materials Protocols

Search results for: materials

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Protocol for Motility using VE-DIC Microscopy - http://www.proweb.org/kinesin/Methods/VE-DIC.html

Protocol for motility using VE-DIC microscopy. Materials required: Anti-GST (glutathione S-transferase) antibody, PB buffer =10 mM NaPO4 pH 7.2, EGTA, MgCl2, Clarified motor lysate, MTs or Axoneme-MTs,MgÂ·ATP, VALAP (1 Vasoline: 1 Lanolin: 1 Paraffin, heated gently until melted) and Maxell XR-S120 Black Magnetite Super-VHS tapes or comparable. - [Read Protocol for Motility using VE-DIC Microscopy]

Protocol for Motor Polarity Using Fluorescent Microtubules - http://www.proweb.org/kinesin/Methods/Fluorescent_MT.html

Category: Cell Biology and Cell Culture

Protocol for motor polarity using fluorescent microtubules using the materials: Rhodamine-tubulin (Molecular Probes),NEM- tubulin, PC-tubulin, MgÂ·GTP, MgCl2 and Glycerol. - [Read Protocol for Motor Polarity Using Fluorescent Microtubules]

Protocol for Needle Assay for Chemotaxis - http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm

Category: Cell Biology and Cell Culture

Protocol for needle assay for chemotaxis. Materials required: Zeiss inverted microscope, eppendolf microinjector, eppendolf micromanipulator, Eppendolf femtotips, 100mM cAMP in PM,DB buffer, PM Buffer. - [Read Protocol for Needle Assay for Chemotaxis]

Protocol for Standard Fluorescence Motility Assay - http://www.proweb.org/kinesin/Methods/motility.html

Category: Cell Biology and Cell Culture

Protocol for Standard Fluorescence Motility Assay. Materials requires: Microtubules, Flow Cell, Standard Solutions: BRB80, BRB80T, BRB80CS0.5, BRB80CA, BRB80CT - [Read Protocol for Standard Fluorescence Motility Assay]

Quality Control Considerations for Cell Culture - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Culture/Key_Resources/ECACC_Handbook/Cell_Culture_Techniques_9.html#Quality%2520Control

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

Quality is important in all aspects of tissue culture since the quality of materials used i.e. media and other reagents) will affect the quality of the cultures and products derived from them. The main areas of quality control that are of concern for tissue culture are: The quality of the reagents and materials; The provenance and integrity of the cell lines; The avoidance of microbial contamination. - [Read Quality Control Considerations for Cell Culture]

Radiation Safety Manual - http://www.cdc.gov/OD/ohs/manual/radman.htm

Category: Radioactivity: Radioaction Safety

Administration of the Radiation Safety Program, , Physical Security of Radioactive Materials, "As Low as Reasonably Achievable" (ALARA) Philosophy, Authorized Users, Radiation Workers , Permitted Workers, Training, Procurement, Inventory and Records, Personnel Monitoring, Radiation Exposure During Pregnancy, Laboratory Supplies and Equipment, Bioassay, Labeling, Surveys, Radioactive Waste Disposal. U.S. Department of Health and Human Services, CDC, Atlanta, GA. - [Read Radiation Safety Manual]

Red Blood Cell Test System Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/37.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

An in vitro red blood cell assay is presented which allows the estimation of the irritation potential of tensides and tenside containing materials such as shampoos, shower gels, cleaning products, etc. The estimation is based on the fact that surfactants interact strongly with cellular membranes and proteins. Both effects are measured photometrically by use of the inherent native dye, oxyhemoglobin. - [Read Red Blood Cell Test System Protocol]

Thaw Procedure for RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000160.pdf?pid=PP00000160

Category: Cell Culture Protocols: Cell Lines Protocols

Protocol for the thaw procedure for RAW 264.7 cells. Includes: Thaw procedure and reagents and materials. - [Read Thaw Procedure for RAW 264.7 Cells]

Use of the Bradford Protein Assay in a Microtiter Plate Format - http://www.animal.ufl.edu/hansen/protocols/minibradford.htm

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

Use of the Bradford Protein Assay in a Microtiter Plate Format Protocol. Includes a short introduction to the Bradford assy, materials and procedure. - [Read Use of the Bradford Protein Assay in a Microtiter Plate Format]

Articles (1-10 of 13)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

[1-10] [11-13] [more...]

Items 26-34 out of 34 displayed.

First Previous 1 [2] Last

Total records: 34