ligase Protocols

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5'-End cDNA Amplification Using New RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4132

Category: PCR Protocols: cDNA Synthesis Protocols

New RACE, a variation of RNA ligase-mediated-RACE (RLM-RACE) (Liu and Gorovsky 1993) departs from classic RACE (see 5'-End cDNA Amplification Using Classic RACE) in that an "anchor" primer is attached to the 5'-end of the mRNA before the reverse transcription step; hence the anchor sequence becomes incorporated into the first-strand cDNA if, and only if, the reverse transcription proceeds through the entire length of the mRNA of interest. - [Read 5'-End cDNA Amplification Using New RACE Protocol]

DNA Insert Ligation into Vector DNA (with T4 DNA Ligase) - http://www.fermentas.com/techinfo/modifyingenzymes/protocols/p_dnainslig.htm

Category: Molecular Biology Protocols: DNA Ligation Protocols

Using digested vector DNA (50-400ng) and foreign DNA to be inserted. Fermentas - [Read DNA Insert Ligation into Vector DNA (with T4 DNA Ligase)]

DNA Ligation Protocol PDF - http://www.oup.co.uk/pdf/pas/9v1-7-9.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligation Method, Equipment and reagents, T4 DNA ligase. Gannon and Powell. Oxford Practical Approach Series. - [Read DNA Ligation Protocol PDF]

DNA ligation with Linearized DNA - http://www.genome.ou.edu/protocol_book/protocol_partII.html#II.D

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligations are performed by incubating DNA insert with linearized cloning vector in the presence of ligation buffer, rATP, and T4 DNA ligase. Roe. Univ. Oklahoma. - [Read DNA ligation with Linearized DNA]

Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3929

Category: Cloning Protocols

Protocol for ligating plasmid and target DNAs in low-melting-temperature agarose. Ligation in low-melting-temperature agarose is much less efficient than ligation with purified DNA in free solution and requires a large amount of DNA ligase. The method is used chiefly for rapid subcloning of segments of DNA in dephosphorylated vectors and assembling recombinant constructs. - [Read Ligating Plasmid and Target DNAs in Low-melting-temperature Agarose Protocol]

Linker Ligation (with T4 DNA Ligase) - http://www.fermentas.com/techinfo/modifyingenzymes/protocols/p_linkerligation.htm

Category: Molecular Biology Protocols: DNA Ligation Protocols

Linker Ligation (with T4 DNA Ligase) Fermentas. - [Read Linker Ligation (with T4 DNA Ligase)]

Protocol for the quick ligation with T4 DNA ligase - http://hincklab.uthscsa.edu/html/protocols/quick_ligation_protocol.shtml

Category: Molecular Biology Protocols: DNA Ligation Protocols

Quick Ligation using PEG 6000 and T4 DNA ligase. Hinck Lab. - [Read Protocol for the quick ligation with T4 DNA ligase]

Self-circularization of Linear DNA (with T4 DNA Ligase) - http://www.fermentas.com/techinfo/modifyingenzymes/protocols/p_self_cirlineardna.htm

Category: Molecular Biology Protocols: DNA Ligation Protocols

Self-circularization of Linear DNA (with T4 DNA Ligase). Fermentas - [Read Self-circularization of Linear DNA (with T4 DNA Ligase)]

Articles (1-2 of 2)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

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