DNA isolation from various fungal species including: Cochliobolus, Aternaria, and Fusarium. Key steps: (1) the use of young lyophilized mycelial mats - yield less contaminating carbohydrates; (2) proteinase K in the extraction buffer to destroy DNases (f - [Read Fungal DNA Isolation Method]
Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]