesin/Methods/MT-binding_assay.html

Category: Cell Biology and Cell Culture

Microtubule binding assay protocol using the materials: Siliconized ultracentrifuge microfuge tubes, GTP-depleted microtubules, 6X SDS loading dye, 1X SDS loading dye, Coomassie Brilliant Blue R 250 (0.8% in 50% methanol + 10% acetic acid)and Destaining solution (13% methanol + 3% acetic acid). - [Read Protocol for Microtubule Binding Assays]

Yeast Immunofluorescence with methanol - http://www.upstate.edu/biochem/amberg/protocols/MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF with Methanol/Acetone Dehydration. Amberg Lab, Upstate Medical University. - [Read Yeast Immunofluorescence with methanol]

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Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.