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How to Use Multiply Marked Multicent Strains for Mapping Genes and Translocation Breakpoints - http://www.fgsc.net/neurosporaprotocols/How%20to%20use%20multiply%20marked%20multicent%20strains%20for%20linkage%20group%20assignment%20of%20genes%20and%20rearrangement%20breakpoints.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to use multiply marked multicent strains for mapping genes and translocation breakpoints. - [Read How to Use Multiply Marked Multicent Strains for Mapping Genes and Translocation Breakpoints]

Plating Cells for Microinjection Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4650

Category: Cell Culture Protocols

Protocol describes a method for plating cells for microinjection onto etched coverslips. The coverslips for microinjection must be marked so that microinjected cells can be identified at time points after injection. - [Read Plating Cells for Microinjection Protocol]

Preparation of Segmented and Polarity Marked Microtubules Protocol - http://mitchison.med.harvard.edu/protocols/polarity.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Segmented and polarity-marked microtubules are very useful for many different types of in vitro assays. Segmented microtubules are microtubules with a bright seed and dim elongated segments on both ends. Polarity marked microtubules are microtubules with a bright seed and a dim elongated segment only on one end -- the plus end. - [Read Preparation of Segmented and Polarity Marked Microtubules Protocol]

Spore Germination Protocol - http://www-rcf.usc.edu/~forsburg/spores.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for spore germination. This procedure is typically used for the isolation and preparation of spores from a diploid strain heterozygous for a marked disruption (e.g., yfg1::his3+) Inoculation of the spore population into minimal medium lacking the nutritional supplement corresponding to the disruption marker (e.g., minimal medium lacking histidine) allows only the disruption spores to germinate. - [Read Spore Germination Protocol]