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AS Ammonium Sulfate Binding Assay for Cells - http://www.hopkinsmedicine.org/cellbio/devreotes/as.htm

AS Ammonium Sulfate Binding Assay for Cells. Devreotes Lab, John Hopkins Medical Centre. - [Read AS Ammonium Sulfate Binding Assay for Cells]

CHIFF preparation - http://www.hopkinsmedicine.org/cellbio/devreotes/chiff.htm

Category: Immunology: Chemotaxis

CHIFF preparation. Chemotaxis Assay protocol. Devreotes Lab, John Hopkins Medical Centre. - [Read CHIFF preparation]

Dynamic Flow Assay in a Parallel Plate Flow Chamber - http://www.glycotech.com/protocols/proto7.html

Category: Cell Biology and Cell Culture: Cell Adhesion Protocols

Flow assays allow visualization of cell adhesion under well-defined wall shear stress. Visualization of the events of cell adhesion are quantified by selective image acquisition and image processing. Events subsequent to the initial events can be studied such as cell stabilization and spreading. John T. Patton~GlycoTech Corporation, Rockville, Maryland - [Read Dynamic Flow Assay in a Parallel Plate Flow Chamber]

General Guide for Cryogenically Storing Animal Cell Cultures - http://www.corning.com/lifesciences/technical_information/techdocs/cryoanimalcc.asp

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Advantages of Freezing Cell Cultures, Practical Aspects of Cell Freezing, Cryoprotection, Storage Vessels, Labeling and Recordkeeping, Recovery, Managing a Cell Repository, cell selection, Problem Solving Suggestions. Corning. by John A. Ryan - [Read General Guide for Cryogenically Storing Animal Cell Cultures]

GTPÎ³S-induced Inhibition of Binding - http://www.hopkinsmedicine.org/cellbio/devreotes/gtp.htm

Category: Immunology: Chemotaxis

GTPÎ³S-induced Inhibition of Binding. Chemotaxis assay protocols. Devreotes Lab, John Hopkins Medical Centre. - [Read GTPÎ³S-induced Inhibition of Binding]

Immunostaining Thin Layer Chromatograms Of Glycolipids - http://www.glycotech.com/protocols/Proto5.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Immunostaining thin layer chromatograms TLC is a very sensitive detection technique of functionally active carbohydrate ligands of protein receptors. Carbohydrate structures are detected in glycolipids from complex mixtures of molecules extracted from the relevant target tissue. Proteins analyzed can be antibodies, chimeric Ig proteins, selectins, lectins, toxins, and other carbohydrate binding proteins. John L. Magnani~GlycoTech Corporation, Rockville, Maryland - [Read Immunostaining Thin Layer Chromatograms Of Glycolipids]

John Hopkins Animals Research Use and Care - http://www.jhu.edu/animalcare/training_procedures_animal_numbers.html

Category: Animal Research Techniques

Minimization of Animal Numbers Used to Obtain Valid Results. - [Read John Hopkins Animals Research Use and Care]

Measurement of Cell Adhesion Under Static Conditions - http://www.glycotech.com/protocols/Proto8.html

Category: Cell Biology and Cell Culture: Cell Adhesion Protocols

Many proteins and molecules promote cell adhesion including several cell surface carbohydrate binding proteins. Cell adhesion measurements on 96-well microtiter plate format are difficult due to the shear forces generated by washing the wells. The protocol here introduces the use of a liquid-filled wash chamber that separates unbound cells by gravity. This eliminates uncontrolled shear forces and passage of adherent cells through a liquid/air interface. John L. Magnani~GlycoTech Corporation. - [Read Measurement of Cell Adhesion Under Static Conditions]

Needle Assay for Chemotaxis - http://www.hopkinsmedicine.org/cellbio/devreotes/needle.htm

Category: Immunology: Chemotaxis

Needle Assay for Chemotaxis. Devreotes Lab, John Hopkins Medical Centre. - [Read Needle Assay for Chemotaxis]

Pouring Plates - http://rothlab.ucdavis.edu/protocols/M10.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Pouring Plates. Bacterial culture LB. John Roth, U.C Davis. - [Read Pouring Plates]

Top Agar Preparation - http://rothlab.ucdavis.edu/protocols/M11.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Prepare Top Agar and pouring plates for top agar. John Roth, U.C Davis. - [Read Top Agar Preparation]