mass Protocols

Category: Cell Biology and Cell Culture

Cell Mass by Measurement of Turbidity. Cell Biology Laboratory Manual. Heidcamp - [Read Cell Mass by Measurement of Turbidity]

Category: Bacterial Cell Culture Protocols

Protocol for measurement of cell mass by turbidity. - [Read Cell Mass by Measurement of Turbidity Protocol]

Category: Molecular Biology Protocols: Carbohydrate Protocols

Characterization of Protein Glycosylation Using Chip-Based Nanoelectrospray with Precursor Ion Scanning Quadrupole Linear Ion Trap Mass Spectrometry. Sheng Zhang1 and Brian L. Williamson. Journal of Biomolecular Techniques, 16:209-219 2005. - [Read Characterization of Protein Glycosylation Using Chip-Based Nanoelectrospray with Precursor Ion Scann]

Category: Proteomic Protocols

Coomasie and SYPRO Destaining Method Protocol. MSCLS Center for Mass Spectrometry and Proteomics - [Read Coomasie and SYPRO Destaining Method]

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for determination of neopterin and biopterin by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in rat and human plasma, cell extracts and tissue homogenates. - [Read Determination of Neopterin and Biopterin by Liquid Chromatography Protocol]

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for disaggregating embryos and the ICM of blastocysts into individual cells. - [Read Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass of Blastocysts into Individual Cells]

Category: Microscopy Protocols: Electron Microscopy Protocols

The scanning transmission electron microscope precision and reproducibility of mass measurements are comparable with those of the analytical ultracentrifuge, the possibility of determining the mass not only of entire supramolecular assemblies but also of their distinct components has opened exciting new avenues which have occasionally been entered but are not yet fully explored. Includes: Principle and application (The GroEL:GroES complex). - [Read Imaging and Measuring Biomolecules & Their Assemblies by Scanning Transmission Electron Microscopy]

Category: Developmental Biology: Embryology Protocols

This protocol describes a method for isolating the ICM of blastocysts by selectively killing the outer trophectoderm (TE). - [Read Immunosurgery: Isolating the Inner Cell Mass (ICM) of Blastocysts Protocol]

Category: Mouse Protocols: Mouse Surgical Procedures Protocols

Protocol describes a method for isolating the ICM of blastocysts by selectively killing the outer trophectoderm (TE). - [Read Immunosurgery: Isolating the Inner Cell Mass (ICM) of Blastocysts Protocol]

Category: Proteomic Protocols: 2-D Gel Electrophoresis

In gel trypsin digestion for Mass spectrometry. (According to EMBL Protein and Peptide Group, 1997) - [Read In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis]

Category: Proteomic Protocols

In-Gel Digestion Protocol. Excision of protein bands (spots) from polyacrylamide gels Reduction and alkylation . Reduction and alkylation . MALDI analysis of the supernatant after in-gel digestion. Extraction of Peptides.Matthias Wilm EMBL Bioanalytical R - [Read In-gel digestion of proteins to be analyzed by mass spectrometry]

Category: Mass Spectrometry Protocols

Fast Evaporation Method for MALDI sample preparation protocol. Inserting into MALDI mass spectrometer and acquiring a spectrum. EMBL Bioanalytical Research Group. Wilm. - [Read MALDI Sample Preparation - Fast Evaporation Method]

Category: Laboratory Model Organisms Protocols

Protocol describes the culture of freshwater hypotrichs using the alga Chlorogonium elongatum as a food source. - [Read Mass Culture of Freshwater Hypotrichs Protocol]

Category: Mass Spectrometry Protocols

Protein sequencing by mass spectrometry. Mass analysis of peptides and proteins. Identification of modifications in proteins. Good information.Univ. Virginia. Dr. Nicholas Sherman - [Read Mass spectrometry analysis and identification of proteins]

Category: Mass Spectrometry Protocols

Mass spectrometry compatible silver staining of gels protocol. EMBL Bioanalytical Research Group. Wilm. - [Read Mass spectrometry compatible silver staining of gels protocol]

Category: Proteomic Protocols

ZipTip Protocol detailed protocol. MSCLS Center for Mass Spectrometry and Proteomics - [Read Millipore ZipTip detailed protocol. MSCLS]

Category: Chromatography Protocols: Gas Chromatography Protocols

Protocol describes how to determine the monosaccharide composition of glycans, glycoproteins, or proteoglycans by hydrolyzing the sample to monosaccharides and converting them to alditols, then performing acetylation to make them volatile compounds and analysis by gas chromatography (GC) or gas chromatography coupled with mass spectrometry (GC-MS). - [Read Monosaccharide Composition Analysis: Alditol Acetates Protocol]

Category: Chromatography Protocols: Affinity Chromatography Protocols

Protein complexes can be isolated by several different approaches.  For example, a protein can be tagged with an epitope such as Flag or TAP and then overexpressed in a target cell, allowing the interacting proteins to be purified.  Similarly, epitope tags can be homologously recombined into the endogenous locus ("knocked-in"), allowing protein complexes containing the tagged proteins to be isolated at their natural expression level. - [Read Overview of Affinity Purification in Combination with Mass Spectrometry Protocol]

Category: Cytogenetics Protocols

Details a placenta specific gene manipulation by transducing blastocysts with lentiviral vectors1. After a removal of zona pellucida which functions as a physical barrier, trophoblast cells lying outermost layer of blastocyst were transduced from outside with high-titer lentiviral vectors. As most placental cells descend from trophoblast cells while fetus originated from inner cell mass, transgene expression can be observed in trophoblast cells from preimplantation stages and in placenta... - [Read Placenta Specific Gene Manipulation by Transducing Zona-Free Blastocyst using Lentiviral Vector]

Category: Mass Spectrometry Protocols

This method of sample preparation produces a uniform layer of very small crystals on the mass spectrometer's sample stage that are mechanically well adhered to the substrate. It is a variant of the dried droplet method. PROWL - [Read Polycrystalline thin films Sample Preparation Protocol MALDI]

Category: Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols

Protocol is specifically for the further enrichment of phosphopeptides from a phosphotyrosine pull-down.  This is the final step for the preparation and enrichment of phosphopeptides using immobilized metal affinity chromatography (IMAC) for the identification of the phosphopeptides by liquid chromatography tandem mass spectrometry (LC-MS/MS). - [Read Preparation and Enrichment of Phosphopeptides from Phosphotyrosine Protocol]

Category: Protein Microarray Protocols

Protein microarray technology and ultraviolet crosslinking combined with mass spectrometry for the analysis of protein–DNA interactions. Gobom. - [Read Protein microarray technology and ultraviolet crosslinking combined]

Category: Proteomic Protocols

Proteomic Protocols for Sample Submission to Mass Spec. Gel Staining, In gel trypsin digestion, Coomassie Blue staining and TCA precipitation. CFG U. Albany. - [Read Proteomic Protocols for Sample Submission to Mass Spec]

Category: Mass Spectrometry Protocols

Protocols for Mass Spectrometry Analysis Preparation. Preparing gel slices for PMF or MS/MS , mass spec data interpretation, Submitting proteins in solution, Interpretation of LC-MS/MS data. BMS MASS SPECTROMETRY AND PROTEOMICS FACILITY - [Read Protocols for Mass Spectrometry Analysis Preparation]

Category: Mass Spectrometry Protocols

Silver Staining of Gels for Mass Spectrometry. Desiderio procedure: R.R. Becklin. Univ. Virginia. - [Read Silver Staining of Gels for Mass Spectrometry]