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ES cell culture and Media Recipies - http://saturn.med.nyu.edu/research/mp/ronlab/protocols/ES_cell_culture.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

ES cell culture and Media Recipies. Heather P. Harding - [Read ES cell culture and Media Recipies]

Expression of protein in minimal media - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/protexp.html

Category: Protein Protocols: Protein Expression Protocols

Expression of protein in minimal media using E. coli cells for expression. Dr. Chen, Biochem. & Mol. Biology, University College. London - [Read Expression of protein in minimal media]

Expression Protocol in M9 Minimal Media via T7 Promoter - http://structbio.vanderbilt.edu/chazin/wisdom/labpro/M9.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

How to prepare M9 minimal media and grow in M9 media. Chazin Lab , Vanderbilt Univ. - [Read Expression Protocol in M9 Minimal Media via T7 Promoter]

Expression Protocol in M9 Minimal Media via T7 Promoter - http://structbio.vanderbilt.edu/chazin/wisdom/labpro/M9.html

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

This protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts. Generally, M9 salts contain a nitrogen source in the form of NH4Cl. Since we want to add a labeled nitrogen source, our 5x salts are prepared minus NH4Cl. Standard 5 X M9 Minimal Media salts minus nitrogen source For 1L 5xM9 salts: - [Read Expression Protocol in M9 Minimal Media via T7 Promoter]

Fission Yeast Media Protocol - http://www-rcf.usc.edu/~forsburg/media.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for fission yeast media. Includes: YES (yeast extract with supplements) for rich complete media; YSO, for modified rich media; EMM (Edinburgh minimal medium) for minimal selective media; stock solutions to make up minimal media; MB, a very stringent minimal for transformation; Sporulation media; ME (malt extract); SPAS; EMMGlut. - [Read Fission Yeast Media Protocol]

Freezing Cells - http://www.musc.edu/BCMB/ceramide/protocols/0006.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing media, DMSO, DMSO Storage of cell lines protocol and methods. Suprya Jayadev - [Read Freezing Cells]

Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols - http://www.fgsc.net/fgn48/Kaminskyj.htm

Category: Fungi Protocols: Aspergillus Protocols

Compendium of protocols for using Aspergillus nidulans in genetic, molecular, and cell biological investigations, originally written for members of my research group. It also summarizes our common growth media and nutritional supplements, many of which originally appeared elsewhere but now are difficult to locate. Includes: Growth and storage of Aspergillus nidulans conidia; Nutritional supplements for our common auxotrophies; Double mutants; Mitotic mapping - assigning genes to chromosomes; etc - [Read Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols]

Guide For Identifying And Correcting Common Cell Growth Problems - http://www.corning.com/Lifesciences/technical_information/techDocs/cc_guide_identifing_correcting_common_cell_growth_problem_5_2_03_cls_cc_013w.pdf

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Guide For Identifying And Correcting Common Cell Growth Problems. Corning. Surface Treatment Process, Problems Related to Technique, Problems Related to Incubators, Problems Related to Culture Media, Problem Solving Suggestions. - [Read Guide For Identifying And Correcting Common Cell Growth Problems]

High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol - http://www.natureprotocols.com/2006/11/22/highthroughput_and_sensitive_a.php

Category: Pharmacology and Toxicology Protocols: Drug Discovery

High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents. Method is highly sensitive, provides quantifiable data and offers high-throughput screening capability. Starting with the treatment of cells with different doses of damaging agents, pre-prepared growing media containing 96-well plates are inoculated and cell population is automatically monitored every 10 min for 48 hours. - [Read High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol]

Hints and Precautions for the Care, Feeding and Breeding of Neurospora - http://www.fgsc.net/methods/perkins.html

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

How to Choose and Prepare Media - http://www.fgsc.net/neurosporaprotocols/How%20to%20choose%20and%20prepare%20media.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to choose and prepare media. - [Read How to Choose and Prepare Media]

