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Amplification and Storage of a Cosmid Library: Amplification in Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4001

Category: DNA Protocols: Cosmid Library Protocols

Amplification of cosmid libraries may result in distorted representation of cloned genomic sequences and should be avoided wherever possible. If amplification should become necessary, it is best to expand the library by growth in liquid medium. - [Read Amplification and Storage of a Cosmid Library: Amplification in Liquid Culture Protocol]

Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography - http://www.glycotech.com/protocols/Proto6.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

High performance liquid affinity chromatography (HPLAC) is a useful procedure to investigate he interactions between carbohydrate binding protein and their ligands. Technical requirements are similar to conventional HPLC. HPLAC can screen and separate natural ligands from complex biological mixtures. WeiTong Wang~GlycoTech Corporation, Rockville, Maryland - [Read Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography]

Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography - http://www.glycotech.com/protocols/Proto6.html

Category: HPLC Protocols

Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography Protocol. Glycotech. - [Read Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography]

Assay of Cytokines in Tissue Culture Supernatants - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000223.pdf?pid=PP00000223

Category: Signalling Signal Transduction Protocols

Assay of cytokines in tissue culture supernatants describes a liquid suspension array for quantification of cytokines in tissue culture supernatants or serum. With this assay, it is possible to profile the level of multiple cytokines in a single well. The principle of this cytokine assay is similar to a capture sandwich immunoassay. Includes: Preparation for the Assay, Cytokine Assay, Reagents and Materials. - [Read Assay of Cytokines in Tissue Culture Supernatants]

Assay of Cytokines in Tissue Culture Supernatants Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000223.pdf?pid=PP00000223

Category: Immunology: Immunoassays

The Bio-Plex cytokine assay employs a liquid suspension array for quantification of cytokines in tissue culture supernatants or serum. Using this 96-well microtiter plateformatted assay, it is possible to profile the level of multiple cytokines in a single well. - [Read Assay of Cytokines in Tissue Culture Supernatants Protocol]

Bacterial Cell Maintenance Protocol - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.G

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

4 strains of E. coli are used in these studies: JM101 for M13 infection and isolation, XL1BMRF'for M13 or pUC-based DNA transformation, and ED8767 for cosmid DNA transformation. To maintain their respective F' episomes necessary for M13 viral infection, JM101 is streaked onto a M9 minimal media plate and XL1BMRF' is streaked onto an LB plate containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated at 37degC overnight. For each strain, 3 ml. of appropriate liquid.. - [Read Bacterial Cell Maintenance Protocol]

Bacterial Glycerol Stocks Protocol - http://www.unc.edu/depts/van_dyke/protocols/bacteria/bacterial_glycerol_stocks.pdf

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

To 2mls of mid-log culture or 1ml of freshly saturated culture add 1ml(or an equal volume) of glycerol solution, mix gently, then freeze rapidly in liquid nitrogen or on dry ice. Store at -20°C and - 70°C. - [Read Bacterial Glycerol Stocks Protocol]

Beta-galactosidase Reporter Gene Assay (Liquid Form) - http://www.med.unc.edu/~hdohlman/bgal.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Protocol for Beta-galactosidase reporter gene assay (liquid form). - [Read Beta-galactosidase Reporter Gene Assay (Liquid Form)]

Can you autoclave liquid Media? FAQ - http://invitrogen.custhelp.com/cgi-bin/invitrogen.cfg/php/enduser/std_adp.php?p_sid=HczfJTng&p_lva=1&p_faqid=120

Category: Cell Biology and Cell Culture: Cell Culture Growth Media

Most media cannot be autoclaved. With description. Invitrogen - [Read Can you autoclave liquid Media? FAQ]

CAT Assay (Liquid Phase) Protocol - http://snbworld.com/cat2.html

Category: Reporter Gene Assays: Cat Assays Protocols

Protocol describing a CAT assay (liquid phase). - [Read CAT Assay (Liquid Phase) Protocol]

CAT.INIST.FR - http://cat.inist.fr/?aModele=afficheN&cpsidt=16477965

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

We have established a high-throughput protocol for mouse genotyping using two robotics workstations: a liquid-handling robot to assemble PCR and a ... - [Read CAT.INIST.FR]

Cell Freezing using Liquid Nitrogen N2 - http://www.liv.ac.uk/~giles/methods/cell_freezing.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing gealthy cells (> 95% viable) the protocol obtains a culture 80-90% viable 24 hours after thawing and growing on test vial from step 9. Antisense Research Group - [Read Cell Freezing using Liquid Nitrogen N2]

