koshland Protocols

Search results for: koshland

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Boiling Protein Prep Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/proteinprep.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Preparation of protein extracts for western blot. - [Read Boiling Protein Prep Protocol]

CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/chipmod.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

SPHEROPLAST CELLS, WASH SPHEROPLASTS, SONICATE SPHEROPLASTS, CHROMATIN SOLUTION, SET UP IMMUNOPRECIPITATIONS, REVERSE FORMALDEHYDE CROSSLINKS. Paul - Koshland lab. - [Read CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST]

DAPI Staining Yeast Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/dapistain.html

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for DAPI staining yeast. - [Read DAPI Staining Yeast Protocol]

Fluorescence in situ Hybridization Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/fish.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol for fluorescence in situ hybridization. - [Read Fluorescence in situ Hybridization Protocol]

Frozen Competent E.Coli Cells - http://www.ciwemb.edu/labs/koshland/Protocols/BACTERIA/ecolicells.html

Category: Microbiology: Bacterial Competent Cells Preparation E.Coli

Frozen Competent E.Coli Cells. Methods and protocol for freezing E.Coli Bacteria Competent Cells in aliquots for later use re-use. (Inoue et al., 1990 Gene 96:23). Koshland. - [Read Frozen Competent E.Coli Cells]

GFP Fixation in Yeast Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/gfpfix.html

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for GFP fixation in Yeast. - [Read GFP Fixation in Yeast Protocol]

Making Microtubule Seeds Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mtseeds.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for making microtubule seeds. - [Read Making Microtubule Seeds Protocol]

Microtubule Staining Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/microtubstain.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for microtubule staining. - [Read Microtubule Staining Protocol]

Minichromosome Microtubule BInding Assay Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/mmb.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Protocol for minichromosome microtubule binding assay. - [Read Minichromosome Microtubule BInding Assay Protocol]

No Boil Yeast Extracts Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/noboil.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for no boil Yeast extracts. - [Read No Boil Yeast Extracts Protocol]

Protocol for Cycling Tubulin - http://www.ciwemb.edu/labs/koshland/Protocols/MICROTUBULE/cyclingmicrotub.html

Category: Cell Biology and Cell Culture

Protocol for cycling tubulin using the buffers Glycerol PB and BRB80. - [Read Protocol for Cycling Tubulin]

Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/missegscreen.html

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for a rapid screen for chromosome missegregation mutants by DAPI staining. - [Read Rapid DAPI Screen for Chromosome Missegregation Mutants Protocol]

SDS-PAGE - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/sdspage.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE: gel electrophoresis of proteins. Very simple protocol for SDS-PAGE. Koshland Lab, Carnegie Institution of Washington, Howard Hughes Medical Institute. - [Read SDS-PAGE]

YAC Transfer by KAR1Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/YACTransfer.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for YAC transfer by KAR1. Includes: YAC transfer by Kar1 mating; Transfer YAC from donor cells into YPH 925; Transfer YAC from YPH925 into recipient cells. - [Read YAC Transfer by KAR1Protocol]