isolation Protocols

Search results for: isolation

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]

Total RNA isolation (Guanidinium Thiocyanate-Phenol-Chloroform Extraction) Protocol - http://www.flemingtonlab.com/Protocols/TotalRNAprep.pdf

Category: Nucleic Acid Purification Protocols: RNA Isolation Protocols

Protocol for total RNA isolation. This method is fast and works well for preparation of total RNA. We typically use total RNA for quantitative S1 nuclease analysis of endogenous (or in vitro) gene expression, and this method works well for this purpose. - [Read Total RNA isolation (Guanidinium Thiocyanate-Phenol-Chloroform Extraction) Protocol]

Ultra-Fast Method of DNA Extraction from Neurospora Protocol - http://www.fgsc.net/fgn/camb.html

Category: Fungi Protocols: Neurospora Protocols

Simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation. - [Read Ultra-Fast Method of DNA Extraction from Neurospora Protocol]

Western Blot Analysis of Endogenous Gene Expression Protocol - http://www.flemingtonlab.com/Protocols/WestBlotAnalyofEndogProts.pdf

Category: Western Blot Protocols

Procedure describes how it denatures most of the modification and degradation proteins immediately giving the most accurate read out of the true levels of protein at the time of harvest. However, in cases where detection is a problem, a limited purification (e.g. isolation of nuclear extract for the detection of transcription factors) might be required to allow analysis. - [Read Western Blot Analysis of Endogenous Gene Expression Protocol]

Worthington Tissue Dissociation Guide - http://www.tissuedissociation.com/techniques.html

Category: Cell Culture Protocols

Includes information on: Cell Isolation Theory ( Tissue Types: Epithelial Tissue, Connective Tissue); Dissociating Enzymes (Collagenase, Trypsin, Elastase, Hyaluronidase, Papain, Chymotrypsin, Deoxyribonuclease I, Neutral Protease (Dispase), Trypsin Inhibitor (Soybean)); Cell Isolation Techniques: Working With Enzymes, Basic Primary Cell Isolation, Equilibration with 95%O2:5%CO2, Trituration, Enzymatic Cell Harvesting, Cell Adhesion and Harvesting, Trypsin for Cell Harvesting, etc... - [Read Worthington Tissue Dissociation Guide]

Xenopus RNA Isolation Protocol - http://spot.colorado.edu/~klym/Methods/RNAisolation.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus RNA isolation. - [Read Xenopus RNA Isolation Protocol]

Yeast DNA Isolation Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/yacs.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Yeast DNA Isolation Protocol for FISH. Sanger Institute. - [Read Yeast DNA Isolation Protocol for FISH]

Yeast DNA Isolation: Midiprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4147

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast DNA isolation. Protocol describes the preparation of DNA from a 40-ml liquid culture. - [Read Yeast DNA Isolation: Midiprep Protocol]

Yeast DNA Isolation: Miniprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4148

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of DNA from a 5-ml liquid culture of yeast. - [Read Yeast DNA Isolation: Miniprep Protocol]

Yeast Genomic DNA Isolation Procedure - http://www.upstate.edu/biochem/amberg/protocols/yeast_genomic_DNA.html

Category: DNA Protocols: DNA Extraction Protocols

Yeast Genomic DNA Isolation David Amberg, Upstate Medical University. - [Read Yeast Genomic DNA Isolation Procedure]

Yeast RNA Isolation: Large-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4154

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes large-scale yeast RNA isolation. It is designed to yield more than 10 mg of total nucleic acid and 300-400 µg of poly(A)-selected mRNA from each 500-ml culture. - [Read Yeast RNA Isolation: Large-Scale Protocol]

Yeast RNA Isolation: Small-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4155

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes rapid, small-scale yeast RNA isolation. It is based on the work of Schmitt et al. (1990). Note that all containers should be washed in Rnase Away (Invitrogen) or dry baked for 24 hours at 160°C. - [Read Yeast RNA Isolation: Small-Scale Protocol]

Articles (1-5 of 5)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

DNA Fragment Purification from Polyacrylamide Gels Protocol

A simple protocol for the purification of DNA fragments from Polyacrylamide Gels.

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

[1-5]

Items 176-187 out of 187 displayed.

First Previous 1 2 3 4 5 6 7 [8] Last

Total records: 187