macronuclei Protocols

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Isolation and Purification of Tetrahymena Nuclei Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4500

Category: Tetrahymena Protozoa Protocols

Protocol describes how to allow the isolation of nuclei from all stages of the Tetrahymena life cycle in high yield with a high degree of purity. This method gives highly purified populations of both micronuclei and macronuclei. - [Read Isolation and Purification of Tetrahymena Nuclei Protocol]

Transformation of Tetrahymena thermophila by Electroporation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4502

Category: Tetrahymena Protozoa Protocols

Protocol describes a method for transformation of the Tetrahymena using electroporation. The vector is electroporated into cells after mating, where it is incorporated into the DNA of developing macronuclei. Because T. thermophila can be propagated indefinitely without conjugation, transformation of the macronucleus provides a way to obtain stable somatic transformants. DNA vectors transformed using this protocol include those containing drug-resistant versions of Tetrahymena genes. - [Read Transformation of Tetrahymena thermophila by Electroporation Protocol]

Transformation of Tetrahymena thermophila by Electroporation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4502

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Protocol describes a method for transformation of the Tetrahymenausing electroporation. The vector is electroporated into cellsafter mating, where it is incorporated into the DNA of developingmacronuclei. Because T. thermophila can be propagated indefinitelywithout conjugation, transformation of the macronucleus providesa way to obtain stable somatic transformants. - [Read Transformation of Tetrahymena thermophila by Electroporation Protocol]