irritation Protocols

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Agarose Overlay Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/31.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Protocol descibes the use of L929 mouse fibroblast cells cultured in vitro in an agarose overlay assay to assess the toxicity of test substances. The assay may be useful in assessing the irritation potential of test substances (e.g. surfactant-based products) as an alternative to the Draize rabbit eye test. - [Read Agarose Overlay Assay Protocol]

Mammalian Toxicology Acute Toxicity Studies - http://www.sri.com/biosciences/bdd/toxic/protocol.html#1

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Mammalian toxicology for acute toxicity studies. Includes: M101 Acute Limit Test (Rodents), M102 Acute Toxicity Study (Rats or Mice), M120 Dose Escalation Study (Dog), M103 Acute Dermal Limit Test, M104 Acute Dermal (LD50), Study M105 Primary Dermal Irritation, M106 Primary Eye Irritation Study, M106-A, M110 Local Lymph Node Assay in Mouse, M115 Rabbit Vaginal Irritation Assay. - [Read Mammalian Toxicology Acute Toxicity Studies]

Red Blood Cell Test System Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/37.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

An in vitro red blood cell assay is presented which allows the estimation of the irritation potential of tensides and tenside containing materials such as shampoos, shower gels, cleaning products, etc. The estimation is based on the fact that surfactants interact strongly with cellular membranes and proteins. Both effects are measured photometrically by use of the inherent native dye, oxyhemoglobin. - [Read Red Blood Cell Test System Protocol]

SIRC Cytotoxcitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/40.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Rabbit-derived corneal cells are cultured in the presence of test compounds, the toxicity of which are determined by their effect upon cell viability. A decrease in cell number, as measured by uptake of the dye Neutral Red, serves as an indicator of potential cytotoxicity. This test has been proposed as a potential replacement alternative for the Draize Eye Irritation test. - [Read SIRC Cytotoxcitiy Test]