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Drosophila Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/neurofood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila sucrose and yeast medium. - [Read Drosophila Sucrose and Yeast Medium Protocol]

Estimation of Cell Number by Hemocytometry Counting Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4454

Category: Cell Culture Protocols

Protocol describes a method for estimation of mammalian cell number in a defined volume of medium using a hemocytometer. Automated methods using cell-counting devices such as those produced by Coulter are desirable when large numbers of individual samples are to be counted. - [Read Estimation of Cell Number by Hemocytometry Counting Protocol]

Extraction and Solubilization of Total Protein from Microorganisms Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4224

Category: Bacterial Cell Culture Protocols

The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. - [Read Extraction and Solubilization of Total Protein from Microorganisms Protocol]

Fission Yeast Media Protocol - http://www-rcf.usc.edu/~forsburg/media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for fission yeast media. Includes: YES (yeast extract with supplements) for rich complete media; YSO, for modified rich media; EMM (Edinburgh minimal medium) for minimal selective media; stock solutions to make up minimal media; MB, a very stringent minimal for transformation; Sporulation media; ME (malt extract); SPAS; EMMGlut. - [Read Fission Yeast Media Protocol]

Flow Cytometry Analysis Protocol - http://cbr.med.harvard.edu/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Flow Cytometry Analysis Protocol. Springer Lab - Harvard. Background on flow cytometry technique: Flow cytometry is a method which uses instrumentation scanning single cells flowing past excitation sources in a liquid medium.
- [Read Flow Cytometry Analysis Protocol]

Fluorescence Mounting Medium (Antifade) - http://spectorlab.cshl.edu/fluorescence_medium.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorescence Mounting Medium (Antifade). Spector Lab, CSHL. - [Read Fluorescence Mounting Medium (Antifade)]

Fluorescence Mounting Medium (Antifade) Protocol - http://spectorlab.cshl.edu/fluorescence_medium.html

Category: Immunology: Immunofluorescence Protocols

Protocol for fluorescence mounting medium (antifade). - [Read Fluorescence Mounting Medium (Antifade) Protocol]

Human Embryonic Stem Cell Protocols - http://stemcells.nih.gov/staticresources/research/protocols/BresaGen_hESC_manual_2.1.pdf

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Human embryonic stem cells (hESCs) are pluripotent cells capable of differentiation to representatives of all three germ layers. Includes: Isolation of Primary Mouse Embryo Fibroblasts; Thawing and preparing p1 MEF feeder plates; Preparation of MEF- Conditioned Medium (MEF-CM; Microdissection Passaging of hESCs; Bulk passaging of hESC; Cryopreservation of hESCs; Thawing of hESCs; Karyotyping. - [Read Human Embryonic Stem Cell Protocols]

Human Lymphocyte Cytotoxicity Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/6.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

This method measures the leakage of DNA and lactate dehydrogenase from lymphocytes into the surrounding medium as an indicator of cytotoxicity. This method also includes an assay of intracellular (mitochondrial) diaphorase as a measure of cellular activity (MTT assay). - [Read Human Lymphocyte Cytotoxicity Assay Protocol]

Human Thyroid Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/29.htm#TOP

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This method enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. - [Read Human Thyroid Culture Protocol]

Human Thyroid Culture Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/29.htm#TOP

Category: Pharmacology and Toxicology Protocols: Drug Discovery

Protocol enables the culturing of thyroid cells without loss of differentiation and medium change. It is potentially useful for the long-term study of drug effects on the thyroid gland. - [Read Human Thyroid Culture Protocol]

IPL-41 Insect Medium Protocol - http://www.sigmaaldrich.com/img/assets/11020/IPL41_Insect_Medium.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Insect Cell Culture Growth Media Protocols

Protocol for IPL-41 insect medium. - [Read IPL-41 Insect Medium Protocol]

Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4410

Category: Stem Cell Protocols: Stem Cell Isolation Protocols

This protocol describes a method for isolating individual ES cell colonies by picking after they have grown on selective medium. - [Read Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking Protocol]

Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=572&criteria=

Category: Immunology: Mononuclear Cell Isolation Protocols

Mononuclear phagocyte progenitor cells derived from femoral and tibial bone marrow are propagated in the presence of M-CSF. This macrophage growth factor is secreted by L929 cells and is used in the form of L929 cell conditioned medium. The progenitor cells proliferate and differentiate through monoblast, promonocyte and monocyte stages before maturing to macrophages. At this time the cells become firmly adherent to the culture vessel. - [Read Isolation and Culture of Mouse Bone Marrow-Derived Macrophages Protocol]

Isolation of Primary Epithelial Cells - http://www.lbl.gov/lifesciences/BissellLab/labprotocols/primarycellprep.htm

Category: Cell Biology and Cell Culture: Epithelial Cell Culture

Isolation of Primary Epithelial Cells. Digestion: In Collagenase Medium, Purification and epithelial cell culture methods. Bissell Lab. - [Read Isolation of Primary Epithelial Cells]

Kanamycin Selection of Transformed Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4669

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

The most commonly used markers for selection of transgenic Arabidopsis are resistance to the antibiotic kanamycin and to the herbicide glufosinate ammonium. Resistance to kanamycin is conferred by a bacterial gene encoding the enzyme neomycin phosphotransferase (NPT). In this protocol, kanamycin-resistant seedlings are selected on solid medium. - [Read Kanamycin Selection of Transformed Arabidopsis Protocol]

Large-Scale Yeast Transformation Protocol - http://ygac.med.yale.edu/mtn/LS_transf_prot.stm

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for large-scale yeast transformation. Includes: Yeast Cell Preparation; Large Scale Transformation; To plate on solid medium; To select in liquid. - [Read Large-Scale Yeast Transformation Protocol]

Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4472

Category: Tetrahymena Protozoa Protocols

Long-term storage of unfrozen Tetrahymena is convenient when a laboratory is not using the cells frequently; however, cells maintained under these conditions often take a couple of days of incubation in culture medium to recover. - [Read Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium Protocol]

Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4471

Category: Tetrahymena Protozoa Protocols

Protocol provides a method for long-term storage of Tetrahymena cultures using maintenance culture medium. - [Read Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium Protocol]

Lymphocyte Isolation and Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4480

Category: Immunology: Mononuclear Cell Isolation Protocols: B Lymphocyte Isolation Protocols

Protocol describes a method for isolation and stimulation of lymphocytes. Leukocyte-rich plasma is collected from whole blood and then centrifuged through Ficoll-Hypaque. The collected cells are resuspended in growth medium containing various mitogens to stimulate growth. - [Read Lymphocyte Isolation and Culture Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for media for culture of Aspergillus nidulans. Includes: Minimal medium; Complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Mammalian Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B6F09C1BB8FBC47FC2A7E61A8A3&objectid=66739B890A55AC13354418E67F28E693

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This protocol provides guidelines for design of serum-containing and serum-free media, selective and specialty media, and media for growth under special conditions such as soft-agar growth. - [Read Media for Culture of Mammalian Cells Protocol]

Metabolic Labeling & Immunoprecipitation Protocols - http://www.cbrinstitute.org/labs/springer/protocols/jun_35Slabeling_IP.html

Category: Immunology: Immunoprecipitation Protocols: Immunoprecipitation of Radiolabeled Cells Protocols

Cells incorporate 35S-methionine or cysteine during the protein synthesis. Thus it is essential to use Met,Cys-free medium and dialyzed FCS during the labeling. Short period of incubation with 35S-methionine or cysteine will result in radiolabeling (pulse), and additional incubation with excess concentration of unlabeled Met+Cys (chase) is needed for complex glycoproteins like integrins to get expressed as a maturated form. - [Read Metabolic Labeling & Immunoprecipitation Protocols]

Minimum Essential Medium Eagle (MEM) Protocol - http://www.sigmaaldrich.com/img/assets/11020/MEM.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for minimum essential medium eagle(MEM). - [Read Minimum Essential Medium Eagle (MEM) Protocol]

Mounting Samples in DPX Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4460

Category: Cell Culture Protocols: Cell Fixing Protocols

Mounting media for immunohistology must be compatible with the detection method used. A suitable non-aqueous mounting medium is DPX. - [Read Mounting Samples in DPX Protocol]

Articles (1-4 of 4)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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