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Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols
Protocol utilizes the Y190 yeast strain, that characteristically turns from white to red during growth due to ademutation. Baits were subcloned into the pAS1cyh2 vector, and Preys subcloned into pACT2. - [Read Directed Yeast Two Hybrid Screening for MEKK1 Interaction Partners Protocol]
Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols
Protocol for immunoprecipitation of endogenous MEKK1 and kinase assay. Includes: Preparation of lysates; Immunoprecipitation; Kinase assay. - [Read Immunoprecipitation of Endogenous MEKK1 and Kinase Assay Protocol]
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