ligate Protocols

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Blunt-end Cloning of PCR Products Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3830

Category: PCR Protocols: PCR Cloning Protocols

Protocol for blunt-end cloning of PCR products. Incubation of a blunt-end ligation reaction in the presence of an excess amount of an appropriate restriction enzyme can dramatically increase the yield of recombinant plasmids. The role of the restriction enzyme is to cleave circular and linear concatemers at restriction sites that are re-formed when linear, blunt-ended plasmid molecules ligate to themselves. I - [Read Blunt-end Cloning of PCR Products Protocol]

Construction of a Yeast Genomic Library Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p361

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol for the construction of a Yeast genomic library. Includes: Prepare the genomic DNA; Prepare the Library Vector; Ligate the Digested Genomic DNA to the Digested Vector DNA; Prepare Library DNA from Bacteria. - [Read Construction of a Yeast Genomic Library Protocol]

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Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

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