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PCR PRIMER DESIGN AND REACTION OPTIMISATION: Molecular Biology Techniques Manual - http://www.mcb.uct.ac.za//pcroptim.htm

Category: PCR Protocols: PCR Optimization

PCR PRIMER DESIGN AND REACTION OPTIMISATION: Molecular Biology Techniques Manual - Rybicki - [Read PCR PRIMER DESIGN AND REACTION OPTIMISATION: Molecular Biology Techniques Manual]

Protocol for Luciferase Assay for In Vitro Detection - http://aesop.rutgers.edu/~lamlab/lucassayprotocol.html

Category: Reporter Gene Assays: Luciferase Assay Protocols

Protocol for luciferase assay for in vitro detection. Protocol includes: Before cell lysate preparation; Cell lysate preparation; Protocol for manual luminometers; Protocol for plate reading luminometer; Preparation of protein assay reagent; Protein standards. - [Read Protocol for Luciferase Assay for In Vitro Detection]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Protocols for Mass Spectrometry Analysis Preparation - http://www.st-andrews.ac.uk/~bmsmspf/protocols.htm#manual

Category: Mass Spectrometry Protocols

Protocols for Mass Spectrometry Analysis Preparation. Preparing gel slices for PMF or MS/MS , mass spec data interpretation, Submitting proteins in solution, Interpretation of LC-MS/MS data. BMS MASS SPECTROMETRY AND PROTEOMICS FACILITY - [Read Protocols for Mass Spectrometry Analysis Preparation]

Radiation Safety Manual - http://www.cdc.gov/OD/ohs/manual/radman.htm

Category: Radioactivity: Radioaction Safety

Administration of the Radiation Safety Program, , Physical Security of Radioactive Materials, "As Low as Reasonably Achievable" (ALARA) Philosophy, Authorized Users, Radiation Workers , Permitted Workers, Training, Procurement, Inventory and Records, Personnel Monitoring, Radiation Exposure During Pregnancy, Laboratory Supplies and Equipment, Bioassay, Labeling, Surveys, Radioactive Waste Disposal. U.S. Department of Health and Human Services, CDC, Atlanta, GA. - [Read Radiation Safety Manual]

Radiation Safety Manual EHS Princeton - http://web.princeton.edu/sites/ehs/radmanual/radman_sec_5.htm

Category: Radioactivity: Radioaction Safety

Radiation Safety Manual EHS Princeton. Survey Instrumentation, Performing a Meter Survey, Defining Contamination, When to Survey, Where to Survey, When to Document Surveys, How to Document Surveys, When to Report Contamination, Purchase, Repair and Calibration of Survey Meters. - [Read Radiation Safety Manual EHS Princeton]

Restriction Digestion Methods - http://hg.wustl.edu/hdk_lab_manual/rdm/rdmcontents.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Digestion Methods. Donis-Kellor lab manual. Restriction Enzyme Digests, Restriction Enzyme Buffers, Restriction Digestion of Plasmid, Cosmid, and Phage DNAs. - [Read Restriction Digestion Methods]

REVERSE TRANSCRIPTION PCR: Molecular Biology Techniques Manual - http://www.mcb.uct.ac.za//pcrrt.htm

Category: RNA Protocols: RT-PCR and cDNA synthesis

REVERSE TRANSCRIPTION PCR: Molecular Biology Techniques Manual - Rybicki - [Read REVERSE TRANSCRIPTION PCR: Molecular Biology Techniques Manual]

Slide Preparation for Manual Microdissection Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/SlidePrepManualMicrodissection.html

Category: Primary Cell Isolation and Culture Protocols

Slide Preparation for Manual Microdissection for Subsequent DNA, RNA, and Protein Analysis. Manual microdissection and subsequent molecular analysis can be carried out on slides stained using standard hematoxylin and eosin methods. However, if cell types that are (or are not) expressing a specific protein are required for a study, then more advanced slide preparation methods such as Immuno-LCM may be utilized. - [Read Slide Preparation for Manual Microdissection Protocol]

SNP Genotyping System - http://www.promega.com/tbs/tm053/tm053.pdf

Category: Genetics and Genomics: Genotyping Protocols

SNP Genotyping System. Includes: Protocol for Manual READIT® Assay in Multiwell Plates; Preparation of L/L Reagent; Preparation of Heating Block; CIAP/Exo Treatment; Preparation of Interrogation Probe Solutions; Template Denaturation; Sample Interrogation; Protocol for Manual Plate Reading Luminometers; Protocol for Injecting Plate Reading Luminometers; Protocol for Automated READIT® Assay with the Biomek® 2000 Instrument. - [Read SNP Genotyping System]

Staining Manual and Fixing Manual. - http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html

Category: Histology Protocols: Histological Fixation

95% Alcoholic Bouin's Fixative, B5 Fixative, Formalin Fixative, Zenker's Fixative. Susan L. Wall Utah. - [Read Staining Manual and Fixing Manual.]

Standard PCR Protocol - Molecular Biology Techniques Manual - http://www.mcb.uct.ac.za//pcrcond.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Standard PCR Protocol - Molecular Biology Techniques Manual - Rybicki - [Read Standard PCR Protocol - Molecular Biology Techniques Manual]

Standard PCR Protocol - Molecular Biology Techniques Manual - http://www.mcb.uct.ac.za//pcrcond.htm

Category: PCR Protocols: Standard PCR Protocol

Standard PCR Protocol - Molecular Biology Techniques Manual - Rybicki - [Read Standard PCR Protocol - Molecular Biology Techniques Manual]

Transfer of Eukaryote Suspension Cultures - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-6.html

Category: Cell Biology and Cell Culture

Transfer of Eukaryote Suspension Cultures. Cell Biology Laboratory Manual. Heidcamp. - [Read Transfer of Eukaryote Suspension Cultures]

Viability Cell Count - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-7.html

Category: Cell Biology and Cell Culture

Viability Cell Count. Cell Biology Laboratory Manual. Heidcamp - [Read Viability Cell Count]

Yeast IF without Dehydration - http://genomics.princeton.edu/botstein/protocols/non_MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF without Dehydration. Botstein Lab. Protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. - [Read Yeast IF without Dehydration]