Manual measurement and manipulation of the cell surface requires access to the cells, usually in an open chamber. Temperature-controlled chambers or stage inserts are preferred for maintaining physiological activity during the experiment. For example, heated culture dishes with coverslip glass bottoms (Bioptechs) permit high-resolution fluorescence microscopy of living cells during force application. - [Read Chambers for Examination of Live Cells under Mechanical Stress Protocol]
ES Cell Culture and Manipulation- http://web.archive.org/web/20040301061220/http://dicty.cmb.nwu.edu/Chis_lab/Lab+Manual/es_cell_culture_and_manipulation.htm
Very detailed protocol. ES Cell Culture and Manipulation. Chisholm Lab, Northwestern University Medical School. - [Read ES Cell Culture and Manipulation]
Laboratory Safety Manual. McGill EHS. Workplace Hazardous Materials Information System (WHMIS), Control of Chemical Hazards, Handling and Storage of Laboratory Chemicals, Fire Safety in the Laboratory, Hazardous Waste Disposal. - [Read Laboratory Safety Manual]
There are several manual methods that can be used to perform tissue microdissection. Techniques using hand-held tools as well as mechanical micromanipulator-based approaches have been described. However, speed and precision are the most important parameters and any method that achieves these is adequate. Investigators should also expect to invest time initially by practicing on 10 to 20 cases to begin to feel comfortable with the technique. - [Read Manual Microdissection]
Protocol for luciferase assay for in vitro detection. Protocol includes: Before cell lysate preparation; Cell lysate preparation; Protocol for manual luminometers; Protocol for plate reading luminometer; Preparation of protein assay reagent; Protein standards. - [Read Protocol for Luciferase Assay for In Vitro Detection]