How to Color-Code Media - http://www.fgsc.net/neurosporaprotocols/How%20to%20color%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information on how to color-code media. - [Read How to Color-Code Media]

Insect Cell Culture and Baculovirus Infections Protocol - http://omrf.ouhsc.edu/~frank/BACULPRO.html

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Protocol for insect cell culture and baculovirus infections. Includes: Cells, Media, Growth Characteristics, Freezing and Thawing Cells, and Glassware. - [Read Insect Cell Culture and Baculovirus Infections Protocol]

Insect Media Formulation Protocol - http://www.sigmaaldrich.com/img/assets/11020/Schneiders_Insect_Media.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Insect Cell Culture Growth Media Protocols

Protocol for insect media. - [Read Insect Media Formulation Protocol]

Insect Media Protocol - http://www.sigmaaldrich.com/img/assets/11020/Graces_Insect_Media.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Insect Cell Culture Growth Media Protocols

Protocol fo insect media. - [Read Insect Media Protocol]

Iscove’s Modified Dulbecco’s Media (IMDM) Protocol - http://www.sigmaaldrich.com/img/assets/11020/IMDM.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for Iscove’s modified dulbecco’s media (IMDM). - [Read Iscove’s Modified Dulbecco’s Media (IMDM) Protocol]

Isolation of Arabidopsis cDNAs by Complementation in Yeast - http://www.arabidopsis.org/cshl-course/12-yeast.html

Category: Plant Biology Protocols: Arabidopsis Nucleic Acid Protocols

Includes: Isolation of Arabidopsis LEU2 cDNAs by complementation of the yeast leu2 mutation; Recovery of plasmid DNA from yeast cells; Analysis of Arabidopsis cDNAs that complement a yeast leu2 mutation; Preparation of library DNA from the Lacroute cDNA library; Preparation of Yeast Media. - [Read Isolation of Arabidopsis cDNAs by Complementation in Yeast]

Lactose RNAi Plates: Minimal Lactose Media Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Lactose_RNAi_Plates.htm

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for lactose RNAi plates: minimal lactose media. - [Read Lactose RNAi Plates: Minimal Lactose Media Protocol]

Lambda DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D3.html

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Protocol describes a plate method that gives very good yield for cloning. Includes: T-TYN Media + Mg+2; T-TYN Plates; T-TYN Top Agarose. - [Read Lambda DNA Preparation Protocol]

Lambda gt22a Screening Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/L1.html

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Protocol for lambda gt22a screening. Includes: T-TYN Media + Mg+2; T-TYN Plates; T-TYN Top Agarose; Phage Dilution Buffer (PDB); 10X Kinase Buffer (-DTT); Calf Thymus DNA. - [Read Lambda gt22a Screening Protocol]

Lambda Methods Media and Solutions - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda9.html

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Information on lambda methods media and solutions. - [Read Lambda Methods Media and Solutions]

Lambda Methods Media and Solutions Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda9.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Protocol for lambda methods media and solutions. - [Read Lambda Methods Media and Solutions Protocol]

Lambda Phage DNA Quickprep - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Methods/DNAIsolation/phageQuickprep.html

Category: Molecular Biology Protocols: Phage Protocols and Methods

Lambda Phage DNA Quickprep with recipes for PSB, STE, l-dil and MgCa buffers and media. Kay Schneitz - [Read Lambda Phage DNA Quickprep]

LB broth and agar - http://hulab.tamu.edu/Protocols/Media/lb.html

Category: Buffers Media, and Solutions: Bacterial Solutions

LB broth and agar. Hu Lab. - [Read LB broth and agar]

LB Media Protocol - http://www.thelabrat.com/protocols/11.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Broth Culture Media Protocols

Protocol for LB media. - [Read LB Media Protocol]

Articles (1-9 of 9)

Lambda Phage Protocol Large DNA Preparation

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.