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Procedure for Transfection of Mammalian Cells Using Lipofectamine - http://www.research.umbc.edu/~jwolf/m10.htm

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Lipofectamine transient tranfection of mammalian cells in IMDM cell media. Julie B. Wolf, UMBC. - [Read Procedure for Transfection of Mammalian Cells Using Lipofectamine]

Process Protocol for Hepatocyte Harvest - http://www.biolifesolutions.com/protocols/Hepatocyte_Protocol.pdf

Category: Cell Biology and Cell Culture: Hepatocyte Cell Culture

Process Protocol for Hepatocyte Harvest ... Re-suspend as necessary in culture media (for cell culture) or in CryoStor ... - [Read Process Protocol for Hepatocyte Harvest]

Protocol for Immunofluorescence - http://www.bio.unc.edu/faculty/salmon/lab/protocolsimmunofluorescence.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Protocol for Immunofluorescence - Salmon Lab. Making Boiled Donkey (or whatever) Serum (BDS) for Blocking. Making Heat-Inactivated Serum for Blocking. Mounting Media. - [Read Protocol for Immunofluorescence]

Protocol Insect Media - http://www.sigmaaldrich.com/img/assets/11020/Shields_and_Sang_M3_Insect_Media.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Insect Cell Culture Growth Media Protocols

Insect media protocol. - [Read Protocol Insect Media]

Quality Control Considerations for Cell Culture - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_9.html#Quality%2520Control

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

Quality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of the cultures and products derived from them. The main areas of quality control that are of concern for tissue culture are: The quality of the reagents and materials; The provenance and integrity of the cell lines; The avoidance of microbial contamination. - [Read Quality Control Considerations for Cell Culture]

Removal of Yeast Contamination from Lymphoblast Cultures Protocol - http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc8.html

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identification of yeast strain. - [Read Removal of Yeast Contamination from Lymphoblast Cultures Protocol]

RPMI-1640 Media Protocol - http://www.sigmaaldrich.com/img/assets/11020/RPMI_1640_Media.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for RPM1-1640 Media. - [Read RPMI-1640 Media Protocol]

SOB Media Protocol - http://www.thelabrat.com/protocols/SOB.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for SOB media. - [Read SOB Media Protocol]

SOC Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/15.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for SOC media (1000ml). - [Read SOC Media (1000 ml) Protocol]

Solutions for Hartwell's Complete (HC) Media - http://www.fhcrc.org/science/labs/gottschling/yeast/hc.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Solutions for hartwell's complete (HC) media. Includes: 10X HC (7 Dropout Amino Acid Liquid); 10X YNB; Amino Acid Solutions. - [Read Solutions for Hartwell's Complete (HC) Media]

Sporulation Guide - http://www.genomics.princeton.edu/dunham/sporulationdissection.htm

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocols and Information on sporulation. Includes: SPO++ media; 10X amino acid stock; Sporulation; Dissection. - [Read Sporulation Guide]

Sterilization of Plant Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Media_Sterilization.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for sterilization of plant culture media. Includes: Minimum Autoclaving Time for Plant Tissue Culture Medium. - [Read Sterilization of Plant Culture Media Protocol]

Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/stocksgl.html

Category: Buffers Media, and Solutions

Stock Solutions and Media. Gerard R. Lazo - [Read Stock Solutions and Media]

Storage of Bacterial Cultures Growing in Liquid Media Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4452

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Protocol describes a method for storing bacterial cultures containing glycerol which are growing in liquid media. - [Read Storage of Bacterial Cultures Growing in Liquid Media Protocol]

Storage of Bacterial Cultures Growing on Solid Medium Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4453

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Protocol describes a method for storing bacterial cultures that are growing on solid media. It is useful for storing copies of cDNA. - [Read Storage of Bacterial Cultures Growing on Solid Medium Protocol]

Terrific Broth Media Protocol - http://www.thelabrat.com/protocols/TerrificBroth.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Broth Culture Media Protocols

Protocol for terrific broth media. - [Read Terrific Broth Media Protocol]

The Cell Growth Environment - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_6.html#Environment

Category: Cell Biology and Cell Culture: Cell Culture Growth Media

Cultured cells require a sterile environment and a supply of nutrients for growth. Basic Constituents of media. Sigma - [Read The Cell Growth Environment]

Tissue Culture Methods - http://www.research.umbc.edu/~jwolf/method5.htm#tc%20methods

Category: Cell Biology and Cell Culture: Cell Culture Techniques

Tissue Culture Methods. Julie B. Wolf, Univ. Maryland. Protocols for Counting Cells, Preservation and storage of cell lines, maintenance of cell lines, media and growth, and safety guidelines for working with cells. - [Read Tissue Culture Methods]

Vitamin Mixtures for Plant Growth Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Vitamin_Mixtures.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for vitamin mixtures for plant growth media. Includes: Preparation of Solutions from Packaged Powder; Media Preparation Using Vitamin Solutions. - [Read Vitamin Mixtures for Plant Growth Media Protocol]

Vogel's Minimal Media Protocol - http://www.fgsc.net/methods/vogels.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Vogel's 50X salts as given in the Microbial Genetics Bulletin 13:42-43, 1956. In 750 ml. distilled water, dissolve successively with stirring at room temperature. - [Read Vogel's Minimal Media Protocol]

Yeast Media Protocols - http://genomics.princeton.edu/botstein/protocols.shtml

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Protocols for yeast media including: YNB1 1.1x agar, YNB2 1.1x liquid, YNB3 agar, YNB4 liquid, YNB5 Agarose, YP1 1.1x agar pH6, YP2 1.1x agar, YP3 1.1x liquid pH6, YP4 1.1x liquid, YPD1 agar pH6, YPD2 agar, YPD3 liquid pH6, YPD4 liquid. - [Read Yeast Media Protocols]

Yeast Media, Solutions and Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast11.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast Media, Solutions and Stocks Protocols. Includes: Yeast Media; AHC Medium; AHC plates; SD Medium; SD plates; Sorbitol-Agar Medium; Sorbitol-Agar Medium Top Agar; Sporulation Medium Plates; YPD Medium; YPD plates; Synthetic Media Supplements. - [Read Yeast Media, Solutions and Stocks Protocols]

YPD Media Protocol - http://www.thelabrat.com/protocols/YPDmedia.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for YPD media. - [Read YPD Media Protocol]

YPG Media Protocol - http://www.thelabrat.com/protocols/YPGmedia.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for YPG media. - [Read YPG Media Protocol]

Articles (1-9 of 9)

Lambda Phage Protocol Large DNA Preparation

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.