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BAC-FISH Protocol - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/BAC_FISH.html

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. Includes: Labeling of BAC clones; Ethanol precipitation; Hybridization; Post-hybridisation treatment / detection. - [Read BAC-FISH Protocol]

Expression of F-tryptophan labelled protein - http://www.biochem.ucl.ac.uk/bsm/nmr/protocols/protocols/fluoexp.html

Category: Protein Protocols: Protein Expression Protocols

Expression of F-tryptophan labelled protein. Protocol can be adapted for Fluoro-phenylalanine or Fluro-tyrosine labelling. Transformation of competent E. coli cells for expression with plasmid. Dr. Chen, Dept. of Biochem. & Mol. Biology, University College, London. - [Read Expression of F-tryptophan labelled protein]

GFP-Labelled Crest Transplant Protocol - http://spot.colorado.edu/~klym/Methods/cresttransplant.htm

Category: Xenopus Protocols

Protocol for GFP-labelled crest transplant. - [Read GFP-Labelled Crest Transplant Protocol]

Neuroanatomy Tutorial - http://library.med.utah.edu/WebPath/HISTHTML/NEURANAT/NEURANCA.html

Category: Neuroscience Protocols

This tutorial has images in which the structures are labelled. You are to identify the structures by clicking on the name of the structure. - [Read Neuroanatomy Tutorial]

Protocol Spindle Assembly In Vitro - http://www.bio.com/protocolstools/protocol.jhtml?id=p1051

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes an in vitro reaction to assay mitotic spindle assembly. The assay uses Cytostatic Factor extract made from Xenopus eggs, fluorescently-labelled tubulin, and prepared sperm nuclei. Spindle assembly is monitored by immunofluorescence microscopy. - [Read Protocol Spindle Assembly In Vitro]

Staining Resin Sections of Bone Protocol - http://www.nottingham.ac.uk/pathology/protocols/resbonestain.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for staining resin sections of bone. Includes: VON KOSSA/VAN GIESON; GOLDNER TRICHROME (MODIFICATION); TOLUIDINE BLUE; PERLS PRUSSIAN BLUE; SECTIONS LABELLED WITH TETRACYCLINE; FLATTENING FREE-FLOATING SECTIONS; MOUNTING FREE-FLOATING SECTIONS; CLEANING SLIDES. - [Read Staining Resin Sections of Bone Protocol]

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes - http://www.sanger.ac.uk/HGP/methods/cytogenetics/two_colour.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes. Sanger Institute - [Read Two-colour detection for combinations of biotinylated and digoxigenin-labelled probes]

Xenopus Whole Mount In Situ Hybridization Protocols - http://tropicalis.berkeley.edu/home/gene_expression/in-situ/insitu.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for Xenopus whole mount in situ hybridization. Includes: Protocols for Making Antisense RNA probe (dig labelled); Protocols for In Situ Hyb. - [Read Xenopus Whole Mount In Situ Hybridization Protocols]