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Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol - http://www.animal.ufl.edu/hansen/Protocols/Immunohistochemistry_paraffin.htm

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol used to for immunohistochemistry on paraffin-embedded sections. Based on use of microwave energy to effect antigen retrieval. The immunohistochemistry procedure, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole (AEC) as chromogen. Undoubtedly, other kits or home-made reagents will also work . - [Read Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol]

Construction of cDNA Libraries in Eukaryotic Expression Vectors for Construction and Screening - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4068

Category: DNA Protocols: Genomic DNA Library Protocols

This stage summarizes how to construct a cDNA library in a mammalian vector, using commercial kits. These kits are available from several manufacturers and generally include all the required reagents. - [Read Construction of cDNA Libraries in Eukaryotic Expression Vectors for Construction and Screening]

Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples - http://www.ambion.com/techlib/tn/131/6.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Three Ambion kits were used to quantitate specific miRNAs and to detect differential miRNA expression in various mouse brain regions and cell types isolated by laser capture microdissection (LCM). These techniques can be applied to studying miRNA in other species, tissues, and cell types. Includes: Obtain Laser Capture Microdissected Samples; Isolate miRNA from LCM Samples; Quantitate miRNA by qRT-PCR. - [Read Detect and Quantitate MicroRNA in Laser Capture Microdissection Samples]

Fam Caspase 8 and 9 Binding Assay for Embryos Protocol - http://www.animal.ufl.edu/hansen/Protocols/caspase_8_and_9_binding_assay.htm

Category: Developmental Biology: Embryology Protocols

The FAM caspase binding assay kits from ATCC Corporation can be used to determine amounts of active caspases in cells. The FAM-labeled caspase inhibitor can freely diffuse into the cell. Active caspase irreversibly binds the inhibitor. Upon washing the cells, the amount of fluorescence is proportional to the amount of active caspase in the cell. FAM-LETD-fmk (catalog no. 30-1306) is used to detect caspase 8 and FAM-LEHD-fmk (catalog no. 30-1308) is used for caspase 9. - [Read Fam Caspase 8 and 9 Binding Assay for Embryos Protocol]

Kitchen Culture Kits - Plant Tissue Culture Resources - http://www.home.turbonet.com/kitchenculture/tcinfo.htm

Category: Plant Biology Protocols: Plant Tissue Culture

Cryopreservation protocols for different kinds of tissue of banana ... Teaching Plant Tissue Culture: University of Liverpool ... - [Read Kitchen Culture Kits - Plant Tissue Culture Resources]

Mitogen Activated Protein Kinase Guide - http://www.promega.com/guides/sigtrans_guide/strg_chap01.pdf

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Mitogen Activated Protein Kinase Guide. MEK, MEKK, MAPK, MAPKK, antibodies and kits Promega. - [Read Mitogen Activated Protein Kinase Guide]

Natural History Museum Protocol for EST sequencing from lambda zap phage plaques - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/nhm_est_method.html

Category: RNA Protocols: cDNA Libraries Library

(by PCR of phage insert, cycle sequencing using ABI dye terminator kits and automated sequencing on an ABI 373A) Ian Ridgers - [Read Natural History Museum Protocol for EST sequencing from lambda zap phage plaques]

Phage Display Libraries Kits - NEB - http://search.neb.com/query.html?qt=phage+display&Image1.x=0&Image1.y=0

Category: Peptide Protocols: Phage Display Cloning Systems and Protocols

Phage Display Libraries Kits - NEB (New England Biolabs) - [Read Phage Display Libraries Kits - NEB]

Pierce Protein Glycosylation Kits - http://www.piercenet.com/Products/Browse.cfm?fldID=7CE3FCF5-0DA0-4378-A513-2E35E5E3B49B

Category: Molecular Biology Protocols: Carbohydrate Protocols

Pierce Protein Glycosylation Kits and Protocols. Pierce - [Read Pierce Protein Glycosylation Kits]

Precipitation of SKY Kits and QC Protocol - http://www.riedlab.nci.nih.gov/publications/Precip%20SKY%20Kits%20&%20QC.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for precipitation of SKY Kits and QC. - [Read Precipitation of SKY Kits and QC Protocol]

Rapid Elution of DNA Agarose Gels Protocol - http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/dna_spin_ext.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

Rapid Elution of DNA Agarose Gels Protocol. This method allows quick purification of DNA fragments from agarose gels for use in cloning and other reactions. Higher yields of purified DNA can be obtained from commercially available purification kits, however greater ligation efficiencies per given amount of DNA have been seen with the use of these described spin columns. Hahn Lab. - [Read Rapid Elution of DNA Agarose Gels Protocol]