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Long Term Lambda Phage Storage Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda6.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

No special treatment is required to prepare a lysate for the active collection. The following procedure should be used for long-term storage of lambda clones in the archival collections. The phage are diluted in media containing 7% DMSO and frozen at -80 degrees C. - [Read Long Term Lambda Phage Storage Protocol]

Low Salt LB Media Protocol - http://www.thelabrat.com/protocols/LowSaltLB.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Broth Culture Media Protocols

Protocol for low salt LB media. - [Read Low Salt LB Media Protocol]

M17 Media Protocol - http://www.thelabrat.com/protocols/M17Media.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for M17 media. - [Read M17 Media Protocol]

M9 Minimal Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/m9minimal.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for M9 minimal media (1000 ml). - [Read M9 Minimal Media (1000 ml) Protocol]

Media and Culture of Yeast Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E5192D47CE9B0776DB7D136C34ED148A&objectid=6673ABA6C2DB2E4E7564EDFA26589256

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast. - [Read Media and Culture of Yeast Protocol]

Media and Solutions for Physarum - http://www.educationalassistance.org/Physarum/Protocols/Burland/Media.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Media and Solutions for Physarum. Tim Burland - [Read Media and Solutions for Physarum]

Media and Solutions Required for Routine ES Cell Culture Protocol - http://cmbi.bjmu.edu.cn/cmbidata/stem/culture/culture/grimwade.biochem.unimelb.edu.au/bowtell/cellbiol/sect50.htm

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for media and solutions required for routine ES cell culture protocol. - [Read Media and Solutions Required for Routine ES Cell Culture Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for media for culture of Aspergillus nidulans. Includes minimal and complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for media for culture of Aspergillus nidulans. Includes: Minimal medium; Complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Mammalian Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B6F09C1BB8FBC47FC2A7E61A8A3&objectid=66739B890A55AC13354418E67F28E693

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This protocol provides guidelines for design of serum-containing and serum-free media, selective and specialty media, and media for growth under special conditions such as soft-agar growth. - [Read Media for Culture of Mammalian Cells Protocol]

Mini-Chamber for Regulating Gaseous Environment During Culture - http://www.animal.ufl.edu/hansen/protocols/minigaschamber.htm

Category: Developmental Biology: Embryology Protocols

A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device. - [Read Mini-Chamber for Regulating Gaseous Environment During Culture]

Mounting Samples in DPX Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4460

Category: Cell Culture Protocols: Cell Fixing Protocols

Mounting media for immunohistology must be compatible with the detection method used. A suitable non-aqueous mounting medium is DPX. - [Read Mounting Samples in DPX Protocol]

Mounting Samples in Gelvatol or Mowiol Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4461

Category: Cell Culture Protocols: Cell Fixing Protocols

Mounting media for immunohistology must be compatible with the detection method used. Suitable aqueous media are made from Gelvatol or Mowiol. - [Read Mounting Samples in Gelvatol or Mowiol Protocol]

Mouse Embryonic Fibroblast Culture and Propagation Part II PDF - http://www.wicell.org/uploads/media/Protocols_Part_2_05.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

WiCell Reserarch Institute. Count MEF Cells, Trypsinize, Split, Freeze, Thaw, Irradiate, Reconstitute, Plate, Feeder. - [Read Mouse Embryonic Fibroblast Culture and Propagation Part II PDF]

Neurospora Methods Protocols - http://www.fgsc.net/methods/stanford.html

Category: Fungi Protocols: Neurospora Protocols

Protocols for Neurospora methods. Includes: Standard strains; Crosses; Minimal medium; Color coding of media; Agar substrate for manipulation and isolation; Stock solutions of supplements; Mating-type tests; Preservation of stocks by silica gel; Cleaning of glassware; Control of mites. - [Read Neurospora Methods Protocols]

NZCYM Media Protocol - http://www.thelabrat.com/protocols/NZCYM.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for NZCYM media. - [Read NZCYM Media Protocol]

NZM Media Protocol - http://www.thelabrat.com/protocols/nzm.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for NZM media. - [Read NZM Media Protocol]

NZYM Media Protocol` - http://www.thelabrat.com/protocols/nzym.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for NZYM media. - [Read NZYM Media Protocol`]

Orchid Plant Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Orchid_Culture_Media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for orchid plant culture media. - [Read Orchid Plant Culture Media Protocol]

Phage Media Preparation - http://www.rpgroup.caltech.edu/~paul/protocols/phage/media/

Category: Molecular Biology Protocols: Phage Protocols and Methods

Preparation of liquid medium for phage growth. Rob Phillips Group: Physical Biology of the Cell - [Read Phage Media Preparation]

Plant Growth Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Classic_Plant_Media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for plant growth media. - [Read Plant Growth Media Protocol]

Plant Pathology Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Pathology_Media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for plant pathology media. - [Read Plant Pathology Media Protocol]

Plant Tissue Culture Media Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Media_Preparation.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Plant Cell Culture Growth Media Protocols

Protocol for plant tissue culture media. Includes: Preparation from Packaged Powder; Preparation from Basal Salt Solutions. - [Read Plant Tissue Culture Media Protocol]

Preparation of an Ion-Exchange Column Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4195

Category: Chromatography Protocols: Ion Exchange Chromatography Protocols

Protocol for the preparation of ion-exchange chromatography column. Ion-exchange chromatography (IEC) can be used as a crude step in a protein purification scheme, or, with proper preparation, as a high-resolution step. If high resolution is desired, considerable care should be taken during column preparation, choice of IEC media, and column packing. - [Read Preparation of an Ion-Exchange Column Protocol]

Preparation of Plasmid DNA: Toothpick Minipreparation Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3905

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Plasmid DNA is prepared directly from bacterial colonies plucked from the surface of agar media with toothpicks. - [Read Preparation of Plasmid DNA: Toothpick Minipreparation Protocol]

Articles (1-9 of 9)

Lambda Phage Protocol Large DNA Preparation

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.