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Complete Microarray Chip Protocol - http://www.rbhrfcrc.qimr.edu.au/kidney/Pages/Microarray%20protocol.html

Reagents & Equipment, Labelling, Purification, Hybridisation, Washing. KRL Kidney Research Dr.Healt - [Read Complete Microarray Chip Protocol]

Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion - http://www.animal.ufl.edu/hansen/protocols/endocult.htm

Category: Cell Culture Protocols

Protocol used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.The medium used is called Pig MEM, which is a modified minimum essential medium supplemented with non-essential amino acids, vitamins, insulin and additional glucose. - [Read Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion]

EMSA Protocol using 32-p. - http://www.well.ox.ac.uk/flint/EMSA.htm

Category: DNA Protocols: EMSA DNA-Protein Protocols

Primer design, Primer annealing, Primer labelling, Probe purification, In vitro hybridization DNA-protein Binding reaction. Jonathan Flint. - [Read EMSA Protocol using 32-p.]

Expression of F-tryptophan labelled protein - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/fluoexp.html

Category: Protein Protocols: Protein Expression Protocols

Expression of F-tryptophan labelled protein. Protocol can be adapted for Fluoro-phenylalanine or Fluro-tyrosine labelling. Transformation of competent E. coli cells for expression with plasmid. Dr. Chen, Dept. of Biochem. & Mol. Biology, University College, London. - [Read Expression of F-tryptophan labelled protein]

Labelling Drosophila pupae with BrdU Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_Pupal_Injection.pdf

Category: Drosophila Protocols: Drosophila Dissection Protocols

Protocol for labelling Drosophila pupae with BrdU. - [Read Labelling Drosophila pupae with BrdU Protocol]

Random Labelling DNA for array CGH Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/Labelling.shtml

Category: Cytogenetics Protocols: Array CGH

Random Labelling DNA for array CGH Protocol. Sanger Institute - [Read Random Labelling DNA for array CGH Protocol]

Articles (1-1 of 1)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.