metabolism Protocols

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes the use of SEMV cells (a cell line derived from ram seminal vesicles) in studies into prostaglandin H synthase-mediated metabolism of xenobiotics in intact cells. - [Read In Vitro Model For Studies Of Prostagland H Synthase (PHS)- Mediated Genotoxicity Of Xenobiotics]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Protocol describes the use of SEMV cells (a cell line derived from ram seminal vesicles) in studies into prostaglandin H synthase-mediated metabolism of xenobiotics in intact cells. - [Read Invitro Model for Prostoglandin H Synthase (PHS) Mediated Genotoxicity of Xenobiotics]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

The basis of this procedure is that two specific cell type preparations may be isolated, exposed separately to various compounds over a range of concentrations, and the cytotoxicity of these determined.  Parameters deemed indicative of a cytotoxic effect include a reduction in de novo protein synthesis and decreased glucose and fatty acid metabolism.  A cytotoxic effect may indicate that a chemical is likely to be nephrotoxic in vivo. - [Read Isolated Rat Glomeruli and Proximal Tubules]

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Theory, Galactose metabolism, The Pros and Cons of the Two hybrid Assay. Troubleshooting and Tips for Hybrid assay. Sobhanifar BioTeach Journal 2003. - [Read Yeast Two Hybrid Assay: A Fishing Tale]