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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

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Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Yeast Protocols

Plasma membranes are isolated from the yeast Saccharomyces cerevisiae. The cell wall is initially digested by helicase, followed by hypoosmotic lysis and homogenization. Membranes are prepared by subsequent differential centrifugation. The activity of the H+-ATPase is then determined by measuring the amount of inorganic phosphate released from ATP. - [Read Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol]

Yeast Plasma Membrane H+ -ATPASE Toxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

Accumulation of lipophilic substances, many of which may be environmental chemicals, affects the membrane lipid order and consequently affects the functions of these proteins. Since, the function of important cellular proteins, such as the H+-ATPase strongly depends upon the integrity of the lipid bilayer, the activity of the H+-ATPase may be used as a sensitive indicator of the effect that a chemical may have on the viability of the cell. - [Read Yeast Plasma Membrane H+ -ATPASE Toxicity Test]

Yeast Plates Protocols - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_plates.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast plates protocols. Includes: Glucose Complete Plates (1 liter); YPD Plates (1 liter); 5-FOA plates (0.5 liter). - [Read Yeast Plates Protocols]

Yeast Protein Extracts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4152

Category: Yeast Protocols: Yeast Protein Protocols

Protocol is a simple, reliable method for the preparation of yeast protein extracts for analysis by polyacrylamide gel electrophoresis (PAGE) and Western blotting. - [Read Yeast Protein Extracts Protocol]

Yeast RNA Isolation: Large-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4154

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes large-scale yeast RNA isolation. It is designed to yield more than 10 mg of total nucleic acid and 300-400 µg of poly(A)-selected mRNA from each 500-ml culture. - [Read Yeast RNA Isolation: Large-Scale Protocol]

Yeast RNA Isolation: Small-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4155

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes rapid, small-scale yeast RNA isolation. It is based on the work of Schmitt et al. (1990). Note that all containers should be washed in Rnase Away (Invitrogen) or dry baked for 24 hours at 160°C. - [Read Yeast RNA Isolation: Small-Scale Protocol]

Yeast Transformation (for LexA Library Screen) Protocol - http://www.natureprotocols.com/2006/12/15/yeast_transformation_for_lexa.php

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for Yeast transformation (for LexA Library Screen). - [Read Yeast Transformation (for LexA Library Screen) Protocol]

Yeast Transformation (high efficiency) Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/dmso_yeast_transform.html

Category: Yeast Protocols: Yeast Transformation Protocols: High Efficiency Yeast Transformation Protocols

Protocol for high efficiency Yeast transformation. - [Read Yeast Transformation (high efficiency) Protocol]

Yeast Transformation Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/yeast-trafo.html

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for Yeast transformation. - [Read Yeast Transformation Protocol]

Yeast Two Hybrid Assay: A Fishing Tale - http://www.bioteach.ubc.ca/Journal/V01I01/8188yeast.pdf

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Theory, Galactose metabolism, The Pros and Cons of the Two hybrid Assay. Troubleshooting and Tips for Hybrid assay. Sobhanifar BioTeach Journal 2003. - [Read Yeast Two Hybrid Assay: A Fishing Tale]

Yeast Two-Hybrid Screen with Library and Bait - http://faculty.virginia.edu/mirmira/resources_files/Protocols/YEAST%20TWO.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia. - [Read Yeast Two-Hybrid Screen with Library and Bait]

Yeast Two-hybrid Systems Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3889

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

Protocol for Yeast two-hybrid systems. - [Read Yeast Two-hybrid Systems Protocol]

Yeast Two-Hybrid: State of the Art - http://www.biologicalprocedures.com/bpo/arts/1/16/m16.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Yeast Two-Hybrid: State of the Art. Paper on Protein-protein interactions and the two-hybrid protocol. Wim Van Criekinge et al., BPO. - [Read Yeast Two-Hybrid: State of the Art]

Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4166

Category: Yeast Protocols: Yeast Staining Protocols

Various vital stains are available for yeast. This protocol describes the staining of chitin and bud. - [Read Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol]

Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4163

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes the staining of nuclear and mitochondrial DNA with DAPI (4',6-diamidino-2-phenylindole). - [Read Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol]

Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4164

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes the visualization of mitochondria with DiOC6 or DiIC5(3). - [Read Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol]

YPD Agar Protocol - http://www.thelabrat.com/protocols/YPDAgar.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Agar Cell Culture Media Protocols

Protocol for YPD Agar. - [Read YPD Agar Protocol]

YPD Media Protocol - http://www.thelabrat.com/protocols/YPDmedia.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for YPD media. - [Read YPD Media Protocol]

YPG Media Protocol - http://www.thelabrat.com/protocols/YPGmedia.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for YPG media. - [Read YPG Media Protocol]

Zeiss Meta Confocal Microscopy Imaging Instructions - http://depts.washington.edu/keck/Zeiss/Zeiss%20Instructions.html

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Zeiss Meta Confocal Microscopy Imaging Instructions. Operating Instructions with photos included. Keck Imaging Center. - [Read Zeiss Meta Confocal Microscopy Imaging Instructions]

Zero Blunt TOPO PCR Cloning Kit Protocol - http://www.hawaii.edu/microbiology/MO/topo.htm

Category: DNA Protocols: TOPO Cloning

Zero Blunt TOPO PCR Cloning Kit, Detailed protocol. (Invitrogen Life technologies). Alam Lab. Department of Microbiology, University of Hawai'i. - [Read Zero Blunt TOPO PCR Cloning Kit Protocol]

Zinc Imidazole Negative Staining Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/StainingProcedures.html#Zinc

Category: Protein Staining Protocols

Protocol for zinc imidazole negative staining. - [Read Zinc Imidazole Negative Staining Protocol]

[3H]Thymidine Labeling Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/T3.html

Category: Cell Culture Protocols

Protocol on [3H]Thymidine labeling. Protocol includes: Explant Culture Medium; 100X (cold) Thymidine; Dektol Developer; Fixer - [Read [3H]Thymidine Labeling Protocol]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.