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Advanced Methodologies in Flow Cytometry - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/index1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Advanced Methodologies in Flow Cytometry. Andrea Cossarizza et al.. Materials from a workshop held March, 1997 in Modena, Italy. - [Read Advanced Methodologies in Flow Cytometry]

Antibiotics - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Materials/antibiotics.html

Category: Microbiology: Antibiotics Concentration in Media LB

Antibiotic stock solutions preparation, Storage conditions for antibiotics, Working Concentrations for high copy plasmids. Kay Schneitz Univ Zurich. - [Read Antibiotics]

Assay of Cyclic AMP in Lysates of Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000015.pdf?pid=PP00000015

Category: Signalling Signal Transduction Protocols

Protocol allows you to measure the content of cyclic adenosine 3',5'-monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) in an enzyme-linked immunoassay. This protocol utilizes acetylation of cAMP to improve sensitivity and reduce interference. Protocol includes information on: how to determine cAMP, calculations and reagents and materials. - [Read Assay of Cyclic AMP in Lysates of Cells]

Assay of Cytokines in Tissue Culture Supernatants - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000223.pdf?pid=PP00000223

Category: Signalling Signal Transduction Protocols

Assay of cytokines in tissue culture supernatants describes a liquid suspension array for quantification of cytokines in tissue culture supernatants or serum. With this assay, it is possible to profile the level of multiple cytokines in a single well. The principle of this cytokine assay is similar to a capture sandwich immunoassay. Includes: Preparation for the Assay, Cytokine Assay, Reagents and Materials. - [Read Assay of Cytokines in Tissue Culture Supernatants]

Biuret Protein Assay - http://homepages.gac.edu/~cellab/appds/appd-g.html#exG-3

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Biuret Protein Assay. Includes Materials, methods and procedure. - [Read Biuret Protein Assay]

Clearing Arabidopsis Non-Ovule Materials With the HCG Solution Protocol - http://www.hybtech.org/Liu/HCG_clearing.htm

Category: Plant Biology Protocols

Protocol can be used for clearing intact non-ovule materials of arabidopsis, which can then be observed under Normarski optics. This is an efficient way to analyse root, seedling even flower development without sectioning. This protocol could also be used for clearing GUS stained material, after chlorophyll is removed by 70% ethanol. - [Read Clearing Arabidopsis Non-Ovule Materials With the HCG Solution Protocol]

Clearing Arabidopsis Ovule Materials with the Hoyer's Solution Protocol - http://www.hybtech.org/Liu/Hoyer_clearing.htm

Category: Plant Biology Protocols

Protocol can be used for clearing intact seeds of arabidopsis, which can then be observed under Normarski optics. This is an efficient way to analyse embryo and endosperm development without sectioning. - [Read Clearing Arabidopsis Ovule Materials with the Hoyer's Solution Protocol]

Cytogenetic Protocol Blood Preps - http://www.jax.org/cyto/blood_preps.html

Category: Cytogenetics Protocols

Cytogenetic protocol for blood preps. Includes: Preparation of materials; Culture Procedure - Use aseptic technique throughout procedure until harvest; Harvest; Slide making; Collecting blood by tail vein method; PHA Dose Response. - [Read Cytogenetic Protocol Blood Preps]

Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4123

Category: PCR Protocols

Protocol is the first in a set of three describing fluorescent mRNA differential display (FDD or FDDRT-PCR). The method begins with the harvesting of total RNA from the tissue-cultured cells of interest. For other starting materials, such as blood samples, please see Extraction and Purification of RNA from Blood Samples for Fluorescent mRNA Differential Display. - [Read Extraction and Purification of RNA from Tissue-Cultured Cells for Fluorescent mRNA Differential]

How to Deposit Materials in the Fungal Genetics Stock Center (FGSC) - http://www.fgsc.net/neurosporaprotocols/How%20to%20deposit%20materials%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to deposit materials in the Fungal Genetics Stock Center (FGSC). - [Read How to Deposit Materials in the Fungal Genetics Stock Center (FGSC)]

Immunofluorescence staining - http://www.graingerlab.org/Research/Protocols/MethodQIFstaining.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence staining. Includes Background, Procedure, Key points and materials / methods. Grainger Lab. - [Read Immunofluorescence staining]

Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol - http://www.natureprotocols.com/2007/01/25/isolation_of_arabidopsis_nucle.php

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for the isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays. Plant materials are ground in hexylene glycol-based buffers and highly enriched nuclear fractions are obtained using Percoll density gradients. Standard and small-scale protocols are presented, along with a tested method for nuclear run-on assays. The entire process may be completed within 3 days. - [Read Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol]

Laboratory Safety Manual - http://www.mcgill.ca/ehs/laboratory/labsafety/

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Manual. McGill EHS. Workplace Hazardous Materials Information System (WHMIS), Control of Chemical Hazards, Handling and Storage of Laboratory Chemicals, Fire Safety in the Laboratory, Hazardous Waste Disposal. - [Read Laboratory Safety Manual]

Live-Cell Imaging of GFP in Plants - http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.ip31

Category: Microscopy Protocols: Live-Cell Imaging Protocols

GFP serves as a molecular marker that can be imaged dynamically in living cells, both in its native form & as a fusion to other proteins. For GFP imaging, plants present the challenge of autofluorescence from chlorophyll, lignified cell walls, vacuolar contents, and other cell materials, all of which can obscure the GFP signal. Maximizing the signal-to-noise ratio is a major concern, and careful consideration should be given to the choice of tissue imaged, GFP expression level, etc. - [Read Live-Cell Imaging of GFP in Plants]

