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Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4445

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol describes transfection of plasmid DNA into primary hippocampal neurons using DNA/calcium-phosphate (CaPO4) coprecipitation. The precise pH of the transfection medium and the incubation time of cells with the coprecipitate are critical for reproducible and efficient transfection. Once these parameters are optimized for a given plasmid, the method is easily adapted for transfection of other established cell lines. - [Read Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation]

TransWell Chemotaxis Protocol. - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2156

Category: Immunology: Chemotaxis

TransWell Chemotaxis protocol. trans-well chemotaxis method from bioprotocol. The protocol is a method for studying migration towards a concentration gradient of chemoattractant of leukocytes (neutrophils, monocytes and lymphocytes) or other migratory cells. An upper chamber containing a suspension of cells is separated by a membrane from a lower chamber containing medium with chemoattractant. Chemotaxis of the cells from the upper chamber into the lower chamber can be quantified. - [Read TransWell Chemotaxis Protocol.]

Treatment of Cells with 5-aza-dC. protocol PDF - http://www.shmu.edu.cn/courses/2005aut/upload/20051116/Hongmei%20Xu%202003%20CANCER%20RESEARCH%20%20Aberrant%20Methylation%20and%20Silencing%20of%20ARHI,%20an%20Imprinted%20Tumor%20Suppressor%20Gene%20in%20which%20the%20Function%20Is%20Lost%20in%20Breast%

Category: Epigenetics and Methylation Protocols: 5-Aza-dC Treatment AZA Procedure

"Cells were seeded at a density of 1X106 cells/100-mm dish with 10% FBS and allowed to attach over a 24-h period. 5-Aza-dC (Sigma) was then added to a final concentration of 0.2â€“1 M, and the cells were allowed to grow for 5 days. The medium with or w - [Read Treatment of Cells with 5-aza-dC. protocol PDF]

Vogels Medium N (Minimal) Protocol - http://www.fgsc.net/methods/vogels.html

Category: Fungi Protocols

Protocol for Vogels medium N (minimal). - [Read Vogels Medium N (Minimal) Protocol]

Wet-lab protocols - http://eagle.mmid.med.ualberta.ca/private/labnotes/wetlab/index.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

LB plates, LB medium (for 1 liter), Agarose gels. Bart.Hazes Univ Alberta. - [Read Wet-lab protocols]

Yeast Media, Solutions and Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast11.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast Media, Solutions and Stocks Protocols. Includes: Yeast Media; AHC Medium; AHC plates; SD Medium; SD plates; Sorbitol-Agar Medium; Sorbitol-Agar Medium Top Agar; Sporulation Medium Plates; YPD Medium; YPD plates; Synthetic Media Supplements. - [Read Yeast Media, Solutions and Stocks Protocols]

[3H]Thymidine Labeling Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/T3.html

Category: Cell Culture Protocols

Protocol on [3H]Thymidine labeling. Protocol includes: Explant Culture Medium; 100X (cold) Thymidine; Dektol Developer; Fixer - [Read [3H]Thymidine Labeling Protocol]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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