Culture of Human Prostatic Carcinoma Cell Lines - http://www.celldeath.de/apometh/rpmi.html

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

Culture of Human Prostatic Carcinoma Cell Lines, Growing and splitting the cells, Preparation of frozen stocks in liquid nitrogen, How to bring frozen cells back into culture, Concentrations of antibiotics for the selection of stable transfectants. LNCa - [Read Culture of Human Prostatic Carcinoma Cell Lines]

Culture Protocols and Information for T brucei - http://tryps.rockefeller.edu/crosslab_protocol_culture.html

Category: Bacterial Protocols

Culture protocols and information for T brucei. Detailed guide to the practice and history ofTrypanosoma brucei cultivation. This covers the culture of bloodstream and procyclic forms in liquid media and on agarose plates. - [Read Culture Protocols and Information for T brucei]

Defrosting Eukaryotic Cells Frozen In Liquid N2 Protocol - http://www2.umdnj.edu/~geller/lab/thawing.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

Protocol for the defrosting of eukaryotic cells frozen in liquid N2. - [Read Defrosting Eukaryotic Cells Frozen In Liquid N2 Protocol]

Detection and Measurement of Radioactivity - http://www.ruf.rice.edu/%7Ebioslabs/methods/radioisotopes/rad1.html

Category: Radioactivity

Radioactive Decay, Methods of detection, Liquid scintillation counting, Background and Quenching, The scintillation counter printout. David R. Caprette, Rice Univ. - [Read Detection and Measurement of Radioactivity]

Flow Cytometry Analysis Protocol - http://cbr.med.harvard.edu/labs/springer/protocols/qing_facs.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Flow Cytometry Analysis Protocol. Springer Lab - Harvard. Background on flow cytometry technique: Flow cytometry is a method which uses instrumentation scanning single cells flowing past excitation sources in a liquid medium.
- [Read Flow Cytometry Analysis Protocol]

Freezing and Thawing Eukaryotic Cells - http://omrf.ouhsc.edu/~frank/EukFreeze.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing and Thawing Eukaryotic Cells in liquid nitrogen, Freezing Cell Lysates for Western Blots, Thawing Cells from Liquid Nitrogen. Dr. Bart Frank Oklahoma. - [Read Freezing and Thawing Eukaryotic Cells]

Freezing and Thawing of Mammalian Cell Lines - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing and Thawing of Mammalian Cell Lines. For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. Kitto Lab Texas. - [Read Freezing and Thawing of Mammalian Cell Lines]

Freezing and Thawing of Mammalian Cell Lines Protocol - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. - [Read Freezing and Thawing of Mammalian Cell Lines Protocol]

Growing Bacteriophage M13 in Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3994

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Most manipulations with M13, including preparations of viral stocks and isolation of single- and double-stranded DNAs, begin with small-scale liquid cultures that are infected with an M13 plaque, picked from an agar plate. - [Read Growing Bacteriophage M13 in Liquid Culture Protocol]

Growth and Purification of 25-100 ug Lambda Clone DNA Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda13.html

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

There are essentially three parts to this protocol: 1. growth of at least 5x10e8 pfu phage to provide an inoculum growth of a larger liquid lysate that will produce about 5x10e12 pfu; 2. concentration and purification of the phage, and; 3. DNA preparation. - [Read Growth and Purification of 25-100 ug Lambda Clone DNA Protocol]

High Molecular Weight Yeast Liquid DNA Preparation Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast4.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol that isolates intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. Using this protocol one can expect a yield of 100-200 µg of DNA per prep. - [Read High Molecular Weight Yeast Liquid DNA Preparation Protocol]

High-resolution SNP mapping by denaturing HPLC - http://www.pnas.org/cgi/content/full/99/16/10575

Category: DNA Protocols: SNP Protocols

High-resolution SNP mapping by denaturing HPLC. A SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature of the SNPs. They demonstrate the use of denaturing high-performance liquid chromatography to identify mutations in the candidate genes and to fine-map chromosomal breakpoints. - [Read High-resolution SNP mapping by denaturing HPLC]

HPLC Protocol - http://tesla.ccrc.uga.edu/group_pages/protocols/downloads/HPLCProtocol.doc

Category: HPLC Protocols

HPLC Protocol. A general HPLC procedure to get you started on High Performance Liquid Chromatography. - [Read HPLC Protocol]

Articles (1-6 of 6)

Lambda Phage Protocol Large DNA Preparation

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Probe Preparation for 22 x 40 mm DNA Microarrays

Probe Preparation for 22 x 40 mm DNA Microarrays Protocol.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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