Lowry â€“ Protein Determination - http://wolfson.huji.ac.il/purification/Protocols/loury.html

Category: Protein Protocols: Lowry Protein Assay

Lowry â€“ Protein Determination. Quantitation of total protein using the lowry protein assay. Dr. Mario Lebendiker. The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem. Method materials and notes for the Lowry assay. - [Read Lowry â€“ Protein Determination]

Lowry Protein Assay Protocol - http://ylwang.umassmed.edu/protocol/pa/lowry.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay Protocol. Yu-li Wang Lab, Univ. Massachusetts Medical School. Detailed protocol including materials. - [Read Lowry Protein Assay Protocol]

MS Plant Tissue Culture Medium Protocol - http://www.unizh.ch/botinst/Cyto_Website/schneitzLab/Materials/MSmedium.html

Category: Plant Biology Protocols: Plant Tissue Culture

A recipe for MS plant medium. Details. Details of this protocol, MS Plant Tissue Culture Medium MS Plant Tissue Culture Medium Protocol - [Read MS Plant Tissue Culture Medium Protocol]

Neuroscience Histology Methods - http://psych.colorado.edu/~dbarth/PDFs/4052/4052%20Manual%20Chapters/Histology%20I.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Materials for cresyl violet staining protocol (see methods section) ... Histology also refers to the techniques used to prepare tissue for microscopic study ... Neuroscience Histology Methods - [Read Neuroscience Histology Methods]

Optimized Protocol for Mounting Tissue Sections PDF - http://arctur.com/research_portal/images/Application%20Notes/pen_membrane_protocol_9.pdf

Category: Histology Protocols: Tissue Sample Sectioning

Generally Required Materials and Equipment for Tissue Sectioning. Sectioning and Slide Preparation Procedure onto Metal-Framed PEN Membrane Slides. Arcturus. - [Read Optimized Protocol for Mounting Tissue Sections PDF]

Preparation of B-Lymphocyte Lysates for Cyclic AMP Determination - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000012.pdf?pid=PP00000012

Category: Signalling Signal Transduction Protocols

This protocol provides a sufficient sample for several determinations of cAMP using the acetylation protocol. The method chosen for measuring the content of cyclic adenosine 3',5'-monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) is an enzyme linked immunoassay system. Protocol includes information on: Treatment of Cells and Preparation of Extracts; Reagents and Materials. - [Read Preparation of B-Lymphocyte Lysates for Cyclic AMP Determination]

Preparation of B-Lymphocyte Lysates for Cyclic AMP Determination for Dual Ligand Screen - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000139.pdf?pid=PP00000139

Category: Signalling Signal Transduction Protocols

Protocol provides a method of achieving a sufficient sample for several determinations of cAMP using Amershamâ€™s acetylation protocol. Protocol includes information on: Treatment of Cells and Preparation of Extracts; Reagents and Materials. - [Read Preparation of B-Lymphocyte Lysates for Cyclic AMP Determination for Dual Ligand Screen]

Preparation of Myocyte Lysates for Cyclic AMP Determination - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000131.pdf?pid=PP00000131

Category: Signalling Signal Transduction Protocols

Protocol provides a method for acheiving a sufficient sample for several determinations of cAMP. The protocol described for measuring the content of cyclic adenosine 3',5'- monophosphate (cyclic AMP or cAMP) in cardiac myocytes is an enzyme-linked immunoassay system. Protocol includes information on: Treatment of Cells and Preparation of Extracts; Use of Environmental Chamber; Reagents and Materials. - [Read Preparation of Myocyte Lysates for Cyclic AMP Determination]

Preparation of RAW 264.7 Lysates for Cyclic AMP Determination - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000175.pdf?pid=PP00000175

Category: Signalling Signal Transduction Protocols

The protocol provides a method to achieve a sample sufficient for one determination of cAMP using the acetylation protocol. The protocol describes the method used for measuring the content of cyclic adenosine 3',5'- monophosphate (cyclic AMP or cAMP) in RAW 264.7 cells using an enzyme-linked immunoassay system. Information included in the protocol: Treatment of Cells and Preparation of Extracts; Reagents and Materials. - [Read Preparation of RAW 264.7 Lysates for Cyclic AMP Determination]

Protein Precipitation Microplate - http://www.htslabs.com/HTML/Wellplates/Applications/protein_precipitation_microplate.htm

Category: Protein Protocols: Protein Precipitation Procols

Laboratory sample cleanup is a necessary part for analytical preparation analysis. The removal of Contaminants such as proteins, cell debris and other materials is an important step. Typically this has been done by using Acetonitrile and then Centrifugation to pellet the debris leaving the clean supernant. After this process supernatant can be used for further analysis by HPLC, GC, MS and other analysis tandem methods. HTS Labs. - [Read Protein Precipitation Microplate]

Protocol for Microtubule Binding Assays - http://www.proweb.org/kinesin/Methods/MT-binding_assay.html

Category: Cell Biology and Cell Culture

Microtubule binding assay protocol using the materials: Siliconized ultracentrifuge microfuge tubes, GTP-depleted microtubules, 6X SDS loading dye, 1X SDS loading dye, Coomassie Brilliant Blue R 250 (0.8% in 50% methanol + 10% acetic acid)and Destaining solution (13% methanol + 3% acetic acid). - [Read Protocol for Microtubule Binding Assays]

Articles (1-10 of 13)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Absorption Spectroscopy and Quantification of Filamentous Phage Protocol

Unlike spherical phage, such as T4 and λ, which have roughly equal weight ratios of protein to DNA, filamentous phage have about six times more protein than DNA; the protein therefore contributes substantially to the absorption spectrum.

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