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Microinjecting C.elegans Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=5

Category: C. elegans Protocols: C. elegans Injection Protocols

Protocol for microingecting C.elegans worms. Includes: Making the DNA solution; Setting up the scope, loading the needle, mounting, and breaking the needle; Mounting worms on an injection pad; Injecting. - [Read Microinjecting C.elegans Worms Protocol]

Picking Single Phage Plaques Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda1.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Protocol for the isolation of single bacteriophage lambda plaques to be used for making lysates. - [Read Picking Single Phage Plaques Protocol]

Protocol for Immunofluorescence - http://www.bio.unc.edu/faculty/salmon/lab/protocolsimmunofluorescence.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Protocol for Immunofluorescence - Salmon Lab. Making Boiled Donkey (or whatever) Serum (BDS) for Blocking. Making Heat-Inactivated Serum for Blocking. Mounting Media. - [Read Protocol for Immunofluorescence]

Rapid Method for Making Large Numbers of Crosses Protocol - http://www.fgsc.net/neurosporaprotocols/Rapid__crossesDE.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for a rapid method for making large numbers of crosses. - [Read Rapid Method for Making Large Numbers of Crosses Protocol]

RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_in_situ.html

Category: C. elegans Protocols

Protocol for RNA in situ hybridization of dissected gonads with digoxygenin-labeled probes. Includes: Preparation of single-stranded probes from cloned cDNA; Dissections and Fixation; Proteinase K digestion; Hybridization; Making a Mount. - [Read RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes Protocol]

SECTIONING RESIN EMBEDDED TISSUE - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Histology Protocols: Tissue Sample Sectioning

SECTIONING RESIN EMBEDDED TISSUE. MAKING LATTA-HARTMANN KNIVES and Sectioning Protocols for Histology. University of Nottingham Medical School Division of Histopathology. James Lowe. - [Read SECTIONING RESIN EMBEDDED TISSUE]

Sectioning Resin Embedded Tissue Protocol - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for sectioning resin embedded tissue. Includes: MAKING LATTA-HARTMANN KNIVES; SECTIONING. - [Read Sectioning Resin Embedded Tissue Protocol]

Setting Up Microdrop Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4353

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol describes how to set up microdrop cultures to produce embryos which can then be used for making chimeras. The microdrop culture should be set up several hours to 1 day before the experiment to permit temperature and gas equilibration. - [Read Setting Up Microdrop Cultures Protocol]

Theory: How to Make Simple Solutions and Dilutions - http://abacus.bates.edu/~ganderso/biology/resources/dilutions.html

Category: Buffers Media, and Solutions

Theory: How to Make Simple Solutions and Dilutions. Information on dilution making. - [Read Theory: How to Make Simple Solutions and Dilutions]

Transformation of Aspergillus niger Protocol - http://clone.concordia.ca/aspergillus/protocols/nigtransf.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for the transformation of Aspergillus niger. This procedure is done by first digesting the outer cell wall, forming protoplasts, and then by making holes in the membrane through which the dna can enter using calcium chloride and polyethylene glycol. Includes: Protocol for making A.niger protoplasts; Transformation; Plating. - [Read Transformation of Aspergillus niger Protocol]

Xenopus Whole Mount In Situ Hybridization Protocols - http://tropicalis.berkeley.edu/home/gene_expression/in-situ/insitu.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for Xenopus whole mount in situ hybridization. Includes: Protocols for Making Antisense RNA probe (dig labelled); Protocols for In Situ Hyb. - [Read Xenopus Whole Mount In Situ Hybridization Protocols]

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Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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