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Solutions for Hartwell's Complete (HC) Media - http://www.fhcrc.org/science/labs/gottschling/yeast/hc.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Solutions for hartwell's complete (HC) media. Includes: 10X HC (7 Dropout Amino Acid Liquid); 10X YNB; Amino Acid Solutions. - [Read Solutions for Hartwell's Complete (HC) Media]

Stable Isotope Labeling by Amino Acids in Cell Culture SILAC Protocol - http://www.natureprotocols.com/2007/01/11/a_practical_recipe_for_stable.php

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for stable isotope labeling by amino acids in cell culture (SILAC). Protocol describes how to apply SILAC and the use of nano-scale liquid chromatography coupled to electrospray ionization mass spectrometry for protein identification and quantification. This procedure can be completed in 8 days. - [Read Stable Isotope Labeling by Amino Acids in Cell Culture SILAC Protocol]

Sterilization and Filtration Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B9D0482A943EC97AC4807854493&objectid=6673A220D50910E56F81295AE515690F

Category: Cell Culture Protocols: Sterile Technique Protocols

This unit on sterility in the tissue culture environment describes methods for sterilization of liquid and dry goods used for tissue culture and filtration of liquids to prevent contamination of cultures. - [Read Sterilization and Filtration Protocol]

Storage of Bacterial Cultures Growing in Liquid Media Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4452

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Protocol describes a method for storing bacterial cultures containing glycerol which are growing in liquid media. - [Read Storage of Bacterial Cultures Growing in Liquid Media Protocol]

The Liquid Scintillation Counting Process - http://laxmi.nuc.ucla.edu:8248/M248_99/autorad/Scint/scint1.html

Category: Radioactivity: Liquid Scintillation Counting

The Liquid Scintillation Counting Process. Great Review of the Process and detection procedure of the counter. Dr. Gambhir - [Read The Liquid Scintillation Counting Process]

Yeast DNA Isolation: Midiprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4147

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast DNA isolation. Protocol describes the preparation of DNA from a 40-ml liquid culture. - [Read Yeast DNA Isolation: Midiprep Protocol]

Yeast DNA Isolation: Miniprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4148

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of DNA from a 5-ml liquid culture of yeast. - [Read Yeast DNA Isolation: Miniprep Protocol]

Yeast Media Protocols - http://genomics.princeton.edu/botstein/protocols.shtml

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Protocols for yeast media including: YNB1 1.1x agar, YNB2 1.1x liquid, YNB3 agar, YNB4 liquid, YNB5 Agarose, YP1 1.1x agar pH6, YP2 1.1x agar, YP3 1.1x liquid pH6, YP4 1.1x liquid, YPD1 agar pH6, YPD2 agar, YPD3 liquid pH6, YPD4 liquid. - [Read Yeast Media Protocols]

Articles (1-6 of 6)

Lambda Phage Protocol Large DNA Preparation

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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