lineage Protocols

Search results for: lineage

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

BDA Mouse Lineage Tracing on Sections Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/Pdfs/BDA%20staining%20on%20section.pdf

Category: Mouse Protocols

Protocol describes the strongest way for lineage tracing on sections by using biotin dextran amine. - [Read BDA Mouse Lineage Tracing on Sections Protocol]

Biogenesis and Function of Peroxisomes and Glycosomes - http://students.washington.edu/mdminer/pabio_551/kessler1.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Peroxisome Isolation Protocols

Peroxisomes of higher eukaryotes, glycosomes of kinetoplastids, & glyoxysomes of plants are related microbody organelles that perform differing metabolic functions tailored to their cellular environments. The close evolutionary relationship of these organelles is most clearly evidenced by the conservation of proteins involved in matrix protein import and biogenesis. glycosome can be viewed as an offshoot of the peroxisomal lineage with additional metabolic functions, specifically glycolysi - [Read Biogenesis and Function of Peroxisomes and Glycosomes]

CHAPOL Lineage Analyis Protocol - http://genetics.med.harvard.edu/~cepko/protocol/CHAPOL.pdf

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Protocol for CHAPOL lineage analysis. - [Read CHAPOL Lineage Analyis Protocol]

Lineage Analysis Using Retroviral Vectors - http://genetics.med.harvard.edu/~cepko/lineage/

Category: Developmental Biology

Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues. An overview of the relevant aspects of the retroviral life cycle, and the strategies and current methods in use at their laboratory are described. - [Read Lineage Analysis Using Retroviral Vectors]

Lineage Analysis Using Retroviral Vectors Protocol - http://genetics.med.harvard.edu/~cepko/lineage/

Category: Cloning Protocols: Vector Protocols

Protocol describes the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues. - [Read Lineage Analysis Using Retroviral Vectors Protocol]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Antibodies for Lineage Depletion; Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Immunology

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol]