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96-Well Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/96_well_confirmation.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for 96-well confirmation Yeast PCR. Includes: Clonal purification; Generate a master plate (96-well format); Making a frozen backup stock; Confirmation PCR for one Row; ORF Specific Confirmation PCR --> "A-B" primers (upstream junction); Transfer template DNA to multiwell PCR plate; Prepare and dispense master mix for A-B PCR. - [Read 96-Well Confirmation Yeast PCR Protocol]

Agarose Gel Electroporesis of DNA Protocol - http://www.unc.edu/~fbottone/protocol/electro.html

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Protocol for agarose gel electrophoresis of DNA. Includes: Making the gel; Loading the gel; Running the gel.
- [Read Agarose Gel Electroporesis of DNA Protocol]

Buffer calculator tool - http://molbiol.ru/eng/protocol/01_07.html

Category: Buffers Media, and Solutions

Buffer calculator tool for commonly used buffer compound solutions. Making different volumes? use this! (Practical Molecular Biology) - [Read Buffer calculator tool]

Cajal Body Isolation Protocol - http://www.lamondlab.com/f7cbprotocol.htm

Category: Cell Organelle Protocols: Nucleus Protocols

Cajal Body Isolation Protocol. Protocol includes: Sonication, Removal Nucleoi, Gradient One, Gradient two, Concentration and final enrichment of cajal bodies. Also includes: Making 2.55M sucrose stock and Analysis of the enriched Cajal body fraction. - [Read Cajal Body Isolation Protocol]

Cytogenetic Protocol Blood Preps - http://www.jax.org/cyto/blood_preps.html

Category: Cytogenetics Protocols

Cytogenetic protocol for blood preps. Includes: Preparation of materials; Culture Procedure - Use aseptic technique throughout procedure until harvest; Harvest; Slide making; Collecting blood by tail vein method; PHA Dose Response. - [Read Cytogenetic Protocol Blood Preps]

Darkfield Illumination - http://microscopy.fsu.edu/primer/techniques/darkfield.html

Category: Microscopy Protocols: Optical Microscopy Protocols

The visibility of the faint star light is enormously enhanced against a dark background. This principle is applied in darkfield (also called darkground) microscopy, a simple and popular method for making unstained transparent specimens clearly visible. Such objects often have refractive indices very close in value to that of their surroundings and are difficult to image in conventional brightfield microscopy. - [Read Darkfield Illumination]

Elispot plates column - http://www.zellnet.com/elispotplatescolumn/

Category: ELISA Protocols: ELISpot Protocols

Tips for selecting the â€œrightâ€ ELISPOT filter plate and making the best protocol choices. Alan J. Weiss ZellNet - [Read Elispot plates column]

Flow cytometric determination of leukocyte surface antigens in whole blood - http://pingu.salk.edu/flow/protocols/wholeblood.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Flow cytometric determination of leukocyte surface antigens in whole blood. Quantitation of cell surface antigens in whole blood with the flow cytometer is very simple and requires:
1. Blood collection; 2. Addition of antibody; 3. Calibration of the flow cytometer; 4. Making measurements. - [Read Flow cytometric determination of leukocyte surface antigens in whole blood]

How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab - http://www.fgsc.net/neurosporaprotocols/How%20to%20avoid%20pitfalls%20and%20keep%20out%20of%20trouble.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

Information to Make Antibodies against Peptides - Pierce - http://www.piercenet.com/y/production/producebottom.html

Category: Peptide Protocols: Antibodies from Peptides Protocols

Great resource for information. on making antibodies against peptides - Pierce - [Read Information to Make Antibodies against Peptides - Pierce]

Lab Faqs: Buffers Roche - http://www.roche-applied-science.com/labfaqs/p4_1.htm

Category: Buffers Media, and Solutions

Lab Faqs: Buffer recipies. Whether you are making TAE or TBE this has got it all...Roche - [Read Lab Faqs: Buffers Roche]

Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate - http://www.oup.co.uk/pdf/pas/6-5-3.pdf

Category: DNA Microarray Protocols

Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate. Elder et al., Oxford Practical Approach Series. - [Read Making a scanning array: in situ synthesis of oligonucleotides on a solid substrate]

Making a Tetrad Dissection Needle Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4182

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Microneedles attached to micromanipulators are used in the dissection of tetrads, isolation of zygotes from populations of mating haploid cells, and manipulation of individual cells. - [Read Making a Tetrad Dissection Needle Protocol]

Making and Injecting Xenopus Embryos Protocol - http://spot.colorado.edu/~klym/Methods/embryos.htm

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for making and injecting Xenopus embryos. Includes: Injections; Egg Activation and Inversion Experiments; - [Read Making and Injecting Xenopus Embryos Protocol]

Making antibodies to synthetic peptides - http://medicine.ucsd.edu/hypertension/list_of_protocols/16_antibodies_to_peptides.htm

Category: Peptide Protocols: Antibodies from Peptides Protocols

Protocol for Antibody Creation from a Synthetic Peptide. Antibody from Peptides. Daniel O'Connor. - [Read Making antibodies to synthetic peptides]

Making Antibodies to Synthetic Peptides Protocol - http://medicine.ucsd.edu/hypertension/list_of_protocols/16_antibodies_to_peptides.htm

Category: Antibody Protocols: Antibody Production Protocols

Protocol for making antibodies to synthetic peptides. - [Read Making Antibodies to Synthetic Peptides Protocol]

Making Dauers with Dauer Pheromone Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=13

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for making Dauers with dauer pheromone. - [Read Making Dauers with Dauer Pheromone Protocol]

Making Holding Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4394

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes a method for making holding pipettes, to be used for microinjection. - [Read Making Holding Pipettes Protocol]

Making Holding Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4394

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for making holding pipettes, to be used for microinjection. - [Read Making Holding Pipettes Protocol]

Making Injection Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4395

Category: Microinjection Protocols: Microinjection Equipment

This protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection. - [Read Making Injection Pipettes Protocol]

Making Injection Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4395

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection. - [Read Making Injection Pipettes Protocol]

MAKING MICROARRAY PRINTING PLATES (IN DMSO) - http://pga.tigr.org/sop/M003_1a.pdf

Category: DNA Microarray Protocols

This protocol describes the method for making microarray printing plates in a 96 well format. (The same procedure applies to a 384 well format as well.) Hasseman. TIGR Microarray Protocols - [Read MAKING MICROARRAY PRINTING PLATES (IN DMSO)]

Making Microtubule Seeds Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mtseeds.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for making microtubule seeds. - [Read Making Microtubule Seeds Protocol]

Making Monoclonal Antibodies Protocol - http://dicty.cmb.northwestern.edu/Chis_lab/Lab%20Manual/immunological_techniques.htm

Category: Antibody Protocols: Antibody Production Protocols

Protocol for making monoclonal antibodies. - [Read Making Monoclonal Antibodies Protocol]

Making Pipettes From Hard Glass Capillaries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4354

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for making pipettes from hard glass capillaries. - [Read Making Pipettes From Hard Glass Capillaries Protocol]

Articles (1-3 of 3)

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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