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A semi-permanent mounting medium for immunofluorescence microscopy - http://www.hei.org/research/aemi/moviol.htm

Category: Molecular Imaging: Light Microscopy

A semi-permanent mounting medium for immunofluorescence microscopy. House Ear Institute - [Read A semi-permanent mounting medium for immunofluorescence microscopy]

Adaptation of High Five™ Cells to HyQ© SFX Insect Medium - http://www.hyclone.com/pdf/procedure_insect_highfive.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

This protocol is designed to adapt High Five™ (BTI-Tn-4) cells to serum-free suspension culture in HyQ SFX-Insect in 5 passages. The entire adaptation process takes approximately two weeks and the resulting adapted cells have doubling times of 16 and 20 hours and minimal clumping in suspension culture. - [Read Adaptation of High Five™ Cells to HyQ© SFX Insect Medium]

Adapting Cells to a Serum-Free Environment Protocol - https://fscimage.fishersci.com/webimages_FSC/downloads/HyClone_Protocol_8.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

There are many ways to adapt cell lines to serum-free media. Five methods are presented that are designed for adapting hybridomas to a protein-free medium. These protocols may require some modifications for your particular cell line and conditions. - [Read Adapting Cells to a Serum-Free Environment Protocol]

Amplification and Storage of a Cosmid Library: Amplification in Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4001

Category: DNA Protocols: Cosmid Library Protocols

Amplification of cosmid libraries may result in distorted representation of cloned genomic sequences and should be avoided wherever possible. If amplification should become necessary, it is best to expand the library by growth in liquid medium. - [Read Amplification and Storage of a Cosmid Library: Amplification in Liquid Culture Protocol]

Analysis of Membrane Trafficking and Intracellular Signaling in Self-Generated Iodixanol Gradients - http://www.axis-shield.com/densityhome/optiprep/S25.pdf

Category: Protein Protocols: Protein Trafficking

Protocol is based on methods for the resolution of GLUT4 containing vesicles and the identification of phosphoinositide kinase containing vesicles in 3T3-L1 adipocytes. They may have a wider application to any low-medium density membranes. Protocol incorporates the strategy of using a low density microsome fraction as the gradient input, commonly used in GLUT 4 studies that may have a wider application to other investigations. - [Read Analysis of Membrane Trafficking and Intracellular Signaling in Self-Generated Iodixanol Gradients]

Arabidopsis Tissue Culture Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/tissue.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Arabidopsis Tissue Culture Stock Solutions and Media. Lazo Lab. MS MEDIUM FOR ARABIDOPSIS. B5 MEDIUM FOR ARABIDOPSIS. 10X Micronutrients. - [Read Arabidopsis Tissue Culture Stock Solutions and Media]

Basic Techniques for Mammalian Cell Tissue Culture Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B5FE053DEE8CB4AC3EB6F729A11&objectid=667396C6F0D96C40EB68A3D8DB8B201A

Category: Cell Culture Protocols: Sterile Technique Protocols

Cultured mammalian cells are used extensively in cell biology studies; it requires a number of special skills in order to be able to preserve the structure, function, behavior and biology of the cells. This unit describes the basic skills required to maintain and preserve cell cultures: aseptic technique, medium characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. - [Read Basic Techniques for Mammalian Cell Tissue Culture Protocol]

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Bird Medium Protocol: An Alternative to Vogel Medium - http://www.fgsc.net/fgn51/fgn51metz.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for bird Medium: an alternative to Vogel Medium. - [Read Bird Medium Protocol: An Alternative to Vogel Medium]

Cell Viability Assays that Measure Metabolic Capacity Protocol - http://www.promega.com/paguide/chap4.htm#title3

Category: Cell Biology and Cell Culture: Cell Viability Protocols

The CellTiter-Blue® Cell Viability Assay uses an optimized reagent containing resazurin. The homogeneous procedure involves adding the reagent directly to cells in culture at a recommended ratio of 20µl of reagent to 100µl of culture medium. - [Read Cell Viability Assays that Measure Metabolic Capacity Protocol]

