klenow Protocols

Search results for: klenow

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of E. coli DNA Polymerase - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3802

Category: DNA Protocols: DNA Sequencing Protocols: Dideoxy Mediated Sequencing Protocols

Protocol for dideoxy-mediated sequencing reactions using the Klenow fragment of E. coli DNA polymerase I and single-stranded DNA templates. - [Read Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of E. coli DNA Polymerase]

Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of E. coli DNA Polymerase I - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3802

Category: E Coli Protocols: E coli Nucleic Acid Protocols

Protocol for dideoxy-mediated sequencing reactions using the Klenow Fragment of E. coli DNA polymerase I and single-stranded DNA templates. - [Read Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of E. coli DNA Polymerase I]

EMSA using ds Oligonucleotides - http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

EMSA probe creation using ds Oligonucleotides by annealing two complementary oligos, and Klenow for probe creation. - [Read EMSA using ds Oligonucleotides]

Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3860

Category: Nucleic Acid Modification Protocols: Nucleic Acid Radiolabeling Protocols

In this procedure, synthesis of cDNA is carried out in the presence of saturating concentrations of all four dNTPs and trace amounts of a single radiolabeled dNTP. After subtraction hybridization, the enriched single-stranded cDNA is radiolabeled to high specific activity in a second synthetic reaction by extension of random oligonucleotide primers using the Klenow fragment of E. coli DNA polymerase. - [Read Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension Protocol]

Synthesis of Single-stranded DNA Probes of Defined Length from Bacteriophage M13 Templates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4061

Category: Viral Manipulation Protocols: M13 Phage Manipulation Protocols

A synthetic oligonucleotide annealed to single-stranded DNA derived from a recombinant bacteriophage M13 or phagemid template is used to prime the synthesis of complementary radiolabeled DNA. Synthesis is catalyzed by the Klenow fragment of E. coli DNA polymerase I, which extends the annealed primer for various distances along the single-stranded template DNA. - [Read Synthesis of Single-stranded DNA Probes of Defined Length from Bacteriophage M13 Templates Protocol]

Articles (1-1 of 1)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.