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2X YT Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/2xyt.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols

Protocol for 2X YT Media Recipe (1000 ml). - [Read 2X YT Media (1000 ml) Protocol]

AA & Metabolite Quantitation of Media Post-AA Labeling Protocol - http://www.duke.edu/web/ceramide/protocols/0025.html

Category: Lipid Protocols: Arachidonic Acid Protocols

Protocol for AA and metabolite quantitation of media post- AA labeling. - [Read AA & Metabolite Quantitation of Media Post-AA Labeling Protocol]

Adapting Cells to a Serum-Free Environment Protocol - https://fscimage.fishersci.com/webimages_FSC/downloads/HyClone_Protocol_8.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

There are many ways to adapt cell lines to serum-free media. Five methods are presented that are designed for adapting hybridomas to a protein-free medium. These protocols may require some modifications for your particular cell line and conditions. - [Read Adapting Cells to a Serum-Free Environment Protocol]

Antibiotic Concentration in Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/antibiot.html

Category: Buffers Media, and Solutions: Bacterial Solutions

Antibiotic Concentration in Media. (Gerard R. Lazo) - [Read Antibiotic Concentration in Media]

Arabidopsis Tissue Culture Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/tissue.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Arabidopsis Tissue Culture Stock Solutions and Media. Lazo Lab. MS MEDIUM FOR ARABIDOPSIS. B5 MEDIUM FOR ARABIDOPSIS. 10X Micronutrients. - [Read Arabidopsis Tissue Culture Stock Solutions and Media]

Bacterial Cell Maintenance Protocol - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.G

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

4 strains of E. coli are used in these studies: JM101 for M13 infection and isolation, XL1BMRF'for M13 or pUC-based DNA transformation, and ED8767 for cosmid DNA transformation. To maintain their respective F' episomes necessary for M13 viral infection, JM101 is streaked onto a M9 minimal media plate and XL1BMRF' is streaked onto an LB plate containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated at 37degC overnight. For each strain, 3 ml. of appropriate liquid.. - [Read Bacterial Cell Maintenance Protocol]

Bacterial Media Solutions and Stocks - http://humgen.wustl.edu/hdk_lab_manual/plasmid/plsmid11.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

agar, Ampicillin Stock, Alkaline Lysis Solution, SOB, SOC media, and X-gal stock solutions. Donis Keller. - [Read Bacterial Media Solutions and Stocks]

Balanced Salts for Growth Media Protocol - http://www.sigmaaldrich.com/img/assets/11020/HBS.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for balanced salts used for growth media. - [Read Balanced Salts for Growth Media Protocol]

Can you autoclave liquid Media? FAQ - http://invitrogen.custhelp.com/cgi-bin/invitrogen.cfg/php/enduser/std_adp.php?p_sid=HczfJTng&p_lva=1&p_faqid=120

Category: Cell Biology and Cell Culture: Cell Culture Growth Media

Most media cannot be autoclaved. With description. Invitrogen - [Read Can you autoclave liquid Media? FAQ]

Classic Plant Media - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Plant_Biotechnology/Tissue_Culture_Protocols/Classic_Plant_Media.html

Category: Plant Biology Protocols: Plant Tissue Culture

Includes: Chu (N6) basal salt mixture DKW/Juglans basal salt mixture Gamborg's B-5 basal salt mixture Gamborg's B-5 basal salt mixture with minimal organics Hoagland's No. 2 basal salt mixture McCown's woody plant basal salt mixture Murashige and Skoog basal salt mixture (MS) Quoirin and Lepoivre basal salt mixture Schenk and Hildebrandt basal salt mixture White's basal salt mixture - [Read Classic Plant Media]

Complex Yeast Media Protocols - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/media.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Complex yeast media protocols. Including: YPAD Medium or Also Called YPD plus Adenine; Synthetic Complete Drop Out (SC drop-out)Medium; Synthetic Complete drop-out Medium Mix (SC drop-out). - [Read Complex Yeast Media Protocols]

Culture Protocols and Information for T brucei - http://tryps.rockefeller.edu/crosslab_protocol_culture.html

Category: Bacterial Protocols

Culture protocols and information for T brucei. Detailed guide to the practice and history ofTrypanosoma brucei cultivation. This covers the culture of bloodstream and procyclic forms in liquid media and on agarose plates. - [Read Culture Protocols and Information for T brucei]

Derivation of Mouse Embryonic Fibroblasts (MEF) Part I PDF - http://www.wicell.org/uploads/media/Protocols_Part_1_05.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: MEF Protocols Mouse Embryonic Fibroblasts

WiCell Research Institute. Recipes, Frequently Asked Questions, Mouse Embryo Extraction and Tissue, Harvesting Mouse Embryonic Fibroblasts (MEF)from mice. - [Read Derivation of Mouse Embryonic Fibroblasts (MEF) Part I PDF]

DNA Transfection Mediated by Lipofection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3870

Category: Transfection Protocols

Protocol should be viewed as a starting point for systematic optimization of transfection mediated by lipofecting agents. Once a positive signal has been obtained from a transfected plasmid carrying a standard reporter gene, optimal conditions for transfection can be established by systematic variation of parameters such as the initial cell density, the amount and purity of DNA, the media and serum, and the time of exposure of the cells to the cationic-lipid-DNA complex. - [Read DNA Transfection Mediated by Lipofection Protocol]

Drosophila Cornmeal, Dextrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/dextrosefood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, dextrose and yeast medium. - [Read Drosophila Cornmeal, Dextrose and Yeast Medium Protocol]

Drosophila Cornmeal, Molasses and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, molasses and yeast medium. - [Read Drosophila Cornmeal, Molasses and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/harvardfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose and yeast medium. - [Read Drosophila Cornmeal, Sucrose and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/caltechfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose, dextrose, yeast and 2-acid medium. - [Read Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol]

Drosophila Fly Food Prep Method Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/method_press_n_seal.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila fly food prep method. - [Read Drosophila Fly Food Prep Method Protocol]

Drosophila Hard Agar Medium for Egg Collection Plates Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/hardagar.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila hard agar medium for egg collection plates. - [Read Drosophila Hard Agar Medium for Egg Collection Plates Protocol]

Drosophila Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/bloomfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila medium. - [Read Drosophila Medium Protocol]

Drosophila Semi-defined Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/germanfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila semi-defined medium. - [Read Drosophila Semi-defined Medium Protocol]

Drosophila Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/neurofood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila sucrose and yeast medium. - [Read Drosophila Sucrose and Yeast Medium Protocol]

Eagle’s Growth Media (DME) Protocol - http://www.sigmaaldrich.com/img/assets/11020/DME.pdf

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

Protocol for eagle growth media (DME). - [Read Eagle’s Growth Media (DME) Protocol]

Embryonic Stem Cell growth media Requirements - Taconic Transgenics - http://www.taconic.com/emerging/ESCells/Growth_Media_solutions.htm

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Reference: Michael P. Matise, Wotjek Auerbach and Alexandra L. Joyner (2000). Gene targeting: a practical approach. Protocol excerpted from Chapter 3, Production of targeted embryonic stem cell clones. Alexandra L. Joyner (ed.), 2nd edition, Oxford Unive - [Read Embryonic Stem Cell growth media Requirements - Taconic Transgenics]

Articles (1-9 of 9)

Lambda Phage Protocol Large DNA Preparation

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.