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Vasectomy for Generation of Sterile Males: Access via Abdominal Wall Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4376

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a method for vasectomy in which the vas deferens is accessed through the abdominal wall. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. For an alternative protocol, see Vasectomy for Generation of Sterile Males: Access via Scrotal Sac. - [Read Vasectomy for Generation of Sterile Males: Access via Abdominal Wall Protocol]

Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4377

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a method for vasectomy in which the vas deferens is accessed through the scrotal sac. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. - [Read Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols]

X-Ray Mutagenesis of Drosophila Protocol - http://www.bioprotocol.com/protocolstools/protocol.jhtml%3Bjsessionid%3DGYZOPNVU3IQQTR3FQLMSFEWHUWBNQIV0?id=p32

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol describes the general procedure for creating mutations in the DNA of Drosophila by exposure to X-rays. Irradiation of cells with X-rays creates double strand breaks (DSBs) in DNA. Mutations introduced in the DNA of germ line cells (sperm) are propagated by mating the exposed males to virgin females. The progeny of this cross can be mated to each other so that a percentage of the subsequent offspring will have two copies of the same mutant allele. - [Read X-Ray Mutagenesis of Drosophila Protocol]

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Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.