Complex Yeast Media Protocols - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/media.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Complex yeast media protocols. Including: YPAD Medium or Also Called YPD plus Adenine; Synthetic Complete Drop Out (SC drop-out)Medium; Synthetic Complete drop-out Medium Mix (SC drop-out). - [Read Complex Yeast Media Protocols]

Conditioned Medium for Retinal Explant Culture Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C6.html

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Neuronal Cell Culture Media Protocols

Protocol for conditioned medium for retinal explant culture. - [Read Conditioned Medium for Retinal Explant Culture Protocol]

Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4350

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

Protocol provides methods for cryofreezing and subsequent thawing of mammalian cells. Pre-confluent cells are trypsinized, pelleted, resuspended in freezing medium, and gradually frozen. When needed, frozen cells are thawed quickly under running tap water and transferred to growth medium. - [Read Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol]

Culture of BEND Cells (Bovine Endometrial Cells) Protocol - http://www.animal.ufl.edu/hansen/protocols/bend.htm

Category: Cell Culture Protocols

Protocol for culture of BEND cells (Bovine Endometrial Cells). Includes: Culture medium, Thawing Cells, Trypsinazation/ Splitting and freezing. - [Read Culture of BEND Cells (Bovine Endometrial Cells) Protocol]

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion - http://www.animal.ufl.edu/hansen/protocols/endocult.htm

Category: Cell Culture Protocols

Protocol used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.The medium used is called Pig MEM, which is a modified minimum essential medium supplemented with non-essential amino acids, vitamins, insulin and additional glucose. - [Read Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion]

Culturing of Insect Cells and Production of Baculovirus Expressed Recombinant Proteins in 2.8 Liter - http://www.hyclone.com/pdf/procedure_insect_fernbach.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

The use of Ferbach flasks is an ideal method to amplify the cells and virus inoculum for the bioreactor. The method is also very practical for producing sufficient amounts of various proteins for feasibility studies using HyQ© SFX-Insect™ medium. The large surface area in this vessel enables sufficient aeration and, therefore, the cells, when they reach high density, are not oxygen depleted. - [Read Culturing of Insect Cells and Production of Baculovirus Expressed Recombinant Proteins in 2.8 Liter]

Direct Adaptation of Insect Cells to HyQ© SFX-Insect™ Medium - http://www.hyclone.com/pdf/procedure_insect_directadaptation.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Using this method, insect cells should be adapted to HyQ SFX-Insect in 4-8 passages. However, there are some cell lines and clones that are more sensitive to the physiochemical and nutritional changes, and in some cases it may take longer than 8 passages for the adaptation. - [Read Direct Adaptation of Insect Cells to HyQ© SFX-Insect™ Medium]

Drosophila Cornmeal, Dextrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/dextrosefood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, dextrose and yeast medium. - [Read Drosophila Cornmeal, Dextrose and Yeast Medium Protocol]

Drosophila Cornmeal, Molasses and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, molasses and yeast medium. - [Read Drosophila Cornmeal, Molasses and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/harvardfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose and yeast medium. - [Read Drosophila Cornmeal, Sucrose and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/caltechfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose, dextrose, yeast and 2-acid medium. - [Read Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol]

Drosophila Hard Agar Medium for Egg Collection Plates Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/hardagar.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila hard agar medium for egg collection plates. - [Read Drosophila Hard Agar Medium for Egg Collection Plates Protocol]

Drosophila Medium - http://flyrnai.org/RNAi_FAQ_dros_cc.html#I.

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila medium. Includes: S2, S2C, S2*, S2R+, S3, l(2)mbn, Kc(167), & DL2 cells; ML-DmBG2 & ML-DmBG6 cells; clone8 (CL8) cells. - [Read Drosophila Medium]

Drosophila Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/bloomfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila medium. - [Read Drosophila Medium Protocol]

Drosophila Semi-defined Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/germanfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila semi-defined medium. - [Read Drosophila Semi-defined Medium Protocol]

Articles (1-4 of 4)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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