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Chromosome Spreads from Flower Buds of Arabidopsis thaliana Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1101

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Method is for preparing chromosomes from single flower buds of A. thaliana. It does not kill the plants allowing the determination of their chromosome number throughout development. Includes: Preparations of Arabidopsis; Preparation of Chromosome; Staining Chromosomes. - [Read Chromosome Spreads from Flower Buds of Arabidopsis thaliana Protocol]

Fungal DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]

Induction and Measurement of Cytotoxic T Lymphocyte Activity Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E663458DAAA03E5A23DC69D6BE8BE3E&objectid=6674B15EE255310CC4C2307B31AE3411

Category: Immunology: T Cell Protocols

Describes generating CTL against some commonly used target antigens. Two methods for the quantitation of CTL activity are described based on the two pathways used bt CTL to kill target cells. In one pathway, they release lytic granules containing perforin and granzymes, leading to apoptosis and target cell lysis. In a second pathway, they trigger apoptosis via Fas/Fas ligand interactions. - [Read Induction and Measurement of Cytotoxic T Lymphocyte Activity Protocol]

Kill Bed Bugs - http://www.killbedbugs.org

Category: Affiliates

Kill Bed Bugs. Information on how to treat, control and manage bedbug infestations. - [Read Kill Bed Bugs]

Articles (1-2 of 2